Odf2 (BC057001) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI848335; MMTEST29
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210835 representing BC057001 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACCGATCTTCAACTCCCCCCTTACATGTTCACGTGGATGAGAACACTCCTGTCCACGTCCACA TAAAAAAACTCCCGAAACCGTCAGCAGCCAGCAGCCAGAAATCTCATAAGCGCGGAATGAAAGGGGACAC CGTGAATGTACGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCTGGAAAATCATCTGCC CGGCATGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACACCACCATCTTCAGAAAAGC TGGTCTCGGTGATGCGATTAAGTGACCTTTCTACAGAAGACGACGATTCAGGTCACTGTAAAATGAACCG TTACGATAAGAAGATTGACAGTCTAATGAACGCGGTCGGTTGTCTCAAATCTGAGGTCAAGATGCAGAAA GGTGAACGCCAAATGGCCAAAAGGTTCCTGGAGGAGAGGAAGGAAGAGCTGGAGGAGGTGGCACACGAGC TTGCAGAGACGGAGCATGAGAACACGGTGCTCAGACACAACATCGAGCGTATCAAGGAGGAGAAGGACTT CACCATGCTTCAAAAGAAACACCTCCAGCAGGAGAAGGAATGCCTCATGTCCAAACTAGTGGAGGCTGAA ATGGATGGAGCAGCTGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACCGAGA AACAGATGACTTGCACCGACATCAACACCTTAACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGTAC CTTTGAGGAGACCAATCGCACCCTCCGAGATCTGTTGAGGGAACAGCACTGCAAAGAGGATTCCGAGAGA CTAATGGAGCAACAAGGTACATTACTGAAGCGTCTGGCAGAGGCAGACTCAGAGAAAGCGCGCCTGCTGT TACTACTGCAAGACAAGGACAAGGAGGTGGAAGAACTCCTCCAGGAGATACAATGTGAAAAGGCTCAAGC AAAGACAGCGTCTGAGCTTTCCAAGTCCATGGAGTCCATGCGGGGGCATTGGCAGGCACAGCTTCGCTGC AAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATCAAGAACTTAGAGCGCAGTGGGAACCAGCATAAGG CGGAAGTAGAGGCCATCATGGAGCAGCTAAAGGAACTGAAGCAGAAGGGAGACCGAGACAAAGAGACGCT AAAGAAGGCCATCCGAGCCCAGAAAGAGCGAGCCGAGAAGAGTGAGGAGTATGCCGAGCAGCTGCATGTG CAACTGGCCGACAAGGACCTTTATGTTGCTGAAGCTTTATCTACTCTGGAGTCTTGGAGGAGCCGTTACA ACCAGGTTGTGAAGGACAAAGGAGATCTTGAGTTGGAGATTATCGTCCTAAATGACCGGGTGACAGATCT TGTAAACCAACAACAAAGCTTGGAGGAGAAGATGCGGGAAGACCGGGACAGCTTGGTAGAGAGGCTGCAC CGGCAGACGGCTGAGTACTCTGCATTCAAGCTAGAGAACGAGAGGCTCAAGGCTAGCTTTGCCCCTATGG AGGACAAGCTCAACCAGGCTCACTTGGAGGTTCAGCAGCTGAAGGCGTCAGTGAAGAACTACGAGGGGAT GATAGACAACTACAAGAGCCAGGTGATGAAAACAAGATTGGAGGCTGATGAAGTGGCTGCACAGCTAGAG CGCTGCGACAAAGAGAACAAGATGCTTAAAGACGAGATGAACAAAGAGATCGAGGCGGCCCGTCGGCAGT TCCAGTCACAGCTGGCTGACCTACAACAGCTGCCTGACATCCTCAAGATCACGGAGGCCAAGCTGGCTGA GTGCCAAGACCAGCTGCAGGGCTACGAGAGGAAGAACATCGACCTCACAGCCATCATTTCAGACCTGCGC AGCCGGATCGAACATCAGGGGGACAAGCTGGAGATGGCGAGAGAGAAACATCAGGCTTCCCAGAAGGAAA ATAAACAGCTGAGTCAGAAGGTGGATGAACTTGAGAGGAAACTGGAGGCTACCAGCGCCCAGAATGTCGA GTTCCTACAGGTGATTGCCAAGAGGGAGGAGGCAATCCACCAGGCTCAGCTGCGGTTGGAGGAGAAAACG AGGGAGTGTGGGTCATTGGCGAGGCAGTTGGAGAGTGCCATTGAAGATGCCAGGCGACAGGTGGAGCAAA CCAAGGAACAGGCACTCTCCAAGGAACGAGCAGCCCAGAGCAAAATCCTGGACCTTGAGACTCAGCTGAG CAGGACCAAGACAGAACTTGGCCAGCTGCGGCGGACTCGTGATGATGCGGATCGCAGATACCAGAGCCGC CTGCAGGATCTGAAAGATCGCCTGGAGCAGTCTGAGAGTACCAACCGCAGCATGCAGAATTATGTCCAAT TCCTCAAGGCCTCTTATGCAAATGTGTTTGGGGATGCTCCCTATACATCCTCCTACCTGACGAGCTCACC CATCCGCTCCCGATCGCCCCCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210835 representing BC057001 Red=Cloning site Green=Tags(s) MKDRSSTPPLHVHVDENTPVHVHIKKLPKPSAASSQKSHKRGMKGDTVNVRRSVRVKTKVPWMPPGKSSA RHVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQK GERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERIKEEKDFTMLQKKHLQQEKECLMSKLVEAE MDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSER LMEQQGTLLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHWQAQLRC KEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKETLKKAIRAQKERAEKSEEYAEQLHV QLADKDLYVAEALSTLESWRSRYNQVVKDKGDLELEIIVLNDRVTDLVNQQQSLEEKMREDRDSLVERLH RQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLE RCDKENKMLKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLR SRIEHQGDKLEMAREKHQASQKENKQLSQKVDELERKLEATSAQNVEFLQVIAKREEAIHQAQLRLEEKT RECGSLARQLESAIEDARRQVEQTKEQALSKERAAQSKILDLETQLSRTKTELGQLRRTRDDADRRYQSR LQDLKDRLEQSESTNRSMQNYVQFLKASYANVFGDAPYTSSYLTSSPIRSRSPPA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057001
ORF Size	2475 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057001, AAH57001
RefSeq Size	3976 bp
RefSeq ORF	2477 bp
Locus ID	18286
Gene Summary	Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly (By similarity).[UniProtKB/Swiss-Prot Function]

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