Ezh1 (BC007135) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0388
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210461 representing BC007135 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAAT CTGAGTATATGCGGCTTCGACAGCTCAAACGGCTCCAGGCAAATATGGGAGCAAAGGCTCTGTATGTGGC AAATTTTGCAAAGGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGTGTCCAGCCT GTTCAGCCAATGAAGCCCGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCAG GGTTCGACAGCCAGGATATGTTGATGCGGTCTCTGAACACTGTTGCACTGGTTCCCATCATGTATTCCTG GTCCCCTCTCCAGCAGAATTTCATGGTGGAAGATGAGACGGTTTTGTGCAATATTCCATACATGGGTGAC GAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGTG AAGAAGAGATGATCCCTGGATCTGTGCTGATCAGCGATGCTGTGTTTCTGGAGCTGGTGGATGCCCTCAA CCAGTACTCTGATGAGGAGGAGGACGGGCACAACGACCCCTCCGATGGAAAGCAAGACGACAGCAAAGAG GACCTGCCGGTAACAAGAAAACGGAAGCGCCATGCTATCGAAGGCAACAAAAAGAGTTCCAAGAAACAGT TTCCAAATGACATGATCTTCAGCGCCATTGCGTCCATGTTTCCTGAGAATGGTGTCCCTGACGACATGAA GGAGAGGTATCGAGAGCTGACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATC GATGGCCCCAACGCCAAGTCAGTGCAGCGGGAGCAGTCTCTGCACTCTTTCCACACCCTTTTCTGCCGGC GCTGTTTTAAATATGACTGCTTCCTTCACCCTTTCCACGCCACCCCAAATGTATATAAGCGCAAGAACAA GGAAATCAAGATTGAGCCAGAACCGTGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTAC GCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTT CCTGCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCAA TGACTGGGCCTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACCCCCACGAAACAGAAAGCCAGTCCA GCCCCAGCTCAGCTCTGTGTTGTGGAAGCCCCCTCAGAGCCGGTGGAATGGACCGGAGCCGAAGAATCTC TTTTCCGAGTCTTCCACGGCACCTATTTCAACAACTTCTGCTCAATAGCCAGGCTTCTGGGGACAAAGAC ATGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTAAAGCTGCCAACAGATGAGCTCATG AACCCTGCACAGAAGAAGAAAAGAAAACACAGGTTGTGGGCCGCACACTGCAGGAAAATTCAGCTGAAGA AAGATAACAATTCTACACAAGTGTATAACTACCAACCCTGTGACCACCCAGACCGTCCGTGTGACAGCAC ATGCCCCTGCATCATGACCCAGAACTTTTGTGAAAAGTTCTGCCAGTGCAGCCCAGACTGCCAGAATCGC TTTCCTGGTTGTCGCTGTAAGACTCAGTGCAATACCAAGCAATGTCCATGCTACTTGGCAGTTCGTGAGT GTGACCCTGACTTGTGCCTCACCTGTGGGGCCTCAGAGCACTGGGACTGTAAGGTGGTGTCTTGCAAAAA CTGCAGCATCCAGCGTGGCCTCAAAAAGCACCTGCTGCTGGCCCCTTCCGATGTGGCCGGATGGGGCACC TTCATCAAGGAGTCTGTGCAGAAGAATGAATTCATTTCTGAATATTGTGGTGAGCTCATCTCTCAGGATG AGGCTGATCGTCGAGGGAAGGTCTATGATAAATACATGTCTAGCTTCCTCTTCAACCTCAACAATGATTT TGTAGTGGATGCTACCCGGAAAGGAAACAAAATTCGCTTTGCAAACCATTCAGTGAACCCCAACTGTTAT GCCAAAGTGGTTATGGTGAATGGAGATCACCGCATTGGGATCTTTGCCAAGAGAGCAATTCAGGCTGGCG AAGAGCTCTTCTTTGATTATAGGTACAGCCAAGCTGATGCCCTCAAGTATGTGGGCATCGAGAGGGAAAC GGACGTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210461 representing BC007135 Red=Cloning site Green=Tags(s) MRKMDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQP VQPMKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGD EVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEDGHNDPSDGKQDDSKE DLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNI DGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEY AMLHNPRSKCSGRRRRRHPVVSASCSNASASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASP APAQLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELM NPAQKKKRKHRLWAAHCRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNR FPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGT FIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCY AKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC007135
ORF Size	2252 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC007135, AAH07135
RefSeq Size	4179 bp
RefSeq ORF	2252 bp
Locus ID	14055
Gene Summary	This gene encodes a member of the Polycomb-group (PcG) family. The encoded protein is interchangeable with the related Enhancer of zeste 2 (Ezh2) protein as a core component of the polycomb repressive complex 2 (PRC2), which methylates histone H3 at lysine 27 and results in the transcriptional repression of affected target genes. This complex is involved in carrying out cell-fate decisions during embryonic stem cell differentiation. [provided by RefSeq, Sep 2014]

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