Bclaf1 (BC034300) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Btf, mKIAA0164, MGC30519
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211171 representing BC034300 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGCTCCAATTCTAGATCACATTCTTCAAGATCCAAGTCTAGATCACAGTCTAGTTCTAGATCAA GATCAAGATCACATTCTAGAAAGAAGAGATACAGGTCTCGTTCCAGAACATATTCGAGGTCTCGTAGTAG AGATCGTATTTATTCTAGAGATTATCGTCGAGATTACAGGAATAATAGAGGAATGAGACGACCTTATGGG TACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGGAGGAGGGAGATACCATCGAGGTGGCTATAGACCTG TCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGGTCACGGTCCAGGAGTCCAAAAAGAAGATC CGTGTCTTCTCAAAGATCCCGAAGCAGATCTCGCCGGTCATATAGATCCTCTAGGTCTCCAAGATCATCA TCATCTCGCTCTTCATCCCCATATAGCAAATCTCCTGTCTCTAAAAGACGAGGGTCTCAGGAAAAACAAA CCAAAAAAGCTGAGGGGGAACCCCAAGAAGAGAGTCCTTTGAAAAGCAAATCACAGGAGGAACCAAAGGA TACTTTTGAACATGATCCATCTGAATCTATTGATGAGTTTAATAAATCTGCTACTTCTGGTGATATTTGG CCTGGCCTTTCAGCTTATGATAATAGTCCAAGGTCACCTCATAGTCCTTCACCTATTGCTACACCACCCA GTCAGAGTTCATCATGCTCAGATGCCCCCATGCTTAGTACAGTGCACTCTGCCAAAAATACCCCTTCTCA GCATTCACATTCCATTCAGCACAGTCCTGAGAGGTCTGGATCTGGCTCTGTTGGAAATGGGTCGAGTCGA TATAGTCCTTCTCAGAATAGTCCGATTCATCACATCCCATCACGAAGAAGCCCTGCCAAGACAATCACAC CACAGAATGCTCCAAGAGAGGAATCTAGGGGGCGGTCCTCCTTTTATCCTGAAGGAGATCAGGAAACAGC AAAGACAGGAAAGTTTTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCCTGCTTGATAGGGGTAAT ATCAGGGATAAAGAGGCTCCGAAGGAGAAAGGGTCAGAGAAAGGCAGGGCAGATGGAGATTGGGATGACC AGGAAGTTCTAGATTACTTTAGTGATAAAGAGTCTGCAAAACAAAAATTCCATGACTCTGAAGGGGATGA CACAGAGGAGACAGAGGATTATAGACAGTTTAGGAAATCAGTCCTAGCAGATCAGGGCAAGAGCTTTGCT ACTTCATCTCACCGGAATACTGAGGAGGAAGGACCCAAGTACAAGTCCAAAGTTTCATTAAAAGGCAATA GAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTGAAAGAGACTGCATACATAGTCGAAAGGCC TAGCACTGCAAAGGACAAGCACAAGGAAGAAGACAAAGGCTCTGATAGAATAACAGTGAAGAAAGAAGTG CAGTCACCGGAGCAGGTAAAGTCTGAAAAGCTCAAAGAGCTCTTTGATTACAGTCCCCCTCTACACAAGA GTCTGGATGCACGAGAAAAGTCCATCTTCAGAGAGGAGAGCCCACTGAGGATCAAAATGATAGCCAGTGA TTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCCTCTTGACGATTCCAACAGACCTGCCTCC TTGACTAAAGACAGGCTACTTGCTAGCACGCTTGTCCATTCTGTCAAGAAAGAGCAAGAATTCCGATCCA TCTTTGACCACATTAAGCTGCCTCAGGCCAACAAAAGCACTTCAGAATCATTTATACAACACATTGTATC CTTGGTTCATCATGTGAAAGAACAATACTTCAAATCACCTGCAGTGACCCTAAACGAGCGGTTCACTTCA TACCAGAAAGCTACTGAAGAACATAGTACCCGGCAAAAGAGCCCCGAGATACACAGGAGGATTGACATCT CTCCCAGCGCTCTGAGGAAGCACACCCGGCTAGCAGGAGAGGAGAGAGGTTTTAAAGAAGAAATTCAGAA GGGAGATAAAAAGTTAAGGTGTGATTCGGCTGACCTTCGGCACGACATTGACCGTCGCCGTAAAGAGAGA AGTAAAGAGCGGGGAGATTCCAAGGGCTCCAGGGAGTCCAGTGGGTCCAGAAAGCAAGAAAAAACACCAA AGGATTACAAGGAGTATAAACCTTACAAAGATGACAGCAAACATAAAGGTAGAGAGCGGGACCATTCCCG GTCATCGTCCTCGTCAGCATCGCCGTCCTCGCCCAGTTCTCGAGAAGAAAAAGAGAGTAAGAAAGAGAGA GAAGAAGAATTTAAAACTCACCACGAGATGAAAGACTACTCAGGATTTGCAGGAGTTAGCAGACCACGAG GAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAA CAACTCAAATACCACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGC AAGAAGTACTTCTTGCACGATGACAGAGATGATGGTGTGGATTACTGGGCCAAAAGAGGAAGAGGTCGTG GTACTTTTCAACGTGGCAGAGGGCGCTTTAATTTCAAAAAATCAGGTAGCAGTCCAAAATGGACTCATGA CAAATACCAAGGAGATGGGATTGTTGAAGATGACGAAGAGACCATGGAAAATAATGAAGAGAAGAAGGAC AGACGCAAAGAAGAAAAGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211171 representing BC034300 Red=Cloning site Green=Tags(s) MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYRSRSRTYSRSRSRDRIYSRDYRRDYRNNRGMRRPYG YRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSS SSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSATSGDIW PGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSR YSPSQNSPIHHIPSRRSPAKTITPQNAPREESRGRSSFYPEGDQETAKTGKFLKRFTDEESRVFLLDRGN IRDKEAPKEKGSEKGRADGDWDDQEVLDYFSDKESAKQKFHDSEGDDTEETEDYRQFRKSVLADQGKSFA TSSHRNTEEEGPKYKSKVSLKGNRESDGFREEKNYKLKETAYIVERPSTAKDKHKEEDKGSDRITVKKEV QSPEQVKSEKLKELFDYSPPLHKSLDAREKSIFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPAS LTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQANKSTSESFIQHIVSLVHHVKEQYFKSPAVTLNERFTS YQKATEEHSTRQKSPEIHRRIDISPSALRKHTRLAGEERGFKEEIQKGDKKLRCDSADLRHDIDRRRKER SKERGDSKGSRESSGSRKQEKTPKDYKEYKPYKDDSKHKGRERDHSRSSSSSASPSSPSSREEKESKKER EEEFKTHHEMKDYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKS KKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDDEETMENNEEKKD RRKEEKE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034300
ORF Size	2753 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034300, AAH34300
RefSeq Size	3157 bp
RefSeq ORF	2753 bp
Locus ID	72567
Gene Summary	Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA (By similarity).[UniProtKB/Swiss-Prot Function]

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