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Dgcr8 (NM_033324) Mouse Tagged ORF Clone
CAT＃: MG210572
- TrueORF®
Dgcr8 (tGFP-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8)
ORF Plasmid: DDK
"NM_033324" in other vectors (3)
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CNY 7,410.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D16H22S788E; D16H22S1742E; D16Wis2; Gy1; mir-1306; N41; Vo59c07 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210572 representing NM_033324
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACATATGAGAGTCCCTCTCCTCTCCCGCGTGAGCCCGCAGGAGAAGCGATGATGGAGAACCGAG CTTGCCCCTTCCAAGTGCTGCCCCATGAACAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGATGCAGA GGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACCTCCTGCCGACGACCCATTCAACTTCTAC GGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCC ACAGCCCGCGCACTGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGA TGTAAAGATTAGCGTGAGCTTTACTGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGTA GAACGCAGCACTCGGCCTGAGTGTGGCCAGCTCCTTAGTCCTGTCAGTGGGGACGTGCATGCTTGTCCCT TTGGCGGGAGTGTTGGTAATGGGGTAGGCCTAGGGGGTGAGAGTGCAGATAAGAAGGATGAGGAAAATGA GCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAG CTAGATGAAGAAGGAACAGGCGGGTTCACGGCTAAAGCAATCGTTCAAAGAGACAGAGTGGATGAAGAGG CCTTGAATTTCTCCTATGAGGATGACTTTGACAATGATGTGGACGCTTTACTAGAAGAAGGTCTCTGTGC TCCCAAGAAGAGGCGAATGGAGGAAAAATATGGCGGAGACAGTGATCATCCATCTGATGGAGAGACAAGT GTACAGCCAATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGTCCACCTACAGAGCCATTGC CTGATGGATGGATCATGACTTTTCATAATTCTGGAGTCCCTGTATACCTGCACAGAGAGTCTCGAGTGGT CACTTGGTCCAGACCCTACTTCTTGGGAACAGGAAGCATACGGAAACATGATCCTCCTCTAAGCAGTATC CCCTGCCTACATTATAAGAAAATGAAGGACAATGAGGAACGAGAACAAAACTGTGATCTTGCCCCCAGTG GAGAGGTGTCACCTGTCAAGCCCTTGGGTCGGTCTGCAGAGTTGGATTTCCCTCTGGAAGAGCCTGACTC CATGGGTGGAGACTCAGGGTCCATGGATGAGAAGGACCCATTGGGGGCTGAGGCAGCCGCTGGAGCCCTG GGACAAGTGAAGGCTAAAGTTGAGGTGTGCAAAGATGAATCAGTTGATCTGGAGGAATTTCGTAATTACC TTGAGAAGCGTTTTGACTTTGAACAAGTAACTGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGTCAGTT CAACCGTGAGATGAAGCGGAAGCAGGCCGAGTCAGAGAGGCCCATCCTGCCAGCCAACCAGAAGCTGATC ACTCTATCTGTACAAGATGCACCCACAAAGAAAGAGTTTGTCATCAATCCCAATGGGAAGTCTGAGGTTT GCATCCTGCACGAATACATGCAGCGTGTCCTCAAGGTCCGCCCTGTTTATAATTTCTTTGAATGTGAGAA TCCAAGTGAGCCTTTTGGTGCCTCCGTGACCATTGATGGTGTGACTTACGGATCTGGAACTGCAAGCAGC AAAAAACTTGCGAAGAATAAAGCTGCCCGAGCCACCCTGGAAATTCTCATCCCTGACTTTGTTAAACAGA CCTCTGAGGAGAAGCCTAAAGACAGTGAAGAACTGGAGTATTTTAACCACATCAGTATTGAGGATTCACG AGTCTATGAGCTGACAAGCAAGGCTGGGCTGTTGTCTCCATATCAGATCCTCCATGAGTGCCTTAAAAGA AACCATGGAATGGGTGACACATCCATCAAGTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATATG TTATGGCATGCGGCAAACACACAGTGCGCGGGTGGTGTAAGAATAAACGAGTTGGGAAACAATTAGCATC TCAGAAAATCCTTCAGCTACTGCACCCACATGTCAAGAACTGGGGTTCCTTACTACGCATGTATGGTCGT GAGAGCAGCAAAATGGTCAAGCAGGAGACCTCTGACAAGAGTGTGATAGAGCTACAGCAGTATGCCAAGA AGAACAGGCCCAACCTTCACATCCTGAGCAAGCTACAAGAAGAGATGAAGAGGCTGGCTGCAGAGCGGGA GGAGACTCGGAAGAAACCCAAGATGTCAATTGTAGCATCTGCCCAGCCTGGTGGTGAGCCCTTGTGCACA GTCGATGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210572 representing NM_033324
Red=Cloning site Green=Tags(s) METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFY GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGV ERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLE LDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETS VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI PCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGAL GQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR ESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCT VDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033324 |
| ORF Size | 2319 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033324.2, NP_201581.2 |
| RefSeq Size | 4226 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 94223 |
| UniProt ID | Q9EQM6 |
| Gene Summary | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210572 | Dgcr8 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8) |
CNY 5,672.00 |
|
| MR210572L3 | Lenti ORF clone of Dgcr8 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8) |
CNY 8,650.00 |
|
| MR210572L4 | Lenti ORF clone of Dgcr8 (mGFP-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8) |
CNY 8,650.00 |
