Magi2 (BC059005) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Acvri1, Acvrip1, mKIAA0705, S-SCAM, Magi-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211649 representing BC059005 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATTGGAGAAAAGTGGTGCTCTCCTAGAAAGCGGGACCTATGAAGACAACTACTACGGTACCCCGA AGCCTCCAGCTGAACCAGCACCATTATTAAATGTAACAGACCAGATACTTCCGGGAGCTACTCCAAGTGC TGAGGGGAAGCGGAAAAGAAATAAGTCAGTGACCAACATGGAGAAAGCAAGTATAGAGCCTCCAGAGGAG GAAGAAGAAGAAAGGCCTGTAGTCAATGGAAACGGCGTGGTCATAACCCCAGAATCCAGTGAACATGAAG ACAAAAGTGCAGGTGCCTCAGGGGAGACACCCTCCCAGCCTTACCCTGCACCCGTGTACAGCCAGCCCGA AGAGCTCAAGGACCAGATGGACGATACAAAGCCAACAAAGCCTGAGGAGAACGAGGACTCTGATCCATTG CCTGATAACTGGGAAATGGCCTACACAGAGAAGGGGGAAGTCTACTTCATTGACCATAACACAAAGACAA CATCATGGCTGGATCCGCGACTTGCGAAAAAGGCTAAACCTCCAGAAGAGTGCAAAGAAAATGAGCTTCC ATATGGCTGGGAAAAAATCGATGATCCTATATATGGCACTTACTATGTTGACCACATAAATAGAAGAACA CAGTTTGAAAACCCTGTCCTGGAAGCAAAAAGGAAGCTACAGCAACATAACATGCCCCACACAGAACTTG GAGCAAAGCCCCTGCAGGCCCCAGGTTTCCGAGAAAAGCCACTCTTCACCCGGGATGCATCCCAGTTGAA GGGAACGTTCCTCAGCACCACCCTCAAAAAGAGCAACATGGGCTTTGGGTTTACCATCATTGGTGGAGAC GAGCCGGATGAGTTTCTACAGGTGAAAAGTGTGATCCCGGATGGGCCTGCCGCACAGGATGGGAAAATGG AGACAGGTGATGTCATTGTCTATATTAATGAAGTTTGTGTCCTTGGACACACTCATGCAGATGTTGTCAA ACTTTTCCAGTCTGTTCCTATTGGTCAGAGTGTCAACTTGGTGTTGTGTCGTGGCTACCCTTTGCCCTTT GACCCTGAAGATCCTGCTAACAGCATGGTGCCACCCCTTGCAATAATGGAGAGGCCACCTCCGGTGATGG TCAATGGAAGACATAACTATGAAACATACTTGGAATACATTTCTCGGACCTCACAGTCGGTCCCAGATAT TACAGACCGGCCACCTCATTCTTTGCACTCCATGCCAGCTGACGGCCAGCTAGATGGCACGTATCCACCA CCCGTCCATGACGACAATGTGTCTATGGCTTCGTCTGGAGCCACTCAAGCTGAACTTATGACCTTAACCA TTGTGAAAGGTGCCCAGGGATTTGGCTTTACTATTGCCGACAGTCCCACGGGACAGCGGGTGAAACAAAT CCTTGACATTCAGGGATGCCCTGGGCTGTGTGAAGGAGACCTCATTGTTGAGATCAACCAACAGAATGTA CAGAACCTGAGCCATACAGAAGTAGTGGATATACTTAAGGACTGCCCCGTTGGAAGTGAGACTTCTTTAA TCATCCATCGAGGAGGTTTCTTTTCTCCATGGAAAACTCCAAAGCCTATGATGGACCGATGGGAGAACCA AGGCAGTCCACAAACAAGTTTATCTGCTCCGGCCGTCCCACAGAACCTGCCCTTCCCACCTGCCCTTCAC AGGAGCTCCTTTCCTGATTCAACAGAGGCCTTTGACCCACGGAAGCCTGACCCATATGAGCTCTACGAGA AATCGAGAGCCATTTATGAAAGTAGGCAACAAGTGCCACCCAGGACCAGTTTTCGAATGGATTCCTCTGG TCCAGATTATAAGGAACTGGATGTTCACCTTCGGAGGATGGAGTCTGGATTTGGCTTTAGAATCCTTGGG GGAGATGAACCTGGACAGCCTATTTTGATCGGAGCCGTCATTGCCATGGGCTCAGCTGACAGAGACGGCC GTCTACACCCAGGAGATGAGCTTGTCTATGTCGATGGGATCCCAGTGGCTGGCAAGACCCACCGCTATGT CATCGACCTCATGCACCACGCGGCCCGCAATGGGCAGGTTAACCTCACTGTGAGAAGAAAGGTGCTATGT GGAGGGGAGCCCTGCCCAGAGAATGGGAGGAGTCCAGGCTCTGTATCAACTCACCACAGCTCTCCGCGCA GTGACTATGCCACCTACTCCAACAGCAACCACGCCGCCCCCAGCAGCAATGCCTCACCTCCTGAAGGCTT TGCCTCACACAGCTTGCAGACCAGTGATGTGGTCATTCACCGCAAAGAAAACGAAGGGTTTGGCTTCGTC ATCATCAGCTCTCTGAACAGGCCTGAGTCTGGAGCCACCATAACTGTGCCCCATAAAATTGGACGAATCA TTGATGGGAGCCCTGCAGATCGCTGTGCCAAACTCAAAGTGGGCGACCGTATCTTAGCAGTCAACGGCCA GTCTATCATCAACATGCCTCACGCTGACATTGTGAAGCTCATCAAGGACGCCGGTCTCAGTGTCACCCTT CGCATCATTCCTCAGGAGGAGCTCAACAGCCCAACATCAGCACCCAGTTCAGAGAAACAGAGCCCCATGG CCCAGCAGCACAGCCCTCTGGCCCAGCAGAGTCCTCTGGCCCAGCCAAGCCCCGCCACCCCCAACAGCCC AGTCGCACAGCCAGCTCCTCCCCAACCTCTCCAGCTGCAAGGACACGAAAATAGTTACAGGTCAGAAGTT AAAGCGAGGCAAGATGTGAAGCCAGACATCCGGCAGCCTCCCTTCACAGACTACAGGCAGCCCCCGCTGG ACTACAGGCAGCCCCCGGGAGGAGACTACTCACAGCCCCCACCCTTGGACTACAGGCAGCACTCTCCAGA CACCAGGCAGTACCCTCTGTCAGACTACAGGCAGCCACAGGATTTTGATTATTTCACTGTGGACATGGAG AAAGGAGCCAAAGGATTTGGATTCAGCATTCGTGGAGGAAGGGAATACAAGATGGATCTGTATGTGTTGA GATTGGCAGAGGATGGGCCAGCCATAAGGAACGGCAGGATGAGGGTAGGAGATCAGATCATTGAAATAAA TGGGGAAAGCACACGAGACATGACCCACGCCAGAGCAATAGAACTCATCAAGTCTGGAGGAAGAAGAGTG CGGCTGCTGCTGAAGAGAGGCACGGGGCAGGTCCCGGAGTATGGAATGGTACCTTCCAGCCTCTCCATGT GCATGAAAAGTGACAAGCATGGGTCCCCATATTTCTACTTACTGGGCCACCCTAAAGACACGACGAACCC CACGCCTGGAGTGCTGCCGCTGCCGCCGCCCCAGGCCTGCCGGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211649 representing BC059005 Red=Cloning site Green=Tags(s) MELEKSGALLESGTYEDNYYGTPKPPAEPAPLLNVTDQILPGATPSAEGKRKRNKSVTNMEKASIEPPEE EEEERPVVNGNGVVITPESSEHEDKSAGASGETPSQPYPAPVYSQPEELKDQMDDTKPTKPEENEDSDPL PDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRT QFENPVLEAKRKLQQHNMPHTELGAKPLQAPGFREKPLFTRDASQLKGTFLSTTLKKSNMGFGFTIIGGD EPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLGHTHADVVKLFQSVPIGQSVNLVLCRGYPLPF DPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYISRTSQSVPDITDRPPHSLHSMPADGQLDGTYPP PVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDIQGCPGLCEGDLIVEINQQNV QNLSHTEVVDILKDCPVGSETSLIIHRGGFFSPWKTPKPMMDRWENQGSPQTSLSAPAVPQNLPFPPALH RSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTSFRMDSSGPDYKELDVHLRRMESGFGFRILG GDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVIDLMHHAARNGQVNLTVRRKVLC GGEPCPENGRSPGSVSTHHSSPRSDYATYSNSNHAAPSSNASPPEGFASHSLQTSDVVIHRKENEGFGFV IISSLNRPESGATITVPHKIGRIIDGSPADRCAKLKVGDRILAVNGQSIINMPHADIVKLIKDAGLSVTL RIIPQEELNSPTSAPSSEKQSPMAQQHSPLAQQSPLAQPSPATPNSPVAQPAPPQPLQLQGHENSYRSEV KARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYSQPPPLDYRQHSPDTRQYPLSDYRQPQDFDYFTVDME KGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRV RLLLKRGTGQVPEYGMVPSSLSMCMKSDKHGSPYFYLLGHPKDTTNPTPGVLPLPPPQACRK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC059005
ORF Size	3336 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC059005, AAH59005
RefSeq Size	4375 bp
RefSeq ORF	3338 bp
Locus ID	50791
Gene Summary	Seems to act as scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth. May play a role in regulating activin-mediated signaling in neuronal cells. Enhances the ability of PTEN to suppress AKT1 activation (By similarity).[UniProtKB/Swiss-Prot Function]

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