United States
Germany
Japan
United Kingdom
China
Stt3b (NM_024222) Mouse Tagged ORF Clone
CAT＃: MG210829
- TrueORF®
Stt3b (tGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b)
ORF Plasmid: DDK
"NM_024222" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300006C19Rik; Simp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210829 representing NM_024222
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGCGGCCTCA TGGCTCTGGGGAACAGCCGCCACGGGCACCATGGGCCCGGAACCCAGAGCGCGTCCAGGGCGGCGGCGCC GAAGCCGGGGCCCCCCGCGGGGCTGTCCGGGGGCTTGTCGCAGCCGGCCGGGTGGCAGTCGTTGCTCTCC TTCACCATCCTCTTCCTGGCCTGGCTGGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAGA GCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGATTCTA TGAGTTTCTAAATTGGTTTGATGAAAGAGCATGGTACCCACTGGGAAGAATAGTGGGTGGCACCGTTTAC CCAGGGTTGATGATAACAGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACAGTTCACATAA GAGATGTGTGTGTATTCCTTGCACCAACTTTTAGCGGCCTTACATCCATATCTACGTTCCTGCTAACTAG AGAACTGTGGAACCAAGGAGCAGGACTTCTAGCTGCTTGCTTCATTGCTATCGTACCAGGGTACATATCT CGGTCAGTGGCGGGATCCTTTGATAATGAAGGCATTGCCATTTTTGCGCTTCAGTTCACTTACTACTTAT GGGTAAAGTCTGTGAAGACCGGGTCTGTGTTCTGGACAATGTGCTGCTGCTTGTCATATTTCTACATGGT CTCTGCGTGGGGAGGTTATGTGTTCATCATCAACCTCATCCCTCTCCATGTGTTTGTGTTGCTGCTGATG CAGAGGTACAGCAAGAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATATTATCCATGC AGATACCTTTTGTGGGATTTCAGCCAATCAGAACAAGCGAGCACATGGCAGCTGCAGGTGTCTTTGCGCT GCTGCAAGCTTACGCTTTTTTGCAGTATCTGAGAGACCGGTTGACAAAACAGGAGTTCCAGACCCTTTTC TTTTTGGGTGTCTCACTAGCTGCAGGCGCTGTGTTCCTTAGTGTCATCTATCTGACATACACAGGTTATA TTGCACCATGGAGTGGCAGGTTTTATTCACTATGGGATACTGGGTATGCAAAAATACACATTCCAATTAT TGCATCAGTGTCTGAACATCAGCCTACGACATGGGTGTCTTTCTTCTTTGATCTACATATTCTTGTATGT ACCTTCCCAGCAGGCCTATGGTTCTGCATCAAAAATATCAACGATGAAAGAGTATTTGTCGCTCTGTATG CGATCAGTGCTGTCTACTTTGCCGGAGTGATGGTGCGGCTGATGCTGACTCTGACCCCGGTCGTCTGCAT GCTGTCGGCCATCGCCTTCTCCAATGTTTTTGAGCACTATTTGGGGGATGACATGAAAAGGGAAAACCCA CCTGTGGAGGACAGCAGTGATGAGGATGACAAAAGAAACCCAGGAAACTTGTATGACAAGGCAGGTAAAG TGAGGAAGCATGTGACAGAGCAAGAGAAACCTGAAGAGGGCTTGGGCCCCAACATCAAAAGCATTGTGAC CATGCTGATGCTCATGCTCCTGATGATGTTCGCGGTCCACTGCACGTGGGTCACAAGCAACGCCTACTCC AGTCCAAGTGTGGTCCTTGCCTCCTACAATCATGATGGTACCAGGAATATATTAGATGATTTTAGAGAAG CGTACTTTTGGCTGAGACAAAACACGGATGAACACGCCCGGGTCATGTCGTGGTGGGACTACGGCTATCA GATTGCTGGCATGGCCAACAGGACCACTCTGGTGGATAACAACACCTGGAACAACAGCCACATCGCACTG GTCGGAAAAGCTATGTCTTCCAATGAAACGGCCGCCTATAAAATCATGAGGTCCCTTGATGTCGATTATG TGTTGGTTATTTTCGGAGGAGTGATTGGCTATTCCGGGGACGATATCAACAAGTTCCTCTGGATGGTCAG GATAGCTGAAGGGGAGCATCCCAAAGACATCCGGGAAGGTGACTATTTCACCCAGCAGGGAGAGTTCCGA GTAGACAAAGCTGGGTCTCCTACTCTGTTAAACTGCCTTATGTATAAAATGTCATACTACAGATTTGGAG AAATGCAGCTAGATTTTCGCACTCCCCCAGGCTTTGACCGAACACGTAATGCTGAGATTGGAAATAAAGA CATTAAATTCAAGCATTTGGAGGAAGCTTTTACATCAGAGCACTGGCTTGTCAGGATATATAAAGTGAAA GCACCTGACAACAGGGAGACACTAGGTCACAAACCTCGAGTCACCAACATCGTCCCCAAACAGAAGTATT TGTCAAAGAAGACTACTAAAAGGAAGCGTGGCTACGTTAAAAATAAGCTAGTGTTTAAGAAAGGCAAGAA GACCTCTAAGAAGACTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210829 representing NM_024222
Red=Cloning site Green=Tags(s) MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGTQSASRAAAPKPGPPAGLSGGLSQPAGWQSLLS FTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVY PGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYIS RSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLM QRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLF FLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVC TFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENP PVEDSSDEDDKRNPGNLYDKAGKVRKHVTEQEKPEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYS SPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIAL VGKAMSSNETAAYKIMRSLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIREGDYFTQQGEFR VDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVK APDNRETLGHKPRVTNIVPKQKYLSKKTTKRKRGYVKNKLVFKKGKKTSKKTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024222 |
| ORF Size | 2469 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024222.3 |
| RefSeq Size | 4236 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 68292 |
| UniProt ID | Q3TDQ1 |
| Gene Summary | Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221873 | Stt3b (untagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b), (10ug) |
CNY 10,550.00 |
|
| MR210829 | Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 6,032.00 |
|
| MR210829L3 | Lenti ORF clone of Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 9,120.00 |
|
| MR210829L4 | Lenti ORF clone of Stt3b (mGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 9,120.00 |
