Card10 (BC060203) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Bimp1, CARMA3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211464 representing BC060203 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGCCGGGCGGACGCGGGCGAGGCTGACGAGGAGGCTGGGGCGGGTTCGGGGTCGGAGGCCGAAG AGGACGCGCTGTGGGAGCGGATCGAGGGCGTCCGGCACCGACTGACGCGCGCCCTGAATCCGGCCAAGCT CACGCCGTATCTGCGCCAGTGCCGCGTCCTGGACGAACAGGACGAGGAGGAGGTGCTCAGCACCTACCGT TTCCCGTGCCGCGCTAACCGTACCGGGCGCCTCATAGACATTTTACGCTGCCGGGGCAAGAGGGGCTTCG AGGCCTTCCTGGAAGCCCTGGAATTCTACTACCCAGAACACTTCACGCTGCTCACTGGCCAGGAACCTGC CCAACGCTGCTCCATGATCCTTGATGAGGAGGGTCCCGAGGGCCTGACTCAGTTCTTGATGACTGAGGTC CGGAGGCTTCGGGAAGCTCGGAAGAGCCAATTGCACCGAGAGCAGCAACTGCAGGCCCGGGGCCGGGCCC TGGAGGAGGAGCGGGCAGGGCTAGAGCAGAGGCTTCGGGAGCAGCAGCAGGCACAGGAGCGCTGCCAGCG ACTGCGGGAGGACTGGGAGGCTGGCAGCCTGGAGCTGCTGCGCCTCAAGGATGAGAACTACATGATCGCC ATGCGCCTGGCTCAGCTCAGCGAGGAGAAGAATTCGGCTGTGCTGCGCAGCCGAGACCTGCAGCTGGCGG TGGACCAGCTCAAGCTCAAGGTCAGCCGGCTGGAGGAAGAGTGTGCGCTGCTGAGGAGGGCCAGGGGCCC ACCGCCTGGGGCTGAGGAGAAGGAGAGGGAGCCAGATGGCGCTGATCTTCTCTCTGAGCTTCGTGCAGAG AACCAGCGGCTGACGGCTTCACTGCAGGAGCTTCAGGAAGGTCTTCAGCAGGAGATGAGCCGGCCAGGAG CTGCAGGCTCTGAACGTATCCTACTGGACATACTGGAGCACGACTGGAGGGAGGCGCAGGACAGCAGGCA GGAGCTGTGTCAGAAGCTTCATGCTGTGCAGGGCGAGCTACAGTGGGCAGAGGAGCTGCGGGACAAGTAC CTGCAAGAGATGGAGGATCTGCGGCTCAAACATCGAACGCTGCTGAAGGACTGTGACCTCTATAAGCACC GCATGGCCACTGTGTTGGCCCAGCTGGAGGAGATTGAGAAAGAGCGGGACCAGGCAATCCAGAGCCGAGA CCGGATCCAGCTGCAGTATTCTCAGAGCCTCATTGAGAAGGACCAGTACCGCAAGCAGGTGCGGGGGCTG GAGGCGGAGCGCGATGAGCTGCTCACCACAGTCACCAGCCTGGAGGGGACCAAGGCCATGCTGGAGGCAC AGCTGCAGCGGACACAGGGGGGTTCCTGTCTCAAGGCCTGTGCCTCCTCCCACTCCCTGTGCTCCAACCT CAGCAGCACCTGGAGCCTCAGCGAGTTCCCATCGCCCCTGGGAGGCCCAGAAGCTACAGGAGAAGCCGGG GGATCTGAGCCCCACACCTCGGAGGAAGCCACAGACAGCGAGAAGGAGATCAACCGGCTCTCTATCCTGC CTTTCCCACCCAGTGCTGGTTCCATACTCCGCCGGCAGCGTGAGGAAGACCCGGAGCCCCCTAAGAGGTC TTTTAGCAGCATGTCGGATATCACAGGGAGTGTGACCCTTAAGCCATGGTCCCCTGGCCTCTCTTCATCA TCCTCGTCTGACAGTGTGTGGCCTTTGGGAAAGCCGGAAGGCCTCCTGGCTCGGGGCTGTGGCCTGGACT TCCTCAATAGGTCTCTGGCCATCCGAGTGTCCGGCTGGAGTCCCCCCGCAGGCCTGGATCCCCAGGATAA GAGTCCAGATAGCATGCCGGGCCTTGGAGACAGGTGGTCTGGGGCGGTGGTGCGAAGGGTGTTGTCTGGG CCTGGGTCAGCCAGGACAGAACAGAAAGAGCCGAGGGCGGAAGGCACTGGTCTGGAGGGGGCAGGCCTGG AAGCGGAGGCCCAGCAGAGGACCTTGCCCTGGAACCAGAGCTCCACCCTCCCCTTCCTGCTGGACTCAAA GGCCTGTCATTCCTTCCATGAGGCCCTGGATGCCTGGGCAAAGGGACCGGGTGCCGAGCCCTTCTACATT CGAGCCAACTTCTCCCTGCCCGAGCGGTCGGATCCCCATGCCCTTTGTGTGAAAGCCCAAGAGATCCTGC GGTTGGTGGACCCAGCCCACAAGCGGAGGCAGGAGTGGTTCTGTACTCGAGTGGACACCCTCACCTTACG GGACCTGGACCGGGGCACCGTGCCCAATTATCAGAGAGCTCAGCAGCTCCTAGAAGTCCAGGAAAAATAC TTGATCTCCAGCAGACACCGAAGTCCTCGAAGCAACCTGAAGAAGCGAGCCCTAGGGTTGGTGAGGCCCA AGCCTGCAGGGGGCACTGCAGGAGACTCAGCAGAACAGCTCCCTGCAGAGCCCTGCTCAGAGCTAGAACG AAGCCTAAAACCCTACAGCCTGGTGCGACCGCTGTTGGTGTCTGCCTTGAGGCCTGTGGTCCTCCTGCCT GAGTGCCTGGCGCCTCGCCTCATCCGTAACCTGCTGGATCTGCCAAGCTCCAGGCTGGATTTCCAAGTGT GCCCAGCAGAGAGCCTCTCTGGGGAGGAACAGTGTACGTCCTCAGCTCCCGGAGCCCCCAAAGCCTGGCC GGCCACTGCTGGGCTAGGCAGCAGGATCCGTGCCATCCAGGAGTCTGTCGGGAAGAAGCACTGCCTGTTG GAGCTGGGTGCACGGGGAGTGCGGGAGTTGGTGCACAGCGAGGTCTACCCCATTGTCATCCACGTGGAGG TGACTGAGAAGAATGTCCGTGAGATCAGGGGCCTGTTGGGCCGGCCGGGCTGGCGGGATTCAGAACTGCT CCGTCAGTGTCGGGGCTCAGAACAGTGGCTCTGGGGACTGCCGTGTTCCTGGGTGCAGGTGCCTGCGCAT GCGTGGGGCCATGCAGAGGAGCTGGCCAAGGTGGTGCGTGGCCGAATCTTGCAGGAACAGGCTCGCCTTG TGTGGGTGGAGCGAGGCAGCAGCAGAGGGGGCAGTGGCAGCAGTAGTGAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211464 representing BC060203 Red=Cloning site Green=Tags(s) MQGRADAGEADEEAGAGSGSEAEEDALWERIEGVRHRLTRALNPAKLTPYLRQCRVLDEQDEEEVLSTYR FPCRANRTGRLIDILRCRGKRGFEAFLEALEFYYPEHFTLLTGQEPAQRCSMILDEEGPEGLTQFLMTEV RRLREARKSQLHREQQLQARGRALEEERAGLEQRLREQQQAQERCQRLREDWEAGSLELLRLKDENYMIA MRLAQLSEEKNSAVLRSRDLQLAVDQLKLKVSRLEEECALLRRARGPPPGAEEKEREPDGADLLSELRAE NQRLTASLQELQEGLQQEMSRPGAAGSERILLDILEHDWREAQDSRQELCQKLHAVQGELQWAEELRDKY LQEMEDLRLKHRTLLKDCDLYKHRMATVLAQLEEIEKERDQAIQSRDRIQLQYSQSLIEKDQYRKQVRGL EAERDELLTTVTSLEGTKAMLEAQLQRTQGGSCLKACASSHSLCSNLSSTWSLSEFPSPLGGPEATGEAG GSEPHTSEEATDSEKEINRLSILPFPPSAGSILRRQREEDPEPPKRSFSSMSDITGSVTLKPWSPGLSSS SSSDSVWPLGKPEGLLARGCGLDFLNRSLAIRVSGWSPPAGLDPQDKSPDSMPGLGDRWSGAVVRRVLSG PGSARTEQKEPRAEGTGLEGAGLEAEAQQRTLPWNQSSTLPFLLDSKACHSFHEALDAWAKGPGAEPFYI RANFSLPERSDPHALCVKAQEILRLVDPAHKRRQEWFCTRVDTLTLRDLDRGTVPNYQRAQQLLEVQEKY LISSRHRSPRSNLKKRALGLVRPKPAGGTAGDSAEQLPAEPCSELERSLKPYSLVRPLLVSALRPVVLLP ECLAPRLIRNLLDLPSSRLDFQVCPAESLSGEEQCTSSAPGAPKAWPATAGLGSRIRAIQESVGKKHCLL ELGARGVRELVHSEVYPIVIHVEVTEKNVREIRGLLGRPGWRDSELLRQCRGSEQWLWGLPCSWVQVPAH AWGHAEELAKVVRGRILQEQARLVWVERGSSRGGSGSSSEA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060203
ORF Size	3065 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC060203, AAH60203
RefSeq Size	4748 bp
RefSeq ORF	3065 bp
Locus ID	105844
Gene Summary	Activates NF-kappa-B via BCL10 and IKK.[UniProtKB/Swiss-Prot Function]

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