Slc8a1 (BC079673) Mouse Tagged ORF Clone

CAT#: MG211305

  • TrueORF®

Slc8a1 (tGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (cDNA clone MGC:90693 IMAGE:30532156)

ORF Plasmid: DDK tGFP



  "BC079673" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 8,616.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI852629; AV344025; D930008O12Rik; Ncx1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211305 representing BC079673
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCGATTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAGCTCTGGTGGCTCTCTTGT
TTTCCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC
TGGCTCATATTACTGTAAGAAAGGGGTGATCTTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAC
AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTTGGAGTTTCTATTATTGCAG
ACCGGTTTATGTCCTCTATAGAGGTCATCACCTCTCAAGAGAAAGAAATAACGATAAAGAAACCGAATGG
AGAGACCACCAAGACGACGGTGAGAATCTGGAACGAGACTGTGTCGAACCTGACCTTGATGGCCCTGGGA
TCTTCTGCTCCTGAGATTCTCCTGTCAGTCATTGAAGTGTGCGGCCATAACTTCACCGCAGGGGACCTGG
GTCCCAGCACCATCGTGGGAAGTGCTGCCTTTAACATGTTCATCATAATCGCACTCTGTGTTTACGTGGT
CCCTGATGGAGAGACAAGGAAGATCAAGCATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT
GCCTATACCTGGCTTTATATAATCTTGTCTGTCAGCTCTCCTGGAGTTGTGGAGGTCTGGGAAGGCTTGC
TTACTTTCTTCTTCTTTCCCATCTGCGTTGTGTTCGCGTGGGTAGCAGACAGGCGGCTTCTCTTTTACAA
GTATGTCTACAAGCGGTACAGGGCCGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA
GCTTCCAAAACTGAAATCGAAATGGATGGGAAAGTGGTCAACTCTCATGTTGACAATTTCTTAGATGGGG
CTCTGGTTTTGGAAGTTGATGAGAGGGACCAAGATGATGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT
GAAGGAACTTAAGCAGAAGCATCCTGAGAAAGAAATTGAGCAATTAATAGAATTAGCCAACTACCAGGTC
CTAAGTCAACAGCAGAAAAGCCGAGCATTTTACAGGATTCAAGCTACTCGCCTGATGACCGGAGCTGGCA
ACATCTTGAAGAGGCACGCAGCTGATCAAGCAAGGAAGGCTGTCAGTATGCATGAAGTCAACATGGAAAT
GGCTGAAAACGACCCAGTCAGTAAGATCTTCTTTGAGCAAGGAACATACCAGTGTCTAGAGAACTGTGGT
ACTGTGGCCCTCACCATTATGCGCAGAGGGGGCGACTTGAGCACCACTGTGTTTGTTGACTTCAGGACAG
AAGACGGCACAGCCAATGCTGGGTCTGATTATGAATTCACGGAAGGGACTGTGATCTTCAAACCAGGGGA
GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTCCTTGTG
CATCTTAGCAATGTCAGAGTCTCTTCAGATGTTTCAGAAGATGGCATACTAGAATCCAATCACGCTTCTT
CAATTGCTTGTCTTGGGTCACCCAGCACTGCCACCATAACCATTTTTGATGATGACCATGCAGGCATCTT
TACATTTGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTTTTGAGAACC
TCTGGAGCTCGAGGAAATGTTATCATTCCCTACAAAACTATTGAAGGCACAGCCCGAGGTGGAGGGGAAG
ACTTTGAGGACACCTGTGGAGAGCTCGAATTCCAGAACGATGAAATAGTCAAAACAATATCAGTCAAGGT
AATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCTTCATTGAGATTGGAGAGCCCCGTCTGGTGGAG
ATGAGTGAGAAGAAAGGTGGCTTCACATTAACAGGCCAACCTATCTTCAGGAAGGTCCATGCTAGAGATC
ATCCGATTCCCTCTACTGTAATCACCATCTCAGAGGAATATGATGACAAGCAGCCACTGACCAGCAAAGA
AGAGGAGGAGAGGCGCATTGCGGAAATGGGGCGCCCCATCCTAGGCGAGCACACCAAGCTGGAGGTGATC
ATCGAAGAGTCTTACGAATTCAAGAGCACTGTGGACAAACTCATTAAGAAGACGAACCTGGCCCTTGTGG
TGGGGACCAACAGCTGGAGAGAGCAGTTTATCGAAGCCATCACTGTCAGCGCTGGGGAAGATGACGATGA
TGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGTTTTGATTACGTGATGCACTTTCTCACAGTGTTCTGG
AAGGTTCTGTTTGCCTTCGTCCCACCTACAGAATACTGGAATGGCTGGGCCTGCTTCATTGTCTCCATCC
TCATGATCGGCCTACTGACCGCCTTCATTGGAGACCTGGCTTCCCACTTTGGCTGCACCATTGGTCTGAA
AGATTCCGTGACTGCCGTTGTGTTTGTTGCTCTTGGAACCTCGGTGCCAGACACATTTGCCAGCAAAGTA
GCAGCTACCCAGGACCAGTATGCAGATGCGTCTATAGGCAATGTCACTGGAAGCAATGCTGTGAATGTCT
TCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGCCATCTACCATGCGGCCAACGGGGAACAGTTCAAAGT
GTCCCCCGGCACGCTAGCTTTCTCTGTCACTCTCTTCACTATTTTTGCTTTCATCAACGTGGGGGTGCTG
CTGTATCGGCGGAGGCCAGAAATAGGAGGTGAGCTGGGAGGGCCCCGGACTGCCAAGCTCCTCACATCTT
CCCTGTTTGTGCTCCTGTGGCTCTTGTACATTTTCTTCTCCTCCCTGGAAGCCTACTGCCACATAAAGGG
CTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211305 representing BC079673
Red=Cloning site Green=Tags(s)

MLRLSLPPNVSMGFRLVALVALLFSHVDHITADTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGD
KIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALG
SSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIF
AYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRP
ASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPEKEIEQLIELANYQV
LSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMEMAENDPVSKIFFEQGTYQCLENCG
TVALTIMRRGGDLSTTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLV
HLSNVRVSSDVSEDGILESNHASSIACLGSPSTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRT
SGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVE
MSEKKGGFTLTGQPIFRKVHARDHPIPSTVITISEEYDDKQPLTSKEEEERRIAEMGRPILGEHTKLEVI
IEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCFDYVMHFLTVFW
KVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKV
AATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTLFTIFAFINVGVL
LYRRRPEIGGELGGPRTAKLLTSSLFVLLWLLYIFFSSLEAYCHIKGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079673
ORF Size 2876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079673, AAH79673
RefSeq Size 4032 bp
RefSeq ORF 2876 bp
Locus ID 20541
Gene Summary Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:8659820). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:10967099). Required for normal embryonic heart development and the onset of heart contractions (PubMed:10967099).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...