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Dnm1 (BC034679) Mouse Tagged ORF Clone
CAT＃: MG211008
- TrueORF®
Dnm1 (tGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865)
ORF Plasmid: DDK
"BC034679" in other vectors (4)
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CNY 7,952.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI838169; Dnm; Ftfl; mKIAA4093 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211008 representing BC034679
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATCG GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCGGT GCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTGGTC CTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACCGACT TCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATTTCGCC AGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCAGGAATG ACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATGCAGTTCG TCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCTGATGCCCT CAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTGGACCTGATG GACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGTTACATCGGCG TGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCTGCAGAGCGCAA ATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTACCTGCAGAAGGTC CTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAGCTGCAGAGCCAGC TGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCAGCGCGCAAGACCAA GGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAGGGTTCTGGAGACCAG ATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAACGCTTCCCCTTTGAGC TGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCTATCAAAAATATCCATGG CATCAGAACGGGGCTCTTTACCCCAGACATGGCCTTTGAAACCATTGTGAAAAAGCAGGTGAAGAAGATT CGAGAGCCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGCACGGTTAGACAGTGCACCA AGAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTGACCACCCACATCCGGGAACG TGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTGGCTTACATGAATACCAACCAC GAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA ACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAACAACATCGGCATCATGAAGGGAGG CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG AAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTGGAGAAGGGCTTCATGTCAAGCAAGC ATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAGGATTACCGGCAGCTGGAACTGGCCTG TGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT GGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCTGACAGCTTCATGCACTCGATGGACCCTC AGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGACTCGTACATGGCCATTGTCAACAAGACTGT GCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATCAACAACACCAAGGAGTTTATCTTCTCTGAG CTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGC GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACAC GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC GGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG GATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTC CAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCG CCTGGGGTCCCCAGCCTAGGAGCATGGCGACTAAATTCACCACAGGGCAAGCATGAGAATCGCGCTGGCA AAGCCAGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211008 representing BC034679
Red=Cloning site Green=Tags(s) MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV LQLVNSTTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKLDLM DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV LNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVDEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKI REPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH EDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKE KKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERV GDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSE LLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPA GRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAP PGVPSLGAWRLNSPQGKHENRAGKARL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034679 |
| ORF Size | 2601 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034679, AAH34679 |
| RefSeq Size | 3448 bp |
| RefSeq ORF | 2603 bp |
| Locus ID | 13429 |
| Gene Summary | This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220643 | Dnm1 (untagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865), (10ug) |
CNY 9,120.00 |
|
| MR211008 | Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 6,352.00 |
|
| MR211008L3 | Lenti ORF clone of Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 6,840.00 |
|
| MR211008L4 | Lenti ORF clone of Dnm1 (mGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 6,840.00 |
