Atp13a2 (BC042661) Mouse Tagged ORF Clone

CAT#: MG211743

  • TrueORF®

Atp13a2 (tGFP-tagged) - Mouse ATPase type 13A2 (cDNA clone MGC:28522 IMAGE:4192094)



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Mouse Protein / Lysate Request

CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1110012E06Rik; AA589443
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211743 representing BC042661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCAGACAGCAGCCTGCTCATGGGCAGCACGCCCCCCAGCTATGGGACCCTGACGACAGGGACGT
CAATCGATCCCCTCAGCTCCTCAGCTTCATCCGTGAGGCTCAGCGGCTACTGTGGCAGTCCATGGAGGGC
CATCGGCTATCACGCCGCGGTCTGGATGCTGGCCGGGATCCCTTGGCTGCTATTCCGCTGGAAGCCCTTG
TGGGGCGTGCGCCTGCGCCTGAAGCCATGCAGCCTGGCCCACGCCGAAACACTCGTTATAGAAATAAAAG
ACAAAGAGGGTAGCTCCAGACAGCTCTTCACGGTTCAGGTGCAGACTGAGGCTCTTGTCCAGGGCAGCCT
GGAGCTGCCCCCACAGGCCCAGGCAGAGGATGGCCGGAGCCAGGCAGCCGTGGGAGTGACACCAGAGGGC
ACATGGCAGGACACCTCCGAACTCCACAGGCAGGAAGAGGCAAAGCAGGTACTGCGCTATTATGTCCTCC
AAGGTCAACGCTACGTCTGGATGGAAACCCAGCAAGCCTTCTGCCAAGTCAGCCTGCTGGACCACAGTCG
TACCTGTGATGACGTCCACTGCTCCAGCTCCGGCCTCAGCCTCCAAGACCAAGCTACGAGGAAGACCATT
TACGGCCCCAATGTGATCAGTATTCCTGTCAAGTCCTACCTGCAGCTGCTGGCGGATGAGGCCCTGAATC
CCTACTATGGGTTCCAAGCCTTCAGCATCGCACTGTGGCTGGCCGACCACTACTACTGGTATGCTCTCTG
CATCTTCCTCATCTCAGCCATCTCCATCTGCCTGGCTCTGTACAAGACCAGAAAGCAAAGCTTGACCCTG
AGGGACATGGTCAAGCTGTCCGTGCGGGTGCAGGTGTGCCGACCCGGAGGAGAGGAAGAGTGGGTGGACT
CCAGTGAGCTGGTGCCCGGAGACTGCCTGGTGCTGCCCCAGGAGGGCGGGGTGATGCCATGCGACGCGGC
TCTGGTGGCCGGGGAGTGTGTGGTCAACGAGAGCTCCCTGACAGGGGAGAGCACCCCTGTGCTGAAGACG
GCCCTGCCAGAGGGGCCCAAGCCCTACTGCCCAGAGACCCACCGGCGGCACACCCTCTTCTGTGGCACCC
TCATTCTGCAGGCCCGAGCCTACGTGGGACCCCGTGTGCTAGCAGTGGTGACTCGGACAGGGTTCTGCAC
AGCCAAAGGGGGCCTGGTGAGCTCCATCCTACACCCAAGGCCCATCAGCTTCAAGTTCTACAAACACAGC
ATGAAGTTTGTGGCTGCGCTCTCCGTCCTGGCTCTGCTCGGCACCGTCTACAGCATCGTCATCCTCTACC
GAAACCGGGTACCTGTGAGGGAGATTGTGATCCGAGCTCTGGACCTGGTGACGGTGGTGGTGCCGCCCGC
CCTGCCTGCCGCCATGACGGTGTGCACACTCTATGCCCAGAGTCGGCTGCGCGCACAGGGCATCTTCTGC
ATCCACCCACTGCGCATCAACTTGGGGGGCAAGCTGCGGCTCGTGTGCTTTGACAAGACAGGCACCCTCA
CAGAGGACGGCTTGGACGTAATGGGCGTAGTGCCCCTAAAGGGGCAGGTGTTGCTGCCACTGGTCCCAGA
GCCCCGCCACCTGCCCCTGGGGCCCCTCCTGCGAGCACTGGCCACCTGTCATGCCCTCAGCCAGCTACAT
GACACCCCGGTGGGCGACCCCATGGATCTGAAGATGGTGGAGTCTACAGGCTGGGTCTTAGAGGAGGGTC
CAGCTGCAGGCTCAGCACCTGGGAGCCAGGTCTTGGTGGTAATGAGACCCCCACCCGGGGGCCCTCGGCA
GCAGGAAGAGCCACCAGTGCCAGTCAGCGTCCTCTGCCGCTTCCCCTTCTCCTCTGCCCTGCAGCGGATG
GATGTGGTAGTGACATGGCCAGGGGCCACCCAGCCTGAGGCCTACGTCAAAGGCTCCCCAGAGCTTGTGG
CCAGCCTCTGCAGCCCCGAGACAGTACCCAGCGACTTTTCTCAGGTGCTGCAGAGCTACACAGCTGCTGG
CTACCGAGTTGTGGCCCTGGCTGGCAAGCCACTGCCCATTGCGCCCAGCCTTGAGGCCGCTCAGCAGCTG
ACAAGGGACACTGTGGAGAGGGAACTGAGCCTCCTGGGGCTGCTGGTCATGCGGAACCTACTGAAGCCAC
AGACGGCCCCAGTTATCCAGACTCTGAGGAAGACGGGCATCCGCACCGTTATGGTGACAGGGGACAACCT
GCAGACAGCAGTCACCGTGGCCAGAGCTTGTGGCATGGTGGGCGCCCAGGAGCACCTGGCTGTCATCCAT
GCCACCCACCCCGAGCAGGGCCAGCCTGCTGCCCTTGAGTTCTTGCCAACGGAGTCCTCTGCAGTCATGA
ATGGGGCGAAGGCCACAGGCTATCCCACGGTGCCGGAACCCCAGTTCTGTCACCTGGCCCTCAGCGGGTC
CACGTTTGCAGTCCTTCGGAAGCACTTCCCCAAGCTGCTGCCCAAGGTCCTGGTGCAGGCTACCGTCTTT
GCCCGCATGGCCCCGGAGCAGAAGACAGAGCTAGTGTGCGAACTGCAGAGGCTTCAGTACTGTGTGGGCA
TGTGCGGGGATGGAGCCAACGACTGTGGGGCCCTGAAGGCGGCTGATGTGGGCATATCGCTGTCCCAGGC
GGAGGCGTCAGTGGTCTCTCCGTTCACCTCCAGCATGGCCAGTATTGAATGTGTGCCCACTGTCATCAGG
GAAGGCCGCTGCTCCCTGGATACTTCATTCAGCGTCTTCAAGTACATGGCCCTCTACAGCCTGACTCAGT
TCATCTCTGTGCTCATTCTCTACACAATCAACACCAACCTGGGAGACCTACAGTTCTTGGCCATTGACCT
GGTCATCACCACCACGGTTGCGGTGCTCATGAGCCGCACAGGCCCTGCACTGACCTTGGTGCGAGCGCGG
CCACCAGGGGCGCTGCTGAGTGTGCCTGTGCTTGGCAGTCTGCTGCTGCAGGTGGCCTTGGTGGCCGGCA
TCCAGCTGGGGGGCTACTTTTTGGTCATAGCTCAGCCCTGGTTTGTGCCTCTGAACAGAACTGTGCCCGC
TCCGGACAACCTGCCCAACTACGAGAATACGGTGGTCTTCTCTCTGTCTGGCTTCCAGTACCTCATCCTG
GCCGCAGCGGTGTCTAAGGGGGCACCCTTCCGCCAGCCACTCTACACCAACGTGCCCTTCCTGGTGGCCC
TGGCGCTCTTGGGCTCTGTCCTGGTGGGCCTCATCCTGGTCCCCGGCCTCCTGCAGGGGCCCCTAGGACT
GAGGAACATTGTGGACAGCTCCTTCAAGCTACTGCTGCTGGGTCTGGTCGCCTTCAACTTCGTCGGCGCC
TTTATGCTGGAGAGCGTGCTGGACCAGTGTCTCCCAGCCTGCCTGCGGTGGCTGCGGCCTAAACGGGCCT
CCAAGAAGCAATTCAAGCGGCTGCAACAGGAGCTTGCAGAACATCCATGGCCCACTCTGCCCGTTGGCTC
CGTGAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211743 representing BC042661
Red=Cloning site Green=Tags(s)

MSADSSLLMGSTPPSYGTLTTGTSIDPLSSSASSVRLSGYCGSPWRAIGYHAAVWMLAGIPWLLFRWKPL
WGVRLRLKPCSLAHAETLVIEIKDKEGSSRQLFTVQVQTEALVQGSLELPPQAQAEDGRSQAAVGVTPEG
TWQDTSELHRQEEAKQVLRYYVLQGQRYVWMETQQAFCQVSLLDHSRTCDDVHCSSSGLSLQDQATRKTI
YGPNVISIPVKSYLQLLADEALNPYYGFQAFSIALWLADHYYWYALCIFLISAISICLALYKTRKQSLTL
RDMVKLSVRVQVCRPGGEEEWVDSSELVPGDCLVLPQEGGVMPCDAALVAGECVVNESSLTGESTPVLKT
ALPEGPKPYCPETHRRHTLFCGTLILQARAYVGPRVLAVVTRTGFCTAKGGLVSSILHPRPISFKFYKHS
MKFVAALSVLALLGTVYSIVILYRNRVPVREIVIRALDLVTVVVPPALPAAMTVCTLYAQSRLRAQGIFC
IHPLRINLGGKLRLVCFDKTGTLTEDGLDVMGVVPLKGQVLLPLVPEPRHLPLGPLLRALATCHALSQLH
DTPVGDPMDLKMVESTGWVLEEGPAAGSAPGSQVLVVMRPPPGGPRQQEEPPVPVSVLCRFPFSSALQRM
DVVVTWPGATQPEAYVKGSPELVASLCSPETVPSDFSQVLQSYTAAGYRVVALAGKPLPIAPSLEAAQQL
TRDTVERELSLLGLLVMRNLLKPQTAPVIQTLRKTGIRTVMVTGDNLQTAVTVARACGMVGAQEHLAVIH
ATHPEQGQPAALEFLPTESSAVMNGAKATGYPTVPEPQFCHLALSGSTFAVLRKHFPKLLPKVLVQATVF
ARMAPEQKTELVCELQRLQYCVGMCGDGANDCGALKAADVGISLSQAEASVVSPFTSSMASIECVPTVIR
EGRCSLDTSFSVFKYMALYSLTQFISVLILYTINTNLGDLQFLAIDLVITTTVAVLMSRTGPALTLVRAR
PPGALLSVPVLGSLLLQVALVAGIQLGGYFLVIAQPWFVPLNRTVPAPDNLPNYENTVVFSLSGFQYLIL
AAAVSKGAPFRQPLYTNVPFLVALALLGSVLVGLILVPGLLQGPLGLRNIVDSSFKLLLLGLVAFNFVGA
FMLESVLDQCLPACLRWLRPKRASKKQFKRLQQELAEHPWPTLPVGSVR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC042661
ORF Size 3509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC042661, AAH42661
RefSeq Size 3953 bp
RefSeq ORF 3509 bp
Locus ID 74772
Gene Summary ATPase that plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Required for a proper lysosomal and mitochondrial maintenance.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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