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Anapc4 (NM_024213) Mouse Tagged ORF Clone
CAT＃: MG210765
- TrueORF®
Anapc4 (tGFP-tagged) - Mouse anaphase promoting complex subunit 4 (Anapc4)
ORF Plasmid: DDK
"NM_024213" in other vectors (3)
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CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610306D21Rik; APC4; D5Ertd249e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210765 representing NM_024213
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCTTTCCGACCTGTTTCCCATCCTTTCGGGTGGTGGGAGAGAAGCAGCTGCCACAGGAGATTA TTTTCTTGGCCTGGTCCCCCAAGCGAGACCTCATTGCTTTGGCCAACACTACAGGCGAGGTTTTGCTGCA TCGCCTTGCAAGTTTTCATCGAGTTTGGAGTTTCCCACCAAATGAAAGTACAGGAAAAGAAGTGACCTGC TTGGCCTGGAGACCAGACGGCAAACTTTTGGCCTTTGCTCTTGCGGATACCAAGAAAATTATTTTGTGTG ATGTAGAAAAGCCTGAAAGCTTACACTCCTTCTCTGTGGAGGCTCCGGTCTCTTGTATGCATTGGACAGA AGTGACTGTGGAAAGCAGTGTTTTAACATCGTTTTATAATGCTGAGGATGAGTCCAATCTTCTCTTGCCT AAGCTGCCCACATTGCCAAAAAATTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAA TTATCAAGCTCTTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCT TTATGCTTATGGGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTA TCAAGTGATTTGAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATT TTCAGCTTGAAACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCA CATTTCAGCTCTCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTG ATGCAGATGGACTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGT TCATGCACTTGCTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGT GAAGGGCTTAAAAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGT CACTTACAGAGTGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAAC AAAAATACGAACCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCAT CCTCAAAGCAAACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGG CTGTATGTAGCAATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGG ATATCACATTTGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGG AAAATACTTCAATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCT AACACAGAAGGAAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGT TTCCTTATTACCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTT GCAAAAGCCAGCAGATGTAATTGGAAGATCGATGAATCAAGCAATTTGCATTCCATTATATAAAGATGCT AGGAGTATGGACTGTGCACGAAGATTGTTAAAATTCCCATTTCTGTGGAATAATAAAACTTCGAATCTTC ATTATCTTCTTTTTACTATTTTAGAAGATTCGGTTTATAAAATGTGCATCTTAAGGAGACATACTGATAT TTCCCAATCTGTAAGTAATGGACTAATTGGTATTAAATTTGGGAGCTTCACATCTGCCTCAGCTGACAAG GTCAGAAGAAGCTCTTACAGTTGTCTAGACGCCCAGTTCTATGATGATGAGACTGTCACGGTCATCCTGA AGGACTCCATGGGACGTGAAGGGAGAGACAGGATTTTGGTTCAGTTGTCACTGTCCTTGGTGTATAACAG TGAAGATTCTGACGAATATGAGTTCACTGGGAGTTACTCGACAAGGCTTGACGAGCAGGGCAGCATTATC CCCACACGCACCATGCACTTTGAGAAACACTGGAGGCTGCTAGAAAGCATGAGAGCGCAGTATGTTGCTG GGAACGGCCTTCGAAAAGTGTCCTGTGTGTTAAGTTCAAACCTCCGGCATGTGAGGGTGTTTGAAATGGA CATCGATGATGAGTGGGAGATTGACGAGTCTTCAGACGATGAGGAGGAGGCTGGCGGGAAGCCTGTGAAG ATCAAGGAGGAAGTGTTGTCAGAGTCAGAGACAGAGGCTCACCAAGACGCTGCTGCCCTAGACCCGGACG TAGTCATCAAAGTGGAGAAACTTGACCCTGAGTTGGACTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210765 representing NM_024213
Red=Cloning site Green=Tags(s) MLRFPTCFPSFRVVGEKQLPQEIIFLAWSPKRDLIALANTTGEVLLHRLASFHRVWSFPPNESTGKEVTC LAWRPDGKLLAFALADTKKIILCDVEKPESLHSFSVEAPVSCMHWTEVTVESSVLTSFYNAEDESNLLLP KLPTLPKNYNSTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCIALCL SSDLKSLSVVTEVSSGGESEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEIL MQMDSRLTKFVQEKPTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVIS HLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEDAITAVGSFILKANELLQVIDSSMKNFKAFFRW LYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPP NTEGNQWYDFLQNSTHLKESPLLFPYYPRKSLHFVKRRMENVIDQCLQKPADVIGRSMNQAICIPLYKDA RSMDCARRLLKFPFLWNNKTSNLHYLLFTILEDSVYKMCILRRHTDISQSVSNGLIGIKFGSFTSASADK VRRSSYSCLDAQFYDDETVTVILKDSMGREGRDRILVQLSLSLVYNSEDSDEYEFTGSYSTRLDEQGSII PTRTMHFEKHWRLLESMRAQYVAGNGLRKVSCVLSSNLRHVRVFEMDIDDEWEIDESSDDEEEAGGKPVK IKEEVLSESETEAHQDAAALDPDVVIKVEKLDPELDS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024213 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024213.2, NP_077175.1 |
| RefSeq Size | 2668 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 52206 |
| UniProt ID | Q91W96 |
| Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210765 | Anapc4 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 4 (Anapc4) |
CNY 8,080.00 |
|
| MR210765L3 | Lenti ORF clone of Anapc4 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 4 (Anapc4) |
CNY 9,980.00 |
|
| MR210765L4 | Lenti ORF clone of Anapc4 (mGFP-tagged) - Mouse anaphase promoting complex subunit 4 (Anapc4) |
CNY 9,980.00 |
