Dpp10 (BC067026) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	DPRP3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210711 representing BC067026 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGGAGCAGCAACCCACCCCAGGGGCCAGGGCAACCCAGTCGCAGCCAGCCGACCAGGAACTAG GAAGTAACAGCCCTCCACAAAGAAACTGGAAGGGAATCGCCATTGCCCTGCTGGTGATCTTGGTGGTATG CTCACTCATCACAATGTCTGTCATCCTGTTAACACCAGATGAACTAACAAATTCTTCAGAAACAAGACTG TCACTAGAGGAGCTTTTGGGGAAAGGATTCGGACTTCATAATCCAGAACCTCGGTGGATCAATGATACAG TCGTGGTATATAAAACCAACAATGGACACGTCATGAAACTGAATACAGAATCAAATGCTTCCACATTGTT ATTGGACAACTCAACTTTTGTAACGTTTAAAGCCTCCAGACACTCACTTTCCCCAGATTTAAAGTATGTT CTCCTGGCATACGACGTTAAGCAGATTTTTCATTACTCATTTACCGCTTCATATTTGATTTACAACATAC ACACTGGGGAAGTGTGGGAGTTGAATCCCCCAGAAGTTGAGGACTCAGTGTTGCAGTATGCGGCCTGGGG TGTGCAAGGACAGCAGCTGATTTATATTTTTGAAAACAATATCTACTATCAACCTGATATCAAGAGCAGT TCTTTACGACTAACATCTTCAGGAAAGGAAGGCATTATTTTTAATGGGATTGCTGACTGGTTATATGAAG AAGAACTTCTGCATTCTCATATCGCCCACTGGTGGTCCCCCGATGGAGAGAGACTTGCCTTCCTGATGAT AAATGACTCGTTGGTGCCTAACATGATCATACCTCGGTTTACTGGAGCACTGTACCCCAAAGCAAAGCAG TACCCATACCCCAAGGCAGGTCAAGCGAACCCATCAGTGAAGTTATATGTTGTAAACCTGTATGGACCAA CTCATACTTTGGAGCTGATGCCACCCGACATTTTTAAATCAAGAGAATATTACATCACCATGGTTAAATG GGTGAGCAACACGAGGACAGTGGTCAGGTGGTTGAATCGGCCTCAGAACATCTCCATCCTCACACTCTGT GAATCTACCACCGGGGCATGCAGCAGGAAATATGAGATGACGTCCGACACCTGGCTCTCTAAACAGAACG AGGAGCCAGTGTTTTCCAGAGATGGAAGCAAGTTCTTCATGACTGTTCCTGTTAAACAAGGTGGAAGAGG AGAATTCCACCACATAGCTATGTTCCTGGTCCAGAGTAAAAGCGAGCAAATTACGGTGCGGCATCTGACA TCAGGAAACTGGGAAGTGATAAGGATCTTGGCCTATGATGAAACAACTCAAAAAATTTACTTTCTGAGCA CAGAATCTTCTCCCCAAGGGAGGCAACTATACAGTGCTTCTACTGAGGGACTATTGAATCGTGATTGCAT CTCATGCAACTTTATGAAAGAAGATTGCACGTATTTTGATGCCAGCTTTAGCCCCATGAATCAACATTTC TTGTTATTCTGTGAAGGTCCAAAGGTCCCAGTGGTCAGCCTTCACATCACAGACAACCCATCAAGGTATT TCCTCTTGGAAAACAATTCTGTGATGAAAGAAACTATTCAGAAGAAGAAGCTCGCAAAGAGAGAGACTAG AATACTTCACATTGATGACTATGAACTTCCTTTACAGTTGTCCTTTCCCAAAGATTTTATGGAGAAAAAC CAGTATGCTCTTCTATTAATAATGGATGAAGAACCAGGAGGCCAAATGGTGACAGATAAGTTCCATGTTG ACTGGGATTCAGTTCTTATTGACACCGATAATGTCATTGTAGCAAGATTTGATGGCAGAGGAAGTGGATT CCAGGGCCTGAAAGTTTTGCAGGAGATTCACAGAAGGATAGGCTCAGTGGAGGCAAAGGACCAAGTAGCT GCTGTAAAATATTTACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTTGGAAAGGGATATG GGGGCTATATTGCATCAATGATCTTAAAATCAGATGAGAAGTTTTTCAAATGTGGAGCCGTGGTTGCACC CATCTCAGACATGAAGTTGTATGCCTCAGCTTTCTCTGAACGGTACCTTGGCATGCCATCAAAGGAAGAA AGCACTTACCAGGCATCCAGTGTACTGCATAACATTCATGGTTTAAAAGAAGAAAATTTATTAATAATTC ACGGAACTGCTGATACAAAAGTTCATTTCCAGCATTCAGCAGAATTGATCAAGCATCTGATAAAAGCTGG GGTGAATTACACTCTGCAGGTCTATCCAGATGAAGGATATCACATTTCAGACAAGAGCAAGCATCATTTT TACAGCACAATTCTCAGATTCTTCAGTGATTGTCTAAAGGAAGAGGTATCTGTGCTGCCACAGGAACCAG AAGAAGATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210711 representing BC067026 Red=Cloning site Green=Tags(s) MKQEQQPTPGARATQSQPADQELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSETRL SLEELLGKGFGLHNPEPRWINDTVVVYKTNNGHVMKLNTESNASTLLLDNSTFVTFKASRHSLSPDLKYV LLAYDVKQIFHYSFTASYLIYNIHTGEVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSS SLRLTSSGKEGIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPNMIIPRFTGALYPKAKQ YPYPKAGQANPSVKLYVVNLYGPTHTLELMPPDIFKSREYYITMVKWVSNTRTVVRWLNRPQNISILTLC ESTTGACSRKYEMTSDTWLSKQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLVQSKSEQITVRHLT SGNWEVIRILAYDETTQKIYFLSTESSPQGRQLYSASTEGLLNRDCISCNFMKEDCTYFDASFSPMNQHF LLFCEGPKVPVVSLHITDNPSRYFLLENNSVMKETIQKKKLAKRETRILHIDDYELPLQLSFPKDFMEKN QYALLLIMDEEPGGQMVTDKFHVDWDSVLIDTDNVIVARFDGRGSGFQGLKVLQEIHRRIGSVEAKDQVA AVKYLLKQPYIDSKRLSIFGKGYGGYIASMILKSDEKFFKCGAVVAPISDMKLYASAFSERYLGMPSKEE STYQASSVLHNIHGLKEENLLIIHGTADTKVHFQHSAELIKHLIKAGVNYTLQVYPDEGYHISDKSKHHF YSTILRFFSDCLKEEVSVLPQEPEEDE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC067026
ORF Size	2393 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC067026, AAH67026
RefSeq Size	4634 bp
RefSeq ORF	2393 bp
Locus ID	269109
Gene Summary	Promotes cell surface expression of the potassium channel KCND2 (PubMed:22311982). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:22311982). Has no dipeptidyl aminopeptidase activity (Probable).[UniProtKB/Swiss-Prot Function]

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