Odf2 (BC057001) Mouse Tagged ORF Clone

CAT#: MG210835

  • TrueORF®

Odf2 (tGFP-tagged) - Mouse outer dense fiber of sperm tails 2 (cDNA clone MGC:66991 IMAGE:6827260)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 8,248.00

CNY 9,120.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Goat Anti-ODF2 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI848335; MMTEST29
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210835 representing BC057001
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGACCGATCTTCAACTCCCCCCTTACATGTTCACGTGGATGAGAACACTCCTGTCCACGTCCACA
TAAAAAAACTCCCGAAACCGTCAGCAGCCAGCAGCCAGAAATCTCATAAGCGCGGAATGAAAGGGGACAC
CGTGAATGTACGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCTGGAAAATCATCTGCC
CGGCATGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACACCACCATCTTCAGAAAAGC
TGGTCTCGGTGATGCGATTAAGTGACCTTTCTACAGAAGACGACGATTCAGGTCACTGTAAAATGAACCG
TTACGATAAGAAGATTGACAGTCTAATGAACGCGGTCGGTTGTCTCAAATCTGAGGTCAAGATGCAGAAA
GGTGAACGCCAAATGGCCAAAAGGTTCCTGGAGGAGAGGAAGGAAGAGCTGGAGGAGGTGGCACACGAGC
TTGCAGAGACGGAGCATGAGAACACGGTGCTCAGACACAACATCGAGCGTATCAAGGAGGAGAAGGACTT
CACCATGCTTCAAAAGAAACACCTCCAGCAGGAGAAGGAATGCCTCATGTCCAAACTAGTGGAGGCTGAA
ATGGATGGAGCAGCTGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACCGAGA
AACAGATGACTTGCACCGACATCAACACCTTAACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGTAC
CTTTGAGGAGACCAATCGCACCCTCCGAGATCTGTTGAGGGAACAGCACTGCAAAGAGGATTCCGAGAGA
CTAATGGAGCAACAAGGTACATTACTGAAGCGTCTGGCAGAGGCAGACTCAGAGAAAGCGCGCCTGCTGT
TACTACTGCAAGACAAGGACAAGGAGGTGGAAGAACTCCTCCAGGAGATACAATGTGAAAAGGCTCAAGC
AAAGACAGCGTCTGAGCTTTCCAAGTCCATGGAGTCCATGCGGGGGCATTGGCAGGCACAGCTTCGCTGC
AAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATCAAGAACTTAGAGCGCAGTGGGAACCAGCATAAGG
CGGAAGTAGAGGCCATCATGGAGCAGCTAAAGGAACTGAAGCAGAAGGGAGACCGAGACAAAGAGACGCT
AAAGAAGGCCATCCGAGCCCAGAAAGAGCGAGCCGAGAAGAGTGAGGAGTATGCCGAGCAGCTGCATGTG
CAACTGGCCGACAAGGACCTTTATGTTGCTGAAGCTTTATCTACTCTGGAGTCTTGGAGGAGCCGTTACA
ACCAGGTTGTGAAGGACAAAGGAGATCTTGAGTTGGAGATTATCGTCCTAAATGACCGGGTGACAGATCT
TGTAAACCAACAACAAAGCTTGGAGGAGAAGATGCGGGAAGACCGGGACAGCTTGGTAGAGAGGCTGCAC
CGGCAGACGGCTGAGTACTCTGCATTCAAGCTAGAGAACGAGAGGCTCAAGGCTAGCTTTGCCCCTATGG
AGGACAAGCTCAACCAGGCTCACTTGGAGGTTCAGCAGCTGAAGGCGTCAGTGAAGAACTACGAGGGGAT
GATAGACAACTACAAGAGCCAGGTGATGAAAACAAGATTGGAGGCTGATGAAGTGGCTGCACAGCTAGAG
CGCTGCGACAAAGAGAACAAGATGCTTAAAGACGAGATGAACAAAGAGATCGAGGCGGCCCGTCGGCAGT
TCCAGTCACAGCTGGCTGACCTACAACAGCTGCCTGACATCCTCAAGATCACGGAGGCCAAGCTGGCTGA
GTGCCAAGACCAGCTGCAGGGCTACGAGAGGAAGAACATCGACCTCACAGCCATCATTTCAGACCTGCGC
AGCCGGATCGAACATCAGGGGGACAAGCTGGAGATGGCGAGAGAGAAACATCAGGCTTCCCAGAAGGAAA
ATAAACAGCTGAGTCAGAAGGTGGATGAACTTGAGAGGAAACTGGAGGCTACCAGCGCCCAGAATGTCGA
GTTCCTACAGGTGATTGCCAAGAGGGAGGAGGCAATCCACCAGGCTCAGCTGCGGTTGGAGGAGAAAACG
AGGGAGTGTGGGTCATTGGCGAGGCAGTTGGAGAGTGCCATTGAAGATGCCAGGCGACAGGTGGAGCAAA
CCAAGGAACAGGCACTCTCCAAGGAACGAGCAGCCCAGAGCAAAATCCTGGACCTTGAGACTCAGCTGAG
CAGGACCAAGACAGAACTTGGCCAGCTGCGGCGGACTCGTGATGATGCGGATCGCAGATACCAGAGCCGC
CTGCAGGATCTGAAAGATCGCCTGGAGCAGTCTGAGAGTACCAACCGCAGCATGCAGAATTATGTCCAAT
TCCTCAAGGCCTCTTATGCAAATGTGTTTGGGGATGCTCCCTATACATCCTCCTACCTGACGAGCTCACC
CATCCGCTCCCGATCGCCCCCTGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210835 representing BC057001
Red=Cloning site Green=Tags(s)

MKDRSSTPPLHVHVDENTPVHVHIKKLPKPSAASSQKSHKRGMKGDTVNVRRSVRVKTKVPWMPPGKSSA
RHVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQK
GERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERIKEEKDFTMLQKKHLQQEKECLMSKLVEAE
MDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSER
LMEQQGTLLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHWQAQLRC
KEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKETLKKAIRAQKERAEKSEEYAEQLHV
QLADKDLYVAEALSTLESWRSRYNQVVKDKGDLELEIIVLNDRVTDLVNQQQSLEEKMREDRDSLVERLH
RQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLE
RCDKENKMLKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLR
SRIEHQGDKLEMAREKHQASQKENKQLSQKVDELERKLEATSAQNVEFLQVIAKREEAIHQAQLRLEEKT
RECGSLARQLESAIEDARRQVEQTKEQALSKERAAQSKILDLETQLSRTKTELGQLRRTRDDADRRYQSR
LQDLKDRLEQSESTNRSMQNYVQFLKASYANVFGDAPYTSSYLTSSPIRSRSPPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057001
ORF Size 2475 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057001, AAH57001
RefSeq Size 3976 bp
RefSeq ORF 2477 bp
Locus ID 18286
Gene Summary Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...