Cyfip2 (BC056974) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1500004I01Rik; 6430511D02Rik; AA930218; AU022376; mKIAA1168; Pir121
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211086 representing BC056974 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCCACGTCACTTTGGAAGATGCCCTGTCCAACGTGGACCTGCTGGAGGAACTGCCCCTCCCAG ACCAGCAGCCATGTATCGAGCCCCCGCCATCCTCCATCATGTACCAGGCTAACTTTGACACGAACTTCGA GGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATTGAGCAGGCAACGGTCCACTCCAGCATGAAT GAAATGCTGGAGGAGGGCCACGACTATGCAGTCATGCTGTACACCTGGCGCAGCTGCTCCCGGGCCATCC CGCAGGTGAAGTGCAATGAGCAGCCCAATCGGGTCGAGATCTACGAGAAGACGGTGGAGGTGCTGGAGCC AGAGGTCACCAAGCTCATGAAGTTCATGTACTTTCAGCGCAAGGCCATCGAGCGCTTCTGTAGCGAGGTG AAGCGGCTGTGTCACGCTGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTGCTGACGCTGGGCAAGT TCATCAACATGTTCGCGGTCTTGGACGAGCTAAAGAACATGAAGTGCAGTGTCAAGAATGACCACTCTGC CTACAAGAGAGCAGCACAGTTCCTAAGGAAGATGGCGGACCCCCAGTCCATCCAGGAATCTCAGAACCTG TCCATGTTCCTGGCCAATCACAACAGGATCACCCAGTGTCTTCACCAGCAACTGGAAGTGATCCCGGGCT ATGAGGAGCTGCTGGCAGACATTGTCAACATCTGTGTTGATTACTATGAGAATAAGATGTACCTGACGCC CAGTGAAAAGCACATGTTGCTCAAGGTGATGGGCTTTGGTCTCTATCTGATGGACGGAAATGTCAGTAAC ATTTACAAACTGGATGCCAAGAAGAGAATCAACCTTAGCAAAATTGATAAGTTTTTTAAGCAGCTGCAGG TGGTACCCCCTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAGACCAGTGCTCACTATGAAGA GAACAAGTCCAAGTGGACATGCACTCAGAGCAGCATCAGCCCCCAGTACAACATCTGTGAGCAGATGGTT CAGATCCGGGATGATCACATCCGCTTCATCTCTGAGCTGGCTCGCTACAGCAACAGCGAGGTGGTGACAG GCTCAGGGCTGGACAGCCAGAAGTCGGATGAAGAGTACCGGGAGCTTTTTGACCTGGCCCTGCGAGGCCT GCAGCTTCTATCCAAGTGGAGTGCCCATGTCATGGAGGTGTACTCTTGGAAGCTGGTTCACCCCACAGAC AAGTTCTGCAACAAGGACTGCCCGGGCACCGCGGAAGAGTACGAGAGAGCCACGCGCTACAATTATACCA GCGAGGAGAAGTTTGCCTTTGTGGAGGTGATTGCCATGATCAAGGGTTTGCAGGTGCTCATGGGAAGGAT GGAGAGTGTCTTCAACCAGGCCATCCGCAACACTATCTACGCAGCCCTGCAGGATTTCGCCCAGGTGACG CTGCGGGAGCCGTTGCGGCAAGCAGTTCGAAAGAAAAAGAATGTCCTTATCAGCGTTCTGCAAGCAATTC GAAAGACCATCTGTGACTGGGAGGGAGGCCGAGAGCCCCCCAATGACCCATGCTTAAGAGGGGAGAAGGA CCCCAAAGGTGGCTTTGACATCAAGGTGCCCCGCCGTGCTGTGGGTCCATCCAGCACACAGCTGTACATG GTGCGCACCATGCTTGAGTCACTCATCGCAGACAAGAGCGGCTCCAAGAAGACGCTCAGAAGCAGCCTGG ATGGCCCTATCGTCCTTGCCATAGAGGACTTCCACAAGCAGTCCTTCTTTTTCACACACCTGCTTAACAT CAGCGAGGCTCTTCAGCAGTGCTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGTTGACC ATGGGCCGGCGAATCCAGTTCCCGATTGAGATGTCCATGCCCTGGATTCTAACGGACCATATTCTGGAAA CCAAAGAACCTTCCATGATGGAGTATGTCCTCTACCCCTTGGATCTGTACAACGACAGCGCCTACTATGC CCTCACCAAATTCAAAAAGCAGTTCCTGTATGACGAGATCGAAGCCGAGGTAAACCTGTGTTTTGATCAG TTTGTCTACAAGCTGGCTGACCAGATCTTCGCCTACTACAAAGCCATGGCCGGCAGTGTCCTGCTGGATA AACGTTTTAGAGCTGAGTGTAAGAACTATGGGGTGATCATACCGTACCCGCCATCCAACCGCTACGAGAC ACTGCTGAAGCAGAGACATGTCCAGCTGTTGGGGAGATCAATTGACTTGAACAGACTCATCACCCAGCGG ATCTCTGCTGCCATGTACAAGTCCTTGGACCAGGCCATCAGCCGCTTTGAGAGTGAAGACCTGACCTCCA TTGTGGAGCTGGAGTGGCTGCTGGAGATTAACCGACTCACACACCGACTCCTCTGTAAGCACATGACGCT AGACAGCTTCGATGCCATGTTCCGAGAAGCCAATCATAACGTGTCTGCCCCTTACGGCCGCATCACCCTG CATGTCTTCTGGGAGCTGAACTTTGACTTTCTCCCCAACTACTGCTATAATGGATCCACAAACCGTTTTG TCCGAACTGCCATCCCTTTCACCCAAGAACCACAACGGGATAAGCCCGCCAATGTCCAGCCTTATTACCT CTATGGATCCAAGGCCTCCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211086 representing BC056974 Red=Cloning site Green=Tags(s) MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMN EMLEEGHDYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEV KRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNL SMFLANHNRITQCLHQQLEVIPGYEELLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSN IYKLDAKKRINLSKIDKFFKQLQVVPPFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMV QIRDDHIRFISELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTD KFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVT LREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKGGFDIKVPRRAVGPSSTQLYM VRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELT MGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQ FVYKLADQIFAYYKAMAGSVLLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQR ISAAMYKSLDQAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITL HVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKASK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC056974
ORF Size	2684 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC056974, AAH56974
RefSeq Size	4452 bp
RefSeq ORF	2684 bp
Locus ID	76884
Gene Summary	Part of the WAVE1 complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). Involved in T-cell adhesion and p53-dependent induction of apoptosis (By similarity). Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function]

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