Pigr (BC013556) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210561 representing BC013556 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCTACTTGTTCACGCTCTTGGTAACTGTCTTTTCAGGGGTCTCCACAAAAAGCCCCATATTTG GTCCCCAGGAGGTGAGTAGTATAGAAGGCGACTCTGTTTCCATCACGTGCTACTACCCAGACACCTCTGT CAACCGGCACACCCGGAAATACTGGTGCCGACAAGGAGCCAGCGGCATGTGCACAACGCTCATCTCTTCA AATGGCTACCTCTCCAAGGAGTATTCAGGCAGAGCCAACCTCATCAACTTCCCAGAGAACAACACATTTG TGATTAACATTGAGCAGCTCACCCAGGACGACACTGGGAGCTACAAGTGTGGCCTGGGTACCAGTAACCG AGGCCTGTCCTTCGATGTCAGCCTGGAGGTCAGCCAGGTTCCTGAGTTGCCGAGTGACACCCACGTCTAC ACAAAGGACATAGGCAGAAATGTGACCATTGAATGCCCTTTCAAAAGGGAGAATGCTCCCAGCAAGAAAT CCCTGTGTAAGAAGACAAACCAGTCCTGCGAACTTGTCATTGACTCTACTGAGAAGGTGAACCCCAGCTA TATAGGCAGAGCAAAACTTTTTATGAAAGGGACCGACCTAACTGTATTCTATGTCAACATTAGTCACCTA ACGCACAATGATGCTGGGCTGTACATCTGCCAAGCTGGAGAAGGTCCTAGTGCTGATAAGAAGAATGTTG ACCTCCAGGTGCTAGCGCCTGAGCCAGAGCTGCTTTATAAAGACCTGAGGTCCTCAGTGACTTTTGAATG TGACCTGGGCCGTGAGGTGGCAAACGAGGCCAAATATCTGTGCCGGATGAATAAGGAAACCTGTGATGTG ATCATTAACACCCTGGGGAAGAGGGATCCAGACTTTGAGGGCAGGATCCTGATAACCCCCAAGGATGACA ATGGCCGCTTCAGTGTGTTGATCACAGGCCTGAGGAAGGAGGATGCAGGGCACTACCAGTGTGGAGCCCA CAGTTCTGGTTTGCCTCAAGAAGGCTGGCCCATCCAGACTTGGCAACTCTTTGTCAATGAAGAGTCTACC ATTCCCAATCGTCGCTCTGTTGTGAAGGGAGTCACAGGAGGCTCTGTGGCCATCGCCTGTCCCTATAACC CCAAGGAAAGCAGCAGCCTCAAGTACTGGTGTCGCTGGGAAGGGGACGGAAATGGACATTGCCCCGTGCT TGTGGGGACCCAGGCCCAGGTGCAAGAAGAGTATGAAGGCCGACTGGCACTGTTTGATCAGCCAGGCAAT GGTACTTACACTGTCATCCTCAACCAGCTCACCACCGAGGATGCTGGCTTCTATTGGTGTCTTACCAATG GTGACTCTCGCTGGAGAACCACAATAGAACTCCAGGTTGCCGAAGCTACAAGGGAGCCAAACCTTGAGGT GACGCCACAGAACGCAACAGCAGTACTAGGAGAGACCTTCACCGTTTCCTGCCACTATCCGTGCAAATTC TACTCCCAGGAGAAATACTGGTGCAAGTGGAGCAACAAGGGTTGCCACATCCTGCCAAGCCATGACGAAG GTGCCCGCCAATCTTCTGTGAGCTGCGACCAGAGCAGCCAGCTGGTCTCCATGACCCTGAACCCGGTCAG TAAGGAAGATGAAGGCTGGTACTGGTGTGGGGTAAAGCAAGGCCAGACCTATGGAGAAACTACCGCCATC TATATAGCAGTTGAAGAGAGGACCAGAGGGTCATCCCATGTCAACCCAACAGATGCAAATGCACGTGCCA AAGTCGCTCTGGAAGAAGAGGTAGTGGACTCCTCCATCAGTGAAAAAGAGAACAAAGCCATTCCAAATCC CGGGCCTTTTGCCAACGAAAGAGAGATACAGAATGTGGGAGACCAAGCTCAGGAGAACAGAGCATCTGGG GATGCTGGCAGTGCTGATGGACAAAGCAGGAGCTCCAGCTCCAAAGTGCTGTTCTCCACCCTGGTGCCCC TGGGTCTGGTGCTGGCAGTGGGTGCTATAGCTGTGTGGGTGGCCAGAGTCCGACATCGGAAGAATGTAGA CCGCATGTCAATCAGCAGCTACAGGACAGACATTAGCATGGCAGACTTCAAGAACTCCAGAGATTTGGGA GGCAATGACAACATGGGGGCCTCTCCAGACACACAGCAAACAGTCATCGAAGGAAAAGATGAAATCGTGA CTACCACGGAGTGCACCGCTGAGCCAGAAGAATCCAAGAAAGCAAAAAGGTCATCCAAGGAGGAAGCTGA CATGGCCTACTCGGCATTCCTGCTTCAGTCCAGCACCATAGCTGCACAGGTCCACGATGGTCCCCAGGAA GCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210561 representing BC013556 Red=Cloning site Green=Tags(s) MRLYLFTLLVTVFSGVSTKSPIFGPQEVSSIEGDSVSITCYYPDTSVNRHTRKYWCRQGASGMCTTLISS NGYLSKEYSGRANLINFPENNTFVINIEQLTQDDTGSYKCGLGTSNRGLSFDVSLEVSQVPELPSDTHVY TKDIGRNVTIECPFKRENAPSKKSLCKKTNQSCELVIDSTEKVNPSYIGRAKLFMKGTDLTVFYVNISHL THNDAGLYICQAGEGPSADKKNVDLQVLAPEPELLYKDLRSSVTFECDLGREVANEAKYLCRMNKETCDV IINTLGKRDPDFEGRILITPKDDNGRFSVLITGLRKEDAGHYQCGAHSSGLPQEGWPIQTWQLFVNEEST IPNRRSVVKGVTGGSVAIACPYNPKESSSLKYWCRWEGDGNGHCPVLVGTQAQVQEEYEGRLALFDQPGN GTYTVILNQLTTEDAGFYWCLTNGDSRWRTTIELQVAEATREPNLEVTPQNATAVLGETFTVSCHYPCKF YSQEKYWCKWSNKGCHILPSHDEGARQSSVSCDQSSQLVSMTLNPVSKEDEGWYWCGVKQGQTYGETTAI YIAVEERTRGSSHVNPTDANARAKVALEEEVVDSSISEKENKAIPNPGPFANEREIQNVGDQAQENRASG DAGSADGQSRSSSSKVLFSTLVPLGLVLAVGAIAVWVARVRHRKNVDRMSISSYRTDISMADFKNSRDLG GNDNMGASPDTQQTVIEGKDEIVTTTECTAEPEESKKAKRSSKEEADMAYSAFLLQSSTIAAQVHDGPQE A TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC013556
ORF Size	2315 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013556, AAH13556
RefSeq Size	3560 bp
RefSeq ORF	2315 bp
Locus ID	18703
Gene Summary	This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment (By similarity).[UniProtKB/Swiss-Prot Function]

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