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Slc8a1 (BC079673) Mouse Tagged ORF Clone
CAT＃: MG211305
- TrueORF®
Slc8a1 (tGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156)
ORF Plasmid: DDK
"BC079673" in other vectors (4)
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CNY 8,616.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI852629; AV344025; D930008O12Rik; Ncx1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211305 representing BC079673
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGATTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAGCTCTGGTGGCTCTCTTGT TTTCCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC TGGCTCATATTACTGTAAGAAAGGGGTGATCTTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAC AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTTGGAGTTTCTATTATTGCAG ACCGGTTTATGTCCTCTATAGAGGTCATCACCTCTCAAGAGAAAGAAATAACGATAAAGAAACCGAATGG AGAGACCACCAAGACGACGGTGAGAATCTGGAACGAGACTGTGTCGAACCTGACCTTGATGGCCCTGGGA TCTTCTGCTCCTGAGATTCTCCTGTCAGTCATTGAAGTGTGCGGCCATAACTTCACCGCAGGGGACCTGG GTCCCAGCACCATCGTGGGAAGTGCTGCCTTTAACATGTTCATCATAATCGCACTCTGTGTTTACGTGGT CCCTGATGGAGAGACAAGGAAGATCAAGCATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT GCCTATACCTGGCTTTATATAATCTTGTCTGTCAGCTCTCCTGGAGTTGTGGAGGTCTGGGAAGGCTTGC TTACTTTCTTCTTCTTTCCCATCTGCGTTGTGTTCGCGTGGGTAGCAGACAGGCGGCTTCTCTTTTACAA GTATGTCTACAAGCGGTACAGGGCCGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA GCTTCCAAAACTGAAATCGAAATGGATGGGAAAGTGGTCAACTCTCATGTTGACAATTTCTTAGATGGGG CTCTGGTTTTGGAAGTTGATGAGAGGGACCAAGATGATGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT GAAGGAACTTAAGCAGAAGCATCCTGAGAAAGAAATTGAGCAATTAATAGAATTAGCCAACTACCAGGTC CTAAGTCAACAGCAGAAAAGCCGAGCATTTTACAGGATTCAAGCTACTCGCCTGATGACCGGAGCTGGCA ACATCTTGAAGAGGCACGCAGCTGATCAAGCAAGGAAGGCTGTCAGTATGCATGAAGTCAACATGGAAAT GGCTGAAAACGACCCAGTCAGTAAGATCTTCTTTGAGCAAGGAACATACCAGTGTCTAGAGAACTGTGGT ACTGTGGCCCTCACCATTATGCGCAGAGGGGGCGACTTGAGCACCACTGTGTTTGTTGACTTCAGGACAG AAGACGGCACAGCCAATGCTGGGTCTGATTATGAATTCACGGAAGGGACTGTGATCTTCAAACCAGGGGA GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTCCTTGTG CATCTTAGCAATGTCAGAGTCTCTTCAGATGTTTCAGAAGATGGCATACTAGAATCCAATCACGCTTCTT CAATTGCTTGTCTTGGGTCACCCAGCACTGCCACCATAACCATTTTTGATGATGACCATGCAGGCATCTT TACATTTGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTTTTGAGAACC TCTGGAGCTCGAGGAAATGTTATCATTCCCTACAAAACTATTGAAGGCACAGCCCGAGGTGGAGGGGAAG ACTTTGAGGACACCTGTGGAGAGCTCGAATTCCAGAACGATGAAATAGTCAAAACAATATCAGTCAAGGT AATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCTTCATTGAGATTGGAGAGCCCCGTCTGGTGGAG ATGAGTGAGAAGAAAGGTGGCTTCACATTAACAGGCCAACCTATCTTCAGGAAGGTCCATGCTAGAGATC ATCCGATTCCCTCTACTGTAATCACCATCTCAGAGGAATATGATGACAAGCAGCCACTGACCAGCAAAGA AGAGGAGGAGAGGCGCATTGCGGAAATGGGGCGCCCCATCCTAGGCGAGCACACCAAGCTGGAGGTGATC ATCGAAGAGTCTTACGAATTCAAGAGCACTGTGGACAAACTCATTAAGAAGACGAACCTGGCCCTTGTGG TGGGGACCAACAGCTGGAGAGAGCAGTTTATCGAAGCCATCACTGTCAGCGCTGGGGAAGATGACGATGA TGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGTTTTGATTACGTGATGCACTTTCTCACAGTGTTCTGG AAGGTTCTGTTTGCCTTCGTCCCACCTACAGAATACTGGAATGGCTGGGCCTGCTTCATTGTCTCCATCC TCATGATCGGCCTACTGACCGCCTTCATTGGAGACCTGGCTTCCCACTTTGGCTGCACCATTGGTCTGAA AGATTCCGTGACTGCCGTTGTGTTTGTTGCTCTTGGAACCTCGGTGCCAGACACATTTGCCAGCAAAGTA GCAGCTACCCAGGACCAGTATGCAGATGCGTCTATAGGCAATGTCACTGGAAGCAATGCTGTGAATGTCT TCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGCCATCTACCATGCGGCCAACGGGGAACAGTTCAAAGT GTCCCCCGGCACGCTAGCTTTCTCTGTCACTCTCTTCACTATTTTTGCTTTCATCAACGTGGGGGTGCTG CTGTATCGGCGGAGGCCAGAAATAGGAGGTGAGCTGGGAGGGCCCCGGACTGCCAAGCTCCTCACATCTT CCCTGTTTGTGCTCCTGTGGCTCTTGTACATTTTCTTCTCCTCCCTGGAAGCCTACTGCCACATAAAGGG CTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211305 representing BC079673
Red=Cloning site Green=Tags(s) MLRLSLPPNVSMGFRLVALVALLFSHVDHITADTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGD KIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALG SSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIF AYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRP ASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPEKEIEQLIELANYQV LSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMEMAENDPVSKIFFEQGTYQCLENCG TVALTIMRRGGDLSTTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLV HLSNVRVSSDVSEDGILESNHASSIACLGSPSTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRT SGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVE MSEKKGGFTLTGQPIFRKVHARDHPIPSTVITISEEYDDKQPLTSKEEEERRIAEMGRPILGEHTKLEVI IEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCFDYVMHFLTVFW KVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKV AATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTLFTIFAFINVGVL LYRRRPEIGGELGGPRTAKLLTSSLFVLLWLLYIFFSSLEAYCHIKGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC079673 |
| ORF Size | 2876 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079673, AAH79673 |
| RefSeq Size | 4032 bp |
| RefSeq ORF | 2876 bp |
| Locus ID | 20541 |
| Gene Summary | Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:8659820). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:10967099). Required for normal embryonic heart development and the onset of heart contractions (PubMed:10967099).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220757 | Slc8a1 (untagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156), (10ug) |
CNY 9,120.00 |
|
| MR211305 | Slc8a1 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156) |
CNY 7,016.00 |
|
| MR211305L3 | Lenti ORF clone of Slc8a1 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156) |
CNY 7,320.00 |
|
| MR211305L4 | Lenti ORF clone of Slc8a1 (mGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156) |
CNY 7,320.00 |
