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Dnm1 (BC034679) Mouse Tagged ORF Clone
CAT＃: MR211008
- TrueORF®
Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865)
ORF Plasmid: tGFP
"BC034679" in other vectors (4)
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CNY 6,352.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI838169; Dnm; Ftfl; mKIAA4093 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211008 representing BC034679.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATC GGCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCG GTGCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTG GTCCTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACC GACTTCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATT TCGCCAGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCA GGAATGACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATG CAGTTCGTCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCT GATGCCCTCAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTG GACCTGATGGACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGT TACATCGGCGTGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCT GCAGAGCGCAAATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTAC CTGCAGAAGGTCCTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAG CTGCAGAGCCAGCTGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCA GCGCGCAAGACCAAGGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAG GGTTCTGGAGACCAGATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAA CGCTTCCCCTTTGAGCTGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCT ATCAAAAATATCCATGGCATCAGAACGGGGCTCTTTACCCCAGACATGGCCTTTGAAACCATTGTGAAA AAGCAGGTGAAGAAGATTCGAGAGCCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGC ACGGTTAGACAGTGCACCAAGAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTG ACCACCCACATCCGGGAACGTGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTG GCTTACATGAATACCAACCACGAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATG AACAAGAAGAAGACTTCAGGGAACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAAC AACATCGGCATCATGAAGGGAGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGG TACAAGGATGATGAGGAGAAAGAGAAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTG GAGAAGGGCTTCATGTCAAGCAAGCATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAG GATTACCGGCAGCTGGAACTGGCCTGTGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTG AGGGCTGGCGTGTACCCTGAGCGTGTTGGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCT GACAGCTTCATGCACTCGATGGACCCTCAGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGAC TCGTACATGGCCATTGTCAACAAGACTGTGCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATC AACAACACCAAGGAGTTTATCTTCTCTGAGCTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACA CTGATGGAAGAATCGGCCGAGCAGGCTCAGCGGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAG GAGGCGCTCAGTATTATCGGCGACATCAACACGACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGAC GACTCCTGGCTGCAGGTGCAGAGCGTACCGGCCGGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAG CGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCGGATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCT GGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCC CCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCTGGGGTCCCCAGCCTAGGAGCATGGCGACTA AATTCACCACAGGGCAAGCATGAGAATCGCGCTGGCAAAGCCAGGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by MR211008
Blue=ORF Red=Cloning site Green=Tag(s) MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPL VLQLVNSTTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLP GMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKL DLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPY LQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVDEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIE GSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVK KQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIEL AYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSW YKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFL RAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMI NNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVD DSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGP PPQVPSRPNRAPPGVPSLGAWRLNSPQGKHENRAGKARL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using MR211008 also available, TP511008 |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034679 |
| ORF Size | 2601 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 3448 bp |
| RefSeq ORF | 2603 bp |
| Locus ID | 13429 |
| MW | 97.8 kDa |
| Gene Summary | This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220643 | Dnm1 (untagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865), (10ug) |
CNY 9,120.00 |
|
| MG211008 | Dnm1 (tGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 7,952.00 |
|
| MR211008L3 | Lenti ORF clone of Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 6,840.00 |
|
| MR211008L4 | Lenti ORF clone of Dnm1 (mGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) |
CNY 6,840.00 |
