Smcr8 (BC085095) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2310076G09Rik; AI642055; D030073L15Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211250 representing BC085095 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGCGCCCCTGATGCGGTGGCCTTCACCAAGGAAGATGAATACGAGGAAGAACCTTACAATGAGC CCGCTTTGCCTGAGGAGTACTCAGTCCCTCTCTTTCCTTATGCCAGCCAGGGGGCAAACCCCTGGTCTAA ACTGTCTGGGGCCAAGTTCTCCAGGGACTTCATCCTCATTTCCGAGTTCTCTGAGCAGGTGGGACCCCAG CCCTTGCTTACCATCCCCAATGACACCAAAGTTTTTGGCACTTTTGATCTTAATTACTTCTCTTTGCGGA TTATGTCGGTGGATTACCAGGCTTCATTCGTAGGCCATCCTCCCGGTTCTGCCTACCCCAAGTTGAACTT TGTGGAAGACTCTAAAGTGGTACTGGGAGACTCTAAGGAAGGGGCCTTTGCATATGTGCACCACCTCACC TTGTACGACCTGGAGGCCAGGGGCTTTGTGAGGCCCTTTTGTATGGCTTATATCTCCGCAGACCAGCATA AAATCATGCAGCAGTTCCAAGAGCTCTCGGCCGAATTTTCCAAAGCATCTGAGTGCTTGAAGATGGGCAA CAGGAAGGCATTCGCTGGGGAACTTGAAAAAAAGCTGAAAGACTTGGATTACACAAGGACAGTGCTACAC ACAGAAACCGAGATCCAGAAGAAAGCCAACGACAAGGGTTTTTATTCTTCTCAGGCGATTGAGAAAGCCA ATGAACTGGCCAATGTGGAGAAGTCCATCATCGAACATCAAGATTTGCTGAGGCAGATCCGCTCATACCC TCGTCAGAAGACGAAGATCCCTGACTTGCAGCCTGGTGATACAGAGCATACCCAGGATCAGGCTGACCAG GTATCCACTACCTCTAATCCTGAGGAGTCCGCTAATGCAGACCTTTATACCTGCAGACCGGCTTACACCC CCAAACTCATCAAAGCAAAGTCCACCAAGTGTTTTGACAAGAAGTTGAAGACCTTGGAGGAACTCTGTGA CACTGAGTATTTCACTCAGACCCTGGCCCAGCTTAGCCACATTGAACACATGTTCAGAGGAGACCTGTGC TACCTCCTGACCAGTCAGATTGACAGAGTGCTTCGAAAACAACAGCCCATAACGAATTTCCTCTTTGAAG ATTTTGTAGAGGTGGATGACAGGATGGAAAAGCAAGAGAATGTACCCTCTCAGCCCAGTCAGGACAGGCT GCCTCCCAAGCCTGTAGAAGAATGCCCCATTCCTAAAGTGTTAATTAGCGTTGGCTCTTACAAGTCCAGT GTGGAGTCTGTATTGATCAAGATGGAGCAGGAACTTGGCGATGAAGAGTACACGGGGGTGGAGGCAACAG AGGCACGCAGTTTTGACCCCCAAGAGAACCTGGACTACCTGGATATGGATATGAAAGGGAGCATCAGTAG TGGGGAAAGCATTGAGGTGCTGGGCACCGAGAAGTCAGCCTCTGTGTTGTCGAAATCTGACAGCCAGGCC AGCCTCACCGTGCCATTGAGCCCCCATGTAGTCCGTAGCAAAGCGGTCAGCCACAGGACAATCAGCGAGG ACAGCATTGAAGTCTTAAGTACCTGCCCTTCTGAGGCCCTCATTCCTGATGACTTTAAGGCCAGTTACCC AAGTGCCATTAATGAAGAAGAAGCCTATGCGGATAATGAGGGGGCCATCCATTTCCAGGCAAGTGCCGGC TCACCAGAACCGGATGAGACTCAGGAGGGCAACTTGGAAAATATCCCATCCCAAATAGACTCCAGCTGCT GTATTGGAAAGGAGAGTGAAGGTCACTTGGTGCCTCTCCCAACCCCAGCCTACACTCTTTCTGATGAGGA CAGTGTGGTGAGCATTCCCCCACAGCGCTACATACAGAAGGACCAGGGGCTCCACGTGGACTTCGGAGTG GAAAACACTGACCCTTCTCCCCGAGACAACAGTTGTGAAATGTTCCCAGCTTATGAGCTGGATCCAAGCT GCCTTCTGGCTAGCCGAGATGTTAGTAAGATGAGCCTGGATAACTACTCGGATACCACTAGCTACATGGG CAGTGCGGCCTCTACCAGCTCAGACAGAATCCCCTCAGCACCTCCTGCTGGCCTATCCTCTGAGAGGCAC AAAAAGAGAGCTGGCCAGAATGCCTTAAAATTCATCCGCCAGTACCCTTTTGCCCACCCAGCCATCTACT CCCTGCTCAGTGGGAGGACACTTGTGGTCCTGGGCGAAGATGAAACCATTGTCAGGAAGCTGGTGACTGC ACTGTCCATCTTTGTTCCCAACTATGGCTGCTATGCCAAGCCGGTGAAGCACTGGATTTCTTCCCCTTTG CATATTATGGATTTCCAGAAGTGGAAGCTTATTGGCCTACAAAGAGTGGCCTCTCCTGCCAATGTGGGTA CCCTCCATACCCTGAGCCGTTACAGCCGTTATACAAGCATCCTGGACCTGGACAGCAAGACCCTACGCTG TCCCCTATACAGGGGTACACTGGTACCCCGCCTGGCTGACCATCGCACTCAGATCAAGCGGGGCAGCACA TACTACCTTCACGTCCAGAGCATGCTCACCCAGCTCTGCTCCAAGGCATTCCTCTACACCTTTTGTCACC ACTTGCATCTGCCCGCCCACAGTGAGGAGACACAAGAAGCAGTGGCCAGCAGACAAACCAGCTTCCTAAA GCTAAACCTGGGGCTCGTGAACGAGGACATCAGAGTGGTACAGTACCTGGCTGAGCTGTTGAAACTGCAC TATATGCAGGAGTCACCGGGGACCACCCACCCACTGCTCAGGTTTGACTACGTCCCCAGCTTTTTGTACA AAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211250 representing BC085095 Red=Cloning site Green=Tags(s) MISAPDAVAFTKEDEYEEEPYNEPALPEEYSVPLFPYASQGANPWSKLSGAKFSRDFILISEFSEQVGPQ PLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLT LYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSKASECLKMGNRKAFAGELEKKLKDLDYTRTVLH TETEIQKKANDKGFYSSQAIEKANELANVEKSIIEHQDLLRQIRSYPRQKTKIPDLQPGDTEHTQDQADQ VSTTSNPEESANADLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLC YLLTSQIDRVLRKQQPITNFLFEDFVEVDDRMEKQENVPSQPSQDRLPPKPVEECPIPKVLISVGSYKSS VESVLIKMEQELGDEEYTGVEATEARSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSASVLSKSDSQA SLTVPLSPHVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEEAYADNEGAIHFQASAG SPEPDETQEGNLENIPSQIDSSCCIGKESEGHLVPLPTPAYTLSDEDSVVSIPPQRYIQKDQGLHVDFGV ENTDPSPRDNSCEMFPAYELDPSCLLASRDVSKMSLDNYSDTTSYMGSAASTSSDRIPSAPPAGLSSERH KKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDETIVRKLVTALSIFVPNYGCYAKPVKHWISSPL HIMDFQKWKLIGLQRVASPANVGTLHTLSRYSRYTSILDLDSKTLRCPLYRGTLVPRLADHRTQIKRGST YYLHVQSMLTQLCSKAFLYTFCHHLHLPAHSEETQEAVASRQTSFLKLNLGLVNEDIRVVQYLAELLKLH YMQESPGTTHPLLRFDYVPSFLYKI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC085095
ORF Size	2807 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC085095, AAH85095
RefSeq Size	3049 bp
RefSeq ORF	2807 bp
Locus ID	237782
Gene Summary	Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27617292). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (By similarity). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity (PubMed:27617292). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (By similarity). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (By similarity).[UniProtKB/Swiss-Prot Function]

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