Mkl1 (NM_001082536) Mouse Tagged ORF Clone
CAT#: MG211216
- TrueORF®
Mkl1 (tGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2
ORF Plasmid: DDK
"NM_001082536" in other vectors (4)
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CNY 7,890.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AMKL; Bsac; Mal; Mrtf-A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211216 representing NM_001082536
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCTTTGAAAAGCCCCGCTGCATTTCATGAGCAGAGAAGAAGCCTGGAGCGGGCCAGGACCGAGG ACTATTTGAAACGGAAGATCCGTTCCCGGCCCGAGAGAGCAGAGCTGGTCAGGATGCACATTCTGGAAGA GACCTCGGCTGAGCCTTCGCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGGCTGGCTGATGACCTC AATGAAAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAATATCCTGCCTGTGGAGTCCA GCCTGAAGGAGGCTATCATTGTGGGCCAGGTAAATTACCCAAAGGTAGCAGACAGTTCCTCCTTCGACGA GGACAGCAGCGATGCCCTGTCTCCTGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCAGTGCCATCACCC TTGGAGTCCCGAGTCAGTGATCCACTGCCCAGTGCCACCTCCATATCACCCACTCAGGTTCTTTCTCAAC TCCCAATGGCTCCGGATCCTGGAGAGACGCTTTTTCTGGCAGAGCAGCCTCCTCTGCCTCCCGCACCTCT GCTGCCCCCAAGCCTAGCCAATGGAAGCATCGTCCCCACTGCCAAGCCTGCTCCCACACTCATCAAGCAA AGCCAACCCAAGTCTGCCAGCGAGAAATCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGA AGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAAGGGGGCGCCCGCCATGGACTC CTCCTATGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTGCAGCTGCAGATCCTCAACCAGCAGCAGCAG CAGCAGCAGCAACAGCACTACAACTACCAGGCCATCCTGCCTGCCCCTCCCAAGCCCTCGGCTGAGACTC CTGGAAGCAGTGCCCCTACCCCATCACGCAGCCTCTCCACCAGTAGCAGCCCCAGCTCAGGCACCCCAGG GCCCAGCGGGCTGGCACGCCAGAGCAGCACCGCACTAGCTGCCAAACCAGGAGCCCTGCCAGCCAACCTG GATGACATGAAGGTGGCAGAGCTGAAGCAAGAACTGAAGTTGCGGTCCCTTCCCGTCTCAGGCACCAAGA CAGAGCTGATAGAACGCCTGCGTGCCTACCAAGACCAAGTCAGCCCAGCTCCAGGAGCCCCCAAGGCCCC TGCCACCACCTCTGTGCTGTCCAAGGCTGGTGAGGTAGTGGTCGCCTTCCCTGCGGCCCTGCTAAGCACA GGGTCAGCTCTTGTAACAGCAGGCCTTGCACCAGCTGAGATGGTGGTGGCCACAGTAACCAGCAATGGCA TGGTGAAGTTTGGCAGCACAGGCTCCACACCCCCCGTGTCTCCCACCCCTTCAGAGCGCTCACTGCTCAG CACGGGTGATGAGAATTCCACACCTGGGGATGCCTTTGGTGAAATGGTGACATCGCCGCTGACACAGCTC ACCCTGCAGGCCTCCCCACTGCAGATCGTGAAGGAGGAGGGTGCCCGTGCTGCGTCCTGCTGTCTAAGCC CTGGTGCTCGGGCTGAGCTGGAGGGACTGGACAAGGACCAGATGCTGCAGGAGAAGGACAAGCAGATTGA GGAGCTGACCCGAATGCTCCAACAGAAGCAGCAGCTGGTTGAGCTGCTGCGGCTACAGCTGGAGCAGCAG AAGCGGGCCCAGCAGCCAGCCCCAGCCAGCAGCCCTGTGAAGAGGGAAAGTGGTTTCTCCAGTTGCCAGC TGAGCTGCCAGCCCCAGGGCTCTGCCCATGCTTTTGGCTCTGGCCTAGTGGTTCCCACTACCAACCATGG AGACACTCAGGCCCCAGCGCCAGAGTCCCCACCTGTGGTGGTGAAGCAGGAAGCTGGGCCACCTGAGCCA GATCTGGCCCCCAGCTCCCAGCTGCTCTTGGGCTCCCAGGGCACCAGCTTCCTCAAGAGGGTCAGCCCTC CTACCCTGGTCACTGACTCTACAGGGACTCACCTCATCCTCACTGTGACCAATAAGAGTGCTGATGGCCC TGGCTTGCCTGCAGGGAGCCCCCAGCAGCCCTTGTCCCAGCCTGGTTCTCCAGCCCCTGGTCCACCTGCC CAGATGGACCTGGAGCACCCACCTCAGCCTCCGTTTGCAACCCCCACATCTCTGCTGAAGAAGGAGCCCC CTGGTTATGAAGAGACTGTGACCCAGCAGCCTAAGCAGCAGGAAAATGGCTCCTCCAGTCAGCACATGGA TGATCTGTTTGATATTCTTATTCAGAGTGGAGAGATTTCAGCAGATTTCAAAGAGCCACCATCCCTACCA GGCAAGGAAAAGTCACCTCCAGCAGCAGCAGCGTATGGGCCTCCATTGACACCACAACCCTCGCCTTTGA GTGAACTCCCCCAAGCTGCTCCTCCACCAGGTTCCCCCACCCTCCCAGGGCGCCTTGAAGACTTCCTGGA GAGCAGCACAGGGCTGCCCCTGCTGACAAGTGGGCACGAGGGACCAGAACCCCTTTCCCTCATTGATGAC CTCCACAGCCAGATGCTGAGCAGCTCCGCCATCCTGGACCACCCCCCATCACCCATGGACACCTCTGAAT TGCACTTTGCTCCTGAGCCCAGCAGTGGTATGGGCCTGGACCTGGCTGTTGGCCACCTGGACAGCATGGA CTGGCTGGAGCTGTCGTCTGGTGGCCCTGTGCTCAGCCTGGCTCCCCTCAGCACTGCAGCCCCCAGCCTC TTCTCGATGGACTTCCTGGATGGACACGACTTGCAGCTCCACTGGGATTCCTGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211216 representing NM_001082536
Red=Cloning site Green=Tags(s) MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERAELVRMHILEETSAEPSLQAKQLKLKRARLADDL NEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSP LESRVSDPLPSATSISPTQVLSQLPMAPDPGETLFLAEQPPLPPAPLLPPSLANGSIVPTAKPAPTLIKQ SQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDKGAPAMDSSYAKILQQQQLFLQLQILNQQQQ QQQQQHYNYQAILPAPPKPSAETPGSSAPTPSRSLSTSSSPSSGTPGPSGLARQSSTALAAKPGALPANL DDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQVSPAPGAPKAPATTSVLSKAGEVVVAFPAALLST GSALVTAGLAPAEMVVATVTSNGMVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDAFGEMVTSPLTQL TLQASPLQIVKEEGARAASCCLSPGARAELEGLDKDQMLQEKDKQIEELTRMLQQKQQLVELLRLQLEQQ KRAQQPAPASSPVKRESGFSSCQLSCQPQGSAHAFGSGLVVPTTNHGDTQAPAPESPPVVVKQEAGPPEP DLAPSSQLLLGSQGTSFLKRVSPPTLVTDSTGTHLILTVTNKSADGPGLPAGSPQQPLSQPGSPAPGPPA QMDLEHPPQPPFATPTSLLKKEPPGYEETVTQQPKQQENGSSSQHMDDLFDILIQSGEISADFKEPPSLP GKEKSPPAAAAYGPPLTPQPSPLSELPQAAPPPGSPTLPGRLEDFLESSTGLPLLTSGHEGPEPLSLIDD LHSQMLSSSAILDHPPSPMDTSELHFAPEPSSGMGLDLAVGHLDSMDWLELSSGGPVLSLAPLSTAAPSL FSMDFLDGHDLQLHWDSCL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001082536 |
ORF Size | 2787 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001082536.1, NP_001076005.1 |
RefSeq Size | 4366 bp |
RefSeq ORF | 2790 bp |
Locus ID | 223701 |
UniProt ID | Q8K4J6 |
Gene Summary | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202660 | Mkl1 (untagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2, (10ug) |
CNY 6,808.00 |
|
MR211216 | Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CNY 6,808.00 |
|
MR211216L3 | Lenti ORF clone of Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CNY 9,030.00 |
|
MR211216L4 | Lenti ORF clone of Mkl1 (mGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CNY 9,030.00 |