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Cars (NM_013742) Mouse Tagged ORF Clone
CAT＃: MG210862
- TrueORF®
Cars (tGFP-tagged) - Mouse cysteinyl-tRNA synthetase (Cars)
ORF Plasmid: DDK
"NM_013742" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CA3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210862 representing NM_013742
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTTCCTCCGCGGAGCAGGCTGCCGACTACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCA GGGTGCAGGCCCTGGACCAACACCTCAGCACGCGTAGCTATATCCAGGGGTACTCCCTATCCCAGGCAGA TGTGGACGTGTTCAGGCAGCTCTCGGCCCCTCCCGCTGACTCAAGGCTCTTCCACGTGGCTCGGTGGTTC AGGCACATAGAAGCGCTCCTGGGTGGCCCCCAAGGCAGAGACGAGCCCTGCAGGCTTCAAGCAAGTAAAG GCCGGCGAGTGCAGCCTCAGTGGTCCCCCCCAGCAGGGACTGAGCCCTGCAGGCTCCGCCTCTATAATAG CCTCACCCGGAACAAGGATGTATTTATACCTCAAGATGGGAAGAAGGTGACGTGGTACTGTTGCGGGCCG ACTGTCTATGATGCGTCTCACATGGGACATGCCAGGTCCTACATCTCCTTCGATATCCTGAGGAGGGTGC TGAGGGATTACTTCCAGTATGATGTCTTTTACTGCATGAACATCACAGACATTGATGATAAGATCATCAG GCGGGCACGGCAGAACTACCTGTTTGAGCAGTATCGGGAGCAGAAACCCCCAGCCACCCAGCTCCTGAAG GATGTCCGTGATGCCATGAAGCCATTTTCAGTCAAGTTAAGTGAGACAACAGATCCCGACAAGAGGCAGA TGCTGGAGCGGATCCAGAACTCTGTGAAACTTGCCACAGAACCACTGGAACAGGCTGTGCGATCCAGCCT CTCTGGAGAGGAAGTAGACAGCAAAGTACAGGTGTTGCTGGAGGAAGCAAAGGACTTGCTCTCTGACTGG CTGGATTCCACAGGTGGCAGTGAGGTGACTGACAACTCGATTTTCTCCAAACTGCCCAAGTTCTGGGAAG AAGAGTTCCACAAAGACATGGAAGCCCTGAATGTTCTCCCTCCTGATGTCCTAACCCGTGTCAGTGAATA TGTGCCAGAAATTGTGAACTTCGTCCAGAAGATTGTGGACAATGGTTATGGTTATGCTTCAAATGGATCT GTTTACTTCGACACAGCCAAGTTTGCTGCTAGTGAAAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCG TTGGGGATCAGAAGGCACTTCAGGAAGGGGAAGGTGATCTGAGCATCTCTGCTGACCGCCTTAGTGAGAA ACGCTCTCCCAATGACTTTGCCTTGTGGAAGGCCTCCAAGCCAGGCGAGCCATCCTGGCCTTGCCCCTGG GGAAAGGGTCGTCCCGGATGGCACATAGAGTGTTCTGCTATGGCAGGCACGCTCCTGGGAGCCTCAATGG ACATTCATGGTGGAGGGTTTGACCTCCGCTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTA CTTTGAAAATGACTGCTGGGTCAGGTACTTCTTGCACACGGGCCACTTGACGATAGCAGGCTGCAAGATG TCCAAGTCACTGAAAAACTTTATCACCATTAAAGATGCCTTGAAGAAGCACTCAGCACGGCAGCTGCGAC TGGCATTCCTCATGCACTCATGGAAAGACACACTGGACTATTCCAGCAACACTATGGAGTCTGCTCTTCA GTATGAAAAATTCATGAACGAGTTTTTCTTAAATGTGAAAGACATCCTCCGAGCCCCTGTAGACATCACT GGCCAGTTTGAAAAGTGGGAAGCTGAAGAAGTGGAGCTAAATAAGAACTTCTATGGCAAGAAGACCGCAG TTCACGAAGCCTTGTGTGATAACATTGACACCCGGACTGTCATGGAAGAGATGCGGGCTTTGGTCAGTCA GTGCAACCTCTACATGGCAGCCAGGAAGGCTGAACGGAGGAGGCCCAACCGGGCTCTGCTGGAGAACATT GCCATGTACCTCACCCACATGCTGAAGATCTTTGGGGCCATAGAGGAGGAGAGCCCCCTGGGGTTCCCAG TTGGTGGGCCTGGAACCAACCTGAACCTCGAGTCAACAGTCATGCCCTACCTTCAGGTGTTATCAGAATT CAGAGAGGGAGTACGAAAGATTGCCCGAGAGAAAAAAGTTCTTGAGGTTCTACAGCTCAGTGATGCCCTC CGGGATGACATCCTGCCTGAGCTTGGGGTCCGGTTTGAAGACCATGAAGGGCTGCCAACAGTGGTGAAGT TGGTGGACAGAGACACCTTACTGAAAGAGAAGGAAGGAAAGAAAAGGGCTGAAGAAGAGAAGAGGAGGAA GAAAGAGGAGGCGGCCAGAAAGAAACAGGAGCAAGAAGCAGCAAAGCTGGCCAAGATGAAGATACCACCC AGTGAGATGTTCCTGTCAGAAGTCAACAAGTATTCTAAATTTGATGAAAATGGTCTGCCTACCCACGACA CCGAAGGCAAAGAGCTGAGCAAGGGCCAGGCCAAGAAGCTGAAGAAGCTCTTTGAAGCCCAGGAGAAACT GTACAAGGAGTATCTGCAAATGTTACAGAATGGCAGCCTCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210862 representing NM_013742
Red=Cloning site Green=Tags(s) MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLSQADVDVFRQLSAPPADSRLFHVARWF RHIEALLGGPQGRDEPCRLQASKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGP TVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLK DVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDW LDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGS VYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPW GKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKM SKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDIT GQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENI AMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDAL RDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPP SEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013742 |
| ORF Size | 2493 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013742.5, NP_038770.3 |
| RefSeq Size | 3236 bp |
| RefSeq ORF | 2496 bp |
| Locus ID | 27267 |
| UniProt ID | Q9ER72 |
| Gene Summary | Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205092 | Cars (untagged) - Mouse cysteinyl-tRNA synthetase (Cars), (10ug) |
CNY 6,096.00 |
|
| MR210862 | Cars (Myc-DDK-tagged) - Mouse cysteinyl-tRNA synthetase (Cars) |
CNY 6,088.00 |
|
| MR210862L3 | Lenti ORF clone of Cars (Myc-DDK-tagged) - Mouse cysteinyl-tRNA synthetase (Cars) |
CNY 7,700.00 |
|
| MR210862L4 | Lenti ORF clone of Cars (mGFP-tagged) - Mouse cysteinyl-tRNA synthetase (Cars) |
CNY 7,700.00 |
