Zfp62 (BC065692) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211138 representing BC065692 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAGGAGTCGTCTAAAAAGAATGAAAATGATTCAAATGCAGACTCTCAGTGCCCAAGTGTGG GATTTTTTCATAAGGATCATATGCAGAAATCTAAGACCGGTGACACTTGTGACTTGTTTCCTAAATGGAA AATACTGAAGGAAGGCAAAAGTAGTATCAGAGAAATGATTGACACACATACAAACGCAGCAAATATAAAA CTAGAGCAGGATGATGAGACATCTGAGAAAAGTTTCTACCCAAGTACAAATACAATGCACCAGACTATCC CCACAGAACCAAATTGTACTAAACAAGGTGAGCACACAGAGAACATTAATGGAAACGTACACCCTGCTCA TATTGCTGATAAGAAATTACATAAGTGTGATGAGTGTGGAAAATCCTTCAAATACAATTCTCGTCTTGTT CAACATAAAATTATGCACACTGGAGAAAAGCGCTACGAGTGTGATGACTGTAGGGGGACTTTTCGGAGTA GCTCCAGTCTTCGAGTCCACAAGCGCATCCATACTGGGGAAAAGCCCTATAAGTGTGATGAGTGTGGCAA AGCCTACATGTCCTACTCTAGCCTTATAAACCACAAAAGCACTCATTCTGGGGAGAAGAACTGCAAATGT GACGAATGTGGAAAATCCTTCAATTATAGTTCTGTTCTGGACCAGCATAAAAGGATCCACACCGGAGAGA AGCCCTATGAATGTGGTGAGTGTGGAAAGGCCTTCAGAAACAGTTCTGGTTTGAGAGTCCACAAAAGGAT CCACACAGGGGAGAAGCCCTATGAATGTGATACCTGTGGGAAAACCTTCAGTAATAGTTCTGGCCTGAGG GTCCACAAAAGGATCCATACAGGTGAGAAACCCTATGAATGTGATGAATGTGGGAAGGCATTCATTACTT GCAGAACACTTCTAAATCATAAAAGCATCCACTTTGGTGATAAACCTTATAAATGTGATGAGTGTGAAAA ATCTTTTAATTACAGCTCTCTCCTCATTCAGCATAAAGTCATCCACACTGGTGAGAAACCTTATGAATGT GATGAATGTGGGAAGGCCTTCAGGAACAGCTCAGGCCTTATAGTGCATAAAAGGATCCACACAGGAGAGA AACCTTACAAGTGTGATATCTGTGGCAAAGCATTCAGCTATAGTTCAGGCCTTGCAGTCCATAAAAGCAT TCATCCTGGAAAGAAAGCCCATGAATGTAAAGACTGTGGCAAGTCCTTTAGTTATAACTCACTTCTTCTT CAGCATAAGACCATTCATACTGGAGAAAGACCTTATGTATGTGATGTGTGTGGCAAAACGTTCAGGAACA ATTCAGGTCTCAAAGTTCATCGGAGACTCCATACTGGGGAAAAACCATATAAGTGTGATGTATGTGGGAA GGCCTATATCTCACGCTCAAGCCTTAAAAATCACAAAGGAATCCACATGGGGGAGAAGCCCTATAAATGC AGCTATTGTGAGAAATCTTTCAACTACAGTTCTGCCCTTGAACAACATAAAAGGATTCATACAAGGGAGA AGCCCTTTGGGTGTGATGAGTGTGGAAAAGCCTTCAGAAACAATTCAGGCCTTAAAGTACATAAGCGAAT TCACACAGGGGAGAGACCTTACAAATGTGAAGAATGTGGAAAAGCCTACATCTCACTCTCAAGCCTTATA AATCATAAAAGTGTACACCCTGGGGAAAAGCCCTTTAAGTGTGATGAGTGTGAGAAAGCCTTCATCACAT ACAGAACACTTCTAAACCACAAAAAAATTCATCTTGGAGAAAAGCCCTATAAATGCGATGTGTGTGAGAA ATCTTTTAACTACACCTCACTCCTTTCTCAACACAAAAGAGTTCATACTAGAGAGAAACCCTTTGAATGT GACAGGTGTGAGAAGGTCTTCAGAAACAACTCAAGCCTTAAAGTGCATAAAAGAATACACACTGGGGAGA AGCCCTATGAGTGTGACATATGTGGAAAAGCCTACATCTCACACTCAAGCCTTATTAACCATAAAAGTAC ACACCCTGGCAAGACGTCCTATACATGTGATGAATGCGGAAAAGCATTTTTCTCAAGCAGAACTCTTATA AGCCATAAGAGAGTCCATCTTGGGGAAAAGCCCTTCAAGTGTGTTGAGTGTGGGAAGTCGTTCAGCTACA GCTCACTTCTTTCCCAACACAAGAGGATCCACACAGGGGAGAAACCTTATGTATGTGATTGGTGTGGGAA GGCATTCAGGAACAGCTCAGGCCTCACAGTACATAAAAGGATCCACACAGGAGAGAAACCCTATGGGTGT GATGAATGTGAAAAGGCATACATTTCACACTCAAGTCTTATTAATCATAAAAGTGTCCACCGTGGAAAGC AGCCCTATAATTGTGAGTGTGGGAAATCTTTCAATTACAGATCTGTCCTTGACCAACATAAAAGAATCCA CACTGGAAAAAAGCCATATCGATGTAATGATTGTGGGAAGGCATTTAATATCAGGTCAAACCTTACCAAG CACAAAAGAATCCATACAGGAGAGGAATCTTTAAATATGGCAAATATGGAAAGTCATAGTGGCACATTCC AGAAGATGATCTACTATGAGGGAGGGAATTCTCTGGATGGGACCAGAATGCAGATGCCTGTGTGGGAGGC AGAGCATGCCAAGTCTCAAAGAAATCAAATAGAAGAAAAACTTTATGAATATAACAATTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211138 representing BC065692 Red=Cloning site Green=Tags(s) MEDEESSKKNENDSNADSQCPSVGFFHKDHMQKSKTGDTCDLFPKWKILKEGKSSIREMIDTHTNAANIK LEQDDETSEKSFYPSTNTMHQTIPTEPNCTKQGEHTENINGNVHPAHIADKKLHKCDECGKSFKYNSRLV QHKIMHTGEKRYECDDCRGTFRSSSSLRVHKRIHTGEKPYKCDECGKAYMSYSSLINHKSTHSGEKNCKC DECGKSFNYSSVLDQHKRIHTGEKPYECGECGKAFRNSSGLRVHKRIHTGEKPYECDTCGKTFSNSSGLR VHKRIHTGEKPYECDECGKAFITCRTLLNHKSIHFGDKPYKCDECEKSFNYSSLLIQHKVIHTGEKPYEC DECGKAFRNSSGLIVHKRIHTGEKPYKCDICGKAFSYSSGLAVHKSIHPGKKAHECKDCGKSFSYNSLLL QHKTIHTGERPYVCDVCGKTFRNNSGLKVHRRLHTGEKPYKCDVCGKAYISRSSLKNHKGIHMGEKPYKC SYCEKSFNYSSALEQHKRIHTREKPFGCDECGKAFRNNSGLKVHKRIHTGERPYKCEECGKAYISLSSLI NHKSVHPGEKPFKCDECEKAFITYRTLLNHKKIHLGEKPYKCDVCEKSFNYTSLLSQHKRVHTREKPFEC DRCEKVFRNNSSLKVHKRIHTGEKPYECDICGKAYISHSSLINHKSTHPGKTSYTCDECGKAFFSSRTLI SHKRVHLGEKPFKCVECGKSFSYSSLLSQHKRIHTGEKPYVCDWCGKAFRNSSGLTVHKRIHTGEKPYGC DECEKAYISHSSLINHKSVHRGKQPYNCECGKSFNYRSVLDQHKRIHTGKKPYRCNDCGKAFNIRSNLTK HKRIHTGEESLNMANMESHSGTFQKMIYYEGGNSLDGTRMQMPVWEAEHAKSQRNQIEEKLYEYNNF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC065692
ORF Size	2723 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC065692, AAH65692
RefSeq Size	4130 bp
RefSeq ORF	2723 bp
Locus ID	22720
Gene Summary	May play a role in differentiating skeletal muscle.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.