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Prag1 (NM_172911) Mouse Tagged ORF Clone
CAT＃: MG211757
- TrueORF®
D8Ertd82e (tGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e)
ORF Plasmid: DDK
"NM_172911" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9830148H23; D8Ertd82e; mFLJ00269; NACK; Sgk223 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211757 representing NM_172911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGTGCAGCGACTTTGTGGAGCACATCTGGAAACCCGGGTCTTGCAAGAACTGCTTCTGCTTGC GAAGTGATCATCAGCCAACAGCTGGCCATCCCAAGGCTAGAGCCAACAGCCTACCCGCTGGAACTCGCCT GCCTGCCAGGCCTGAGAATTGCCGCCTAGACGACGAAGGCGTGAATGGCTTGGCCTACTCAAAGCCCACC ATTGCTGTAAAGCCCACCATGATGACTTCTGAGACCTCCGACTTGTGGACTGAAGCCAGCCTGAGCGCTG AAGTCCCAAAGGTCAATTGGAGACGAACCCCAGGCAAGCTCCTGCTCCCGAAACAGGAAGATGAGCCAAT AGTTTACCTAGGCAGCTTCCGAGGACTGCAGAAGCCTGCAAGCCCTCTTGCCTGCACAGATGGCAACTCT CGTTGTCCCCCAGCCTATACCATGGTCGGACTGCATAACCTGGAGGCCCGCGTGGATAGGAACACTGCCT TTCAACCAGTGAGCTTCCAGGAAGAGAAGGCTGGGAGGGAAGAGCTGCCCTCAGCTCACGAGAGCTTTCG CCAGAAGCTGGCTGCCTTTGCAGGGATGACATCCAGCTGCCCCAAGGGCCCCAGGCCCTGCACTTCTCCA CAGCCCCTGCGGGAATCCCTGCCCTCGGAGGATGACAGTGACCAAAGGTGCTCACCCTCAGGAGACAGCG AAGGTGGGGAGTACTGTTCCATTCTGGACTGTTGTCCAGAATCCAAAGATGCTGTGCATAGCACCGAGGG CTCTGGCAGACGTGGAGGGGACTGCTCACCCACGTGCAGGGAGCAGGGACCACGTACAAGACCAACAGAA GAGGAAAAACAGGGTCTGAGCTTCCCCAGAGAGTGCTGTGGTCAGGGATCCACAGCGAACCCGCCCCGCC TGGGCCCCAAGAAGCCATCCCTTAACTCTGAGGCCGCCAGCTCTTCCGATGGTCTGTCCTGTGGCAGCAG CCGCAGCGGGGCCAGCAGCCCCTTCGCTCCCCACCTTGAGAACGACTACTGCTCTCTTGTGAAGGAACCG GCCTCAGGGAAGCAGCAAGACCTCAGCGGCCACTTTCTCACCTCTGGCAAATGTGTGGGTCAAGCTGCGG AACTCCAGCCCGCCTCCCTTCTTAGGGACCCTGTGCAGCCTGAACCCATCTACGCCGAAAGCGCCAAGAG GAAGAAGGCAGCTCCGGGGCCTCCGAGGCCCGAGCCCAAGAAAGAGCAGGTCCCAGCTGGCCATAGCCAG GGCCAAGTATGGACAGGTGATACCTGGATTCAGAAGACACCCCCTAGCTGGAGCCAGGACAGGGAAGGCG CAAACCCTGCTCCTCAGGTGGCAACCACCATCACTGTCATCGCTGCTCACCCGGAAGAGGACCACAGGAC TATTTATCTGAGCAGCCCTGACTCTGCCGTGGGGGTGCAGTGGCCACGTGGGCCTTCGAACCAGGACTTA CAAGCCGGTGAAGAGGAGCCTTTGGTTGCGCAGGGGCTAACCTCAAGGGAGAGCCATCCACATAATGTGA CTGAAAACACGGCCAAAGAGAAGCCTGCCATTCCCCCCAAACTGTCCAAAAGCAGCCCCGGAGGGTCCCC TGTGTCTCCCGCGCCCCCACTGACTGACCACAGCGACGGGAACACCGGTGGCAGCAGCGTCGGACCCCAA CTTTTGTCTAGAGTCCCTGCTAACCTAACGTCTTCCTGCCACACCAATGGCGTTGCCACTGCTGGTGACT CTGCCAAGTGTCCCCCACCAGCCACCTCCTCTTCAGTCTTGGACCAGAGGCGGCCAAGATACCAGACTGG TGCTTGGAGCCGCCAGTGCCGGATAGAAGAGGAGGAGGAGGTGGGGCAGGAGTTGAGTCAAAGTTGGGGG AGAGAGTTGGAAAACGGTACCGCAGACCATTCAAACTCCTCTACCTGGCACCGTCTCCATCCCATCGATG GCACCTCTGGGCAGAACAGCAAAACTAACTCTGGGATGAGCAAATCGGCTTCCTTTGCGTTTGAGTTCCC AAAGGACAGAGGCAGATTGGAGGCATTCTCACCCCCTCCCCCACCTCCAAAGTCAAGGCACCTGTTAAAA ATGAACAAGAGCAGTTCTGACTTGGAAAAAGTGAGCCAGAGTTCTGCAGAAAGCCTCAGCCCGTCCTTCA GAGGGGCCCACGTCAGCTTCACCACGGGCTCCACAGATAGCCTAGCCTCGGACTCCAGGCCCTGCAGCGA TGGAGGTCCATCATATGAACCCACCCACTCACCCACCATCAGCGGGAAAAAGCTCTTCGCTCCCGTCCCT TTTCCATCAGGCTCCACAGAGGATGTGTCCCCTGGTGGTGGTCCTGCGCAGCCTCCTCCTCTGCCCCAGA AAAAGATAGTGAGCCGTGCCGCCTCTTCCCCTGATGGCTTCTTCTGGACCCAAGGTTCCCCTAAGCCACG GACGGCAAGTCCCAAGCTGAACCTAAGCCATTCAGAAACCAATGTTTGTGCCCATGACGAGCCTCCTTTT AACTGTTCCTTGAACTCTGGAAACCGTTCCCACCATGTCTTCTCCTCTTCTGAGCCTTTGGGGAAAGCTT TCAAAGGCAATGCTCCCTGGGCCCCAGCTCTGGGGCTGGCGAACAGCAAAGGCGGCTGTGGGAGCCCCAG TCTCCAGTGCAGAGCGGCCACCTCCACGTCATCATCCCAGCTCAGCGTGTCCAGCCAGGCCTCCTCCAGC AGCACCCAGCTTCAGCTGCACAGCCTGCTGAGTAGCATCAGCAGCAAGGAGGGCACCTATGCCAAGCTTG GGGGCCTCTACACACAATCCCTGGCCCGCCTAGTGACCAAGTGTGAAGATCTCTTCATGGGTGGCCAGAA AAAGGAACTGCGCTTCAATGAGAATTACTGGTCACTCTTCAAGCTGACGTGCAACAAACCTTGCTGTGAC TCGGGGGATGCAATTTACTACTGTGCCACCTGCTCTGAGGACCCAGGCAGCATCTATGCTGTGAAAATCT GCAAAACCCCAGAACCCAAATCAGCCTCATACTGCAGCCCATCTGTGCCGGTGCATTTCAACATCCAGCA AGACTGCGGCCACTTTGTGGCCTCTGTGCCCTCCAGCATGCTTGCCTCCCCTGACACATCCAGCAAAGAC ACCGCGCCTGCCGTATCCCCACAGCCCCCTGCCCAGGAGCAGGACTGTGTGGTGGTCATCACCCGGGAGG TACCGCACCAGACTGCCTCCGACTTTGTGCGGGACTCTATGGCCAGCCACCGGGCGGAGCCTGAGGTTTA CGAGCGCCGGGTGTGCTTCCTGCTTCTGCAGCTCTGCAACGGGCTGGAGCACCTGAAGGAACACGGGATC ATCCACCGGGACCTGTGTCTGGAGAACCTGCTGCTGGCGCACTGTAACCCTCAGAGCTCCCCCGGGCCCT CTGCCACTCCTACCGTGCCCACCACCACATCCAGGTGCCCTTCTGCCGCGCCCGCAGCCACCACTGCCTG CCAGGGAGGGCCTGGTGAGAAGCAGCTGCCCAGGCTCATCATCAGCAACTTCCTGAAGGCCAAGCAAAAG CCAGGAGGCACCACCAACCTGCAACAGAAGAAGAGCCAGGCCCGGCTGGCTCCCGAGATCGTGTCTGCCT CCCAGTACCGCAAGTTTGATGAGTTCCAGACGGGCATCCTCATCTATGAGCTGCTCCACCAGCCCAACCC ATTCGAGGTGCGAGCCCAGCTCCGGGAGCGCGACTACCGGCGGGAGGACTTACCCCCGCTGCCCACACTG TCCCTCTACTCTCCCGGCCTGCAGCAACTCGCCCATCTACTGCTTGAGGCTGATCCCATCAAGCGCATCC GCATAGGTGAAGCTAAGCGGGTGCTGCAGTGCTTGCTGTGGGGGCCACGGCGGGAGCTGGTGGAGCAGCC GTGCACGTCAGAGGAGGTACTGTGTAACACTCTGCACAACTGGATCGACATGAAGCGAGCACTGATGATG ATGAAGTTTGCTGAGAAGGCGGTGGACCGAAGGCGGGGTGTGGAACTGGAGGACTGGCTTTGCTGCCAGT ACTTGGCCTCGGCAGAGCCAGGGGCCCTCTTGCAGTCCCTGAAGCTCCTACAACTTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211757 representing NM_172911
Red=Cloning site Green=Tags(s) MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAGTRLPARPENCRLDDEGVNGLAYSKPT IAVKPTMMTSETSDLWTEASLSAEVPKVNWRRTPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNS RCPPAYTMVGLHNLEARVDRNTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSP QPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRTRPTE EEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSPFAPHLENDYCSLVKEP ASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYAESAKRKKAAPGPPRPEPKKEQVPAGHSQ GQVWTGDTWIQKTPPSWSQDREGANPAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDL QAGEEEPLVAQGLTSRESHPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQ LLSRVPANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELSQSWG RELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGRLEAFSPPPPPPKSRHLLK MNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRPCSDGGPSYEPTHSPTISGKKLFAPVP FPSGSTEDVSPGGGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPF NCSLNSGNRSHHVFSSSEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSS STQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKLTCNKPCCD SGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLASPDTSSKD TAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGI IHRDLCLENLLLAHCNPQSSPGPSATPTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQK PGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTL SLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHNWIDMKRALMM MKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172911 |
| ORF Size | 4119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172911.3, NP_766499.2 |
| RefSeq Size | 4780 bp |
| RefSeq ORF | 4122 bp |
| Locus ID | 244418 |
| UniProt ID | Q571I4 |
| Gene Summary | Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224352 | D8Ertd82e (untagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), (10ug) |
CNY 17,580.00 |
|
| MR211757 | D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
CNY 9,392.00 |
|
| MR211757L3 | Lenti ORF clone of D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
CNY 12,730.00 |
|
| MR211757L4 | Lenti ORF clone of D8Ertd82e (mGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) |
CNY 12,730.00 |
