Pum2 (BC041773) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Pumm2, mKIAA0235
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211386 representing BC041773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA GACTGCATGGGGAACTTCTCATCATTCAATGTCCCAGCCTATTATGGTGCAGAGAAGATCTGGACAGAGT TTTCATGGAAACAGTGAAGTAAATGCAATTCTTTCTCCACGCTCAGAAAGTGGAGGCCTTGGTGTGAGCA TGGTAGAATATGTATTAAGTTCCTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAACTTTTGG TACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG GACCAAAACAGAGATCTTAAACAAGATGATGAGGACTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAA TGGATGCCGATTGCAAAGATTTTAATCGCACTCCTGGAAGTCGCCAAGCCTCTCCAACTGAAGTAGTTGA GCGCCTTGGCCCTAGTACTAATCCCCCAGAAGGATTGGGCCCTCTTCCTAATCCGACAGCGAATAAACCA CTTGTTGAAGAATTTTCAAACCCTGAAACTCAGAATCTGGATGCAATGGACCAAGTTGGTCTGGATTCCT TACAGTTTGACTATCCTGGTAATCAGGTACCCATGGATTCTTCAGGAGCTACTGTAGGCCTTTTTGACTA CAATTCCCAACAGCAGCTCTTTCAGAGGACTAGTGCACTAACAGTTCAGCAGTTAACTGCAGCTCAGCAG CAGCAGTATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAG CTGCATTTGTGCCAAATCCATATATTATTAGTGCTGCTCCTCCAGGGACTGACCCGTATACTGCAGCAGG ATTGGCTGCAGCAGCAACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGTGTTCCATGGGGA GTGTATCCAGCCAATTTATTTCAGCAACAAGCTGCAGCTGCGGCAAGCAACACAGCCAACCAGCAAGCAG CATCACAAGCTCAGCCTGGACAGCAGCAGGTTCTTCGTCCTGGAGCAGGTCAGCGTCCTATTACTCCAAG TCAGGGCCAACAAGGGCAGCAAGCAGAGTCACTTGCAGCAGCTGCAAACCCAACTTTGGCTTTTGGTCAG AGTCTTGCTGCAGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCT TAGTGGTTGGCCCCGGAGCAAGAACTGGCCTTGGAGCTCCAGTACGATTAATGGCTCCAACACCTGTCTT AATAAGTTCAACAGCAGCACAAGCTGCAGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAATAGTCTTACA GGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAGCAGCAGCAGCAAC CAAGCACTAACCTGCAGTCTAATTCATTTTATGGGAGCAGCTCTTTGACTAACAGCTCCCAGAGCAGCTC TTTATTCTCTCATGGACCTGGCCAACCTGGAAGTGCGTCTCTTGGCTTTGGAAGTGGTAGCTCTTTAGGA GCTGCTATAGGCTCAGCTCTCAGTGGATTTGGCTCATCAGGAGGACTGACAAACGGTAGTGGTCGGTATA TCTCTGCAGCACCTGGAGCAGAAGCAAAGTACCGAAGTGCTTCAAGCACTTCCAGTCTATTTAGCTCCAG CAGCCAGCTCTTTCCTCCTTCTCGGCTCCGCTATAATAGATCTGACATCATGCCCTCCGGCCGCAGTAGG TTATTGGAAGATTTCAGGAACAACCGCTTCCCCAACCTTCAGCTCAGAGACTTAATTGGACATATAGTCG AGTTTTCTCAAGACCAGCATGGTTCCAGATTCATACAGCAAAAGCTAGAGAGAGCTACTCCAGCTGAGCG ACAAATAGTATTTAATGAAATTCTACAGGCAGCCTATCAATTAATGACAGATGTTTTTGGAAACTATGTT ATACAGAAGTTTTTTGAGTTTGGAAGTTTGGATCAGAAATTAGCCCTGGCTACTCGTATTCGTGGTCATG TTCTACCATTAGCCTTGCAGATGTATGGCTGCCGTGTTATTCAAAAGGCGTTAGAATCTATTTCTTCTGA TCAGCAGAGTGAAATGGTTAAGGAACTAGATGGCCATGTACTTAAATGTGTGAAAGATCAAAATGGAAAC CATGTTGTACAGAAATGCATTGAATGTGTTCAGCCACAGTCACTGCAGTTCATCATCGATGCTTTCAAAG GACAAGTATTTGTGCTTTCAACCCATCCTTATGGCTGCAGAGTCATTCAGCGTATCTTAGAGCACTGCAC GGCAGAGCAGACCTTACCCATCTTAGAAGAACTTCACCAACACACAGAACAGTTGGTACAGGATCAGTAT GGCAATTATGTTATTCAGCATGTACTGGAACATGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAA TCAGAGGAAAGGTCTTAGCCCTGAGTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCA TGCCTCCCGTGCTGAGAGAGCTTTACTGATTGATGAGGTCTGCTGTCAGAATGATGGTCCTCACAGTGCC TTATACACCATGATGAAGGATCAGTATGCCAACTATGTGGTTCAGAAGATGATTGATATGGCTGAGCCTG CGCAGAGAAAGATAATCATGCACAAGATTCGACCTCATATTACTACTCTTCGCAAATACACATATGGGAA GCATATACTGGCCAAGTTGGAAAAATACTATCTGAAAAACAGCCCAGATCTAGGGCCAATTGGAGGACCA CCAAATGGGATGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211386 representing BC041773 Red=Cloning site Green=Tags(s) MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGTSHHSMSQPIMVQRRSGQS FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGTFGTRDAETDGPEKGDQKGKASPFEE DQNRDLKQDDEDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPSTNPPEGLGPLPNPTANKP LVEEFSNPETQNLDAMDQVGLDSLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTSALTVQQLTAAQQ QQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPWG VYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQRPITPSQGQQGQQAESLAAAANPTLAFGQ SLAAGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSTAAQAAAAAAAAGGTANSLT GSTNGLFRPIGTQPPQQQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSASLGFGSGSSLG AAIGSALSGFGSSGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRSDIMPSGRSR LLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQIVFNEILQAAYQLMTDVFGNYV IQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGN HVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQY GNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSA LYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGP PNGML TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC041773
ORF Size	2957 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC041773, AAH41773
RefSeq Size	3295 bp
RefSeq ORF	2957 bp
Locus ID	80913
Gene Summary	Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]

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