Nos2 (BC062378) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	iNOS, NOS-II, Nos2a, i-NOS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211704 representing BC062378 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAGGAAAAGGACA TTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGCCCAACAATACAAGATGACCCTAAGAGTCA CCAAAATGGCTCCCCGCAGCTCCTCACTGGGACAGCACAGAATGTTCCAGAATCCCTGGACAAGCTGCAC GTGACTCCATCGACCCGTCCACAGTATGTGAGGATCAAAAACTGGGGCAGTGGAGAGATTTTGCATGACA CTCTTCACCACAAGGCCACATCGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCC CAAGAGTTTGACCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAG TTCATCAACCAGTATTATGGCTCCTTTAAAGAGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTG TAACAAAGGAAATAGAAACAACAGGAACCTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGAT GGCCTGGAGGAATGCCCCTCGCTGCATCGGCAGGATCCAGTGGTCCAACCTGCAGGTCTTTGACGCTCGG AACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACTTTATGCCACCAACAATGGCA ACATCAGGTCGGCCATCACTGTGTTCCCCCAGCGGACTGACGGCAAACATGACTTCAGGCTCTGGAATTC ACAGCTCATCCGGTACGCTGGCTACCAGATGCCCGATGGCACCATCAGAGGGGATGCTGCCACCTTGGAG TTCACCCAGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCT TGCAAGCTGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGA GCATCCCAAGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACATG CTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGATTG GAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGAAGTGGGCCGAAGGATGGGCCTGGA GACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGGAGATCAATGTGGCTGTGCTCCATAGT TTCCAGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGA ATGAGTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCAT CACCCCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGATCGAGCCC TGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAGAGAGATCCGATTTAGAGTCTTGG TGAAAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCT CTTTGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTC AACACCAAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAACTACTGCTGGTGG TGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGACTCTGAAGAAATCTCTGTTCATGCT TAGAGAACTCAACCACACCTTCAGGTATGCTGTGTTTGGCCTTGGCTCCAGCATGTACCCTCAGTTCTGC GCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTGGGAGCCTCTCAGCTTGCCCCAACAGGAGAAG GGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCTGGGCTGTACAAACCTTCCGGGCAGCCTGTGA GACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAACGCTTCACTTCCAATGCAACATGGGAG CCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCTCAACAGAGCCCTCAGCAGCATCCATG CAAAGAATGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGCCGCACCAC CCTCCTCGTTCAGCTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATC TTCCCAGGCAACCAGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACC AAACTGTGTGCCTGGAGGTTCTGGATGAGAGCGGCAGCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTG CTCACTCAGCCAAGCCCTCACCTACTTCCTGGACATTACGACCCCTCCCACCCAGCTGCAGCTCCACAAG CTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAGAGATTGGAGGCCTTGTGTCAGCCCTCAGAGTACA ATGACTGGAAGTTCAGCAACAACCCCACGTTCCTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCC CGCTGCCTTCCTGCTGTCGCAGCTCCCTATCTTGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGAC CACACCCCCTCGGAGGTTCACCTCACTGTGGCCGTGGTCACCTACCGCACCCGAGATGGTCAGGGTCCCC TGCACCATGGAGTCTGCAGCACTTGGATCAGGAACCTGAAGCCCCAGGACCCAGTGCCCTGCTTTGTGCG AAGTGTCAGTGGCTTCCAGCTCCCTGAGGACCCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGC ATTGCTCCCTTCCGAAGTTTCTGGCAGCAGCGGCTCCATGACTCCCAGCACAAAGGGCTCAAAGGAGGCC GCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTCTATCAGGAAGAAATGCAGGAGAT GGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGCTGCCCGGCAAACCCAAGGTCTAC GTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCCACGGGGAGCAGGGCCACC TCTACGTTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACGTTGAAGAAGCTGGTGGCCACCAA GCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGAGCCAGAAACGTTATCATGAA GATATCTTCGGTGCAGTCTTTTCCTATGGGGCAAAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGA GGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211704 representing BC062378 Red=Cloning site Green=Tags(s) MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH VTPSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIE FINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDAR NCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRTDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLE FTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANM LLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHS FQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEP WKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAF NTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFC AFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWE PQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGI FPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHK LARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQD HTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTG IAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVY VQDILQKQLANEVLSVLHGEQGHLYVCGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHE DIFGAVFSYGAKKGSALEEPKATRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC062378
ORF Size	3435 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062378, AAH62378
RefSeq Size	4112 bp
RefSeq ORF	3437 bp
Locus ID	18126
Gene Summary	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

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