Nid2 (BC054746) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Ly111, entactin-2, NID-2, nidogen-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211968 representing BC054746 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGGGACCCGACAGCCGGGTGGCTAACACCGCCATCACCGCTATCGCTGCTGGTGATGCTGTTGC TGCTGTCGCGAGTCGGGGCTCTGCGTCCCGACGAGCTCTTTCCTTACGGGGAGTCGTGGGGAGACCAGCT GCTGCCGGAGGGCGACGACGAAAGCTCAGCAGCTGTGAAGCTGGCAATACCCCTGCGTTTCTACGATGCC CAGTTCAGCAGCCTCTACGTGGGCACCAACGGCATCATCTCCACCCAGGACTTCCCCAGGGAGACTCAAT ATGTAGATGATGATTTTCCTACTGACTTCCCAGCCATCGCCCCTTTCCTGGCTGACATCGACACTAGTCA CAGCAGGGGCCGGATCCTGTACCGCGAGGACACCTCCGGAGCTGTGCTGAGCCTGGCTGCCCGCTACGTG CGCACTGGCTTCCCGCTGTCGGGGTCCAGCTTCACCCCCACCCACGCCTTCCTGGCCACCTGGGAGCACG TGGGCGCCTACGAGGAGGTCAGTCGCGGGGCTGCGCCGTCAGGAGAGCTGAACACTTTCCAGGCTGTTCT GGCATCCGATGAATCTGATACCTACGCCCTCTTTCTCTACCCTGCCAATGGCCTTCAGTTCTTTGGAACC CGCCCCAAAGAGTCCTACAATGTCCAGCTGCAGCTTCCCGCTAGGGTGGGCTTCTGCCGAGGGGAAGCGG ACGACCTGAAGAGAGAAGCACTGTATTTCAGCCTGACTAACACCGAGCAGTCTGTGAAGAATCTCTACCA GCTAAGCAACCTGGGGATTCCTGGAGTGTGGGCTTTTCACATCGGCAGCAGGTTTGCCCTGGACAATGTC CGTCCAGCCACAGTTGGAGGTGACCCTTCCACAGCCCGTTCCTCAGCACTGGAGCACCCCTTCAGCCATG CTGCAGCCCTGGAGAGCTACACCGAGGACAGTTTCCATTACTACGATGAGAATGAAGAGGATGTTGAGTA CCCACCAGTTGAACCGGGGGAGGCCCCGGAAGGCCACAGCAGAATTGATGTGTCTTTCAATTCAAAGGCT GATCCAGGCCTTGTGGACGTCGGCACCTCATCTCCAGGTTCCGACCGCGCTTCTCCTTGGCCATATCCAG CACCTGGTAACTGGCCATCCTACCGGGAAACAGAATCGGCTTCTTTGGACCCTCAAACCAAACAGGGGCG GCCGGTGGGAGAGGGAGAGGTTCTGGATTTCAGGGACCCAGCGGAGCTTTTGGACCAGATGGGTACCAGA GCCCCAGCTCCTCCAGAGGCAGATGCAGCTCTCCTAACTCCAGTCAACGAAGACCTTGGGGGCAGAAACA CCCAGTCCTATCCAGAGGCAGGGCCAGTGCCTTCAGAGCCAGATGTTCCTGTCCCTCCTCTGGAAGGAGA AGTCCTTCCTCATTACCCCGAGTCTGGTCACGTTCCGCCCCTGAGAGGAGGGAAGTATGTGATAGGACTG GAGGACCACGTGGGATCTAATGATCAAGTCTTCACGTATAATGGCGCCAACCTGGAAACCTGCGAACACA GCCACGGCCGGTGCTCCCAGCATGCCTTCTGCACCGACTACACCACTGGCTTTTGCTGCCACTGCCAGTC GAGGTTTTACGGAAACGGGAAGCACTGCCTGCCAGAAGGGGCACCTCACCGAGTGAATGGGAAAGTGAGT GGTCGCCTCCGAGTGGGTCACATTCCTGTGCACTTCACTGACGTGGATCTACACGCTTACATCGTGGGCA ATGATGGCAGAGCCTACACTGCCATCAGCCATGTCCCACAGCCGGCAGCCCAGGCCCTGCTCCCTGTCCT GCCCATTGGAGGTCTGTTTGGCTGGCTCTTTGCTTTGGAGAAGCCAGGCTCTGAGAATGGCTTCAGCCTC ACAGGTGCTACCTTTGTCCATGACGTGGAAGTCACTTTTCACCCTGGAGAGGAGAGGGTTCGTATCACTC AAACTGCCGAGGGGCTTGACCCAGAAAACTATCTGAGCATTAAGACCAACATTGAAGGCCAGGTGCCCTT CATCCCAGCGAATTTCACAGCCCACATTACTCCGTACAAAGAGTTTTATCATTACAGGGACTCAGTCGTG ACGTCCTCCAGTTCCCGAAGTTTCTCTCTCACTTCTGGTTCTATTAACCAAACGTGGTCCTACCACATTG ACCAGAACATCACTTACCAGGCGTGCAGGCACGCCCCCAGACACCTGGCCATCCCTGCCACCCAGCAGCT GACTGTCGACCGGGCCTTTGCTTTGTACAGTGAGGATGAGGGCGTGCTGAGGTTTGCTGTGACCAATCAG ATTGGCCCTGTTGAAGTGGACTCAGCCCCTGTTGGAGTGAATCCTTGCTACGACGGAAGCCACACCTGTG ACACAACAGCAAGGTGCCACCCGGGAACAGGTGTAGACTACACCTGCGAGTGCACGCCTGGGTTCCAGGG TGACGGACGGAGCTGTGTGGATGTCAATGAGTGTGCCACTGGCTTCCACCGCTGTGGCCCTAATTCTGTG TGTGTCAACTTGGTGGGAAGCTACAGGTGTGAATGCCGCAGTGGCTATGAATTTGCAGATGACCAGCACA CTTGTATCTTGATCGCCCCACCTCCCAACCCTTGCCTCGATGGCAGTCACACCTGTGCCCCTGAGGGGCA AGCCAGGTGCATTCACCATGGAGGCAGCTCATTCAGTTGTGCCTGCCTGCCAGGCTTCATTGGCACTGGG CATCAGTGTTCTGATGTTGATGAATGTGCGGAAAACCGATGTCATGAGGCAGCTATCTGCTACAATACCC CCGGGTCCTTCTCCTGCCGTTGCCAGCCTGGGTATCGAGGGGACGGGTTTCACTGCACCTCTGACACGGT TCCGGAAGATTCCATCTCAGGACTGAAGCCCTGTGAATACCAGCAGCGCTATGCTCAGACACAGCACGCC TACCCTGGGTCACGGATCCACATCCCCCAGTGTGATGACCAGGGAAACTTCGTGCCACTGCAGTGCCACG GCAGCACTGGCTTCTGCTGGTGTGTGGACCGAAATGGCCACGAAGTCCCTGGCACGCAGACTCCACCCGG CTCCACCCCGCCCCACTGTGGACCACCTCCAGAGCCCACCCAGAGGCCTCGGACTGTCTGTGAGCGTTGG CGGGAAAGCCTGCTGGAACACTACGGAGGCACACCCAGGGATGACCAGTATGTGCCCCAGTGTGATGACC TGGGCCACTTCATCCCCCTGCAGTGCCATGGAAAGAGTGATTTCTGTTGGTGTGTGGACAAGGATGGCAG AGAGCTACAGGGCACGCGCTCACAGCCAGGCACCAGGCCTGCATGCATACCCACCGTCGCTCCACCCGTG GTCCGGCCCACACCCCGGCCCGATGTGACTCCTCCATCTGTGGGCACCTTCCTGCTCTATGCCCAGGGCC AACAGATTGGCCACTTGCCCCTCAATGGCAGCAGGCTTCAGAAGGACGCAGCCCGGACCCTGCTGTCACT GCATGGCTCCATAGTTGTGGGGATTGACTATGACTGCCGGGAGAGGATGGTCTACTGGACAGACGTTGCT GGTCGGACCATCAGCCGTGCCAGCTTGGAGGCAGGAGCCGAACCCGAGACCATCATTACCTCAGGTTTGA TAAGCCCGGAAGGACTTGCCATCGACCACTTCCGTAGAACAATGTACTGGACAGATAGTGGCCTGGATAA GATAGAGCGGGCCGAACTGGATGGTTCCGAGCGGAAAGTCCTCTTCCACACAGATCTGGTGAATCCACGA GCCATCACTGTGGATCCAATCCGAGGCAACTTGTACTGGACAGACTGGAATAGAGAAGCTCCTAAAATTG AAACATCATCTTTAGATGGCGAAAACAGAAGGATTCTGATCAACAAAGATATTGGATTACCCAATGGATT GACCTTTGACCCCTTCTCCAAACTGCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGTACACTA CCCGATGGAACTGGACGACGTGTCATCCAAAACCACCTCAATTACCCCTTCAGCATCGTCAGCTATGCAG ATCACTTCTACCACACGGACTGGCGGAGGGATGGTGTTATATCAGTGAATAAGGACAGTGGCCAGTTTAC TGATGAGTTTCTCCCTGAGCAGCGGTCTCATCTCTATGGGATCACTGCAGTCTACCCGTACTGTCCAACA GGAAGAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211968 representing BC054746 Red=Cloning site Green=Tags(s) MFRDPTAGWLTPPSPLSLLVMLLLLSRVGALRPDELFPYGESWGDQLLPEGDDESSAAVKLAIPLRFYDA QFSSLYVGTNGIISTQDFPRETQYVDDDFPTDFPAIAPFLADIDTSHSRGRILYREDTSGAVLSLAARYV RTGFPLSGSSFTPTHAFLATWEHVGAYEEVSRGAAPSGELNTFQAVLASDESDTYALFLYPANGLQFFGT RPKESYNVQLQLPARVGFCRGEADDLKREALYFSLTNTEQSVKNLYQLSNLGIPGVWAFHIGSRFALDNV RPATVGGDPSTARSSALEHPFSHAAALESYTEDSFHYYDENEEDVEYPPVEPGEAPEGHSRIDVSFNSKA DPGLVDVGTSSPGSDRASPWPYPAPGNWPSYRETESASLDPQTKQGRPVGEGEVLDFRDPAELLDQMGTR APAPPEADAALLTPVNEDLGGRNTQSYPEAGPVPSEPDVPVPPLEGEVLPHYPESGHVPPLRGGKYVIGL EDHVGSNDQVFTYNGANLETCEHSHGRCSQHAFCTDYTTGFCCHCQSRFYGNGKHCLPEGAPHRVNGKVS GRLRVGHIPVHFTDVDLHAYIVGNDGRAYTAISHVPQPAAQALLPVLPIGGLFGWLFALEKPGSENGFSL TGATFVHDVEVTFHPGEERVRITQTAEGLDPENYLSIKTNIEGQVPFIPANFTAHITPYKEFYHYRDSVV TSSSSRSFSLTSGSINQTWSYHIDQNITYQACRHAPRHLAIPATQQLTVDRAFALYSEDEGVLRFAVTNQ IGPVEVDSAPVGVNPCYDGSHTCDTTARCHPGTGVDYTCECTPGFQGDGRSCVDVNECATGFHRCGPNSV CVNLVGSYRCECRSGYEFADDQHTCILIAPPPNPCLDGSHTCAPEGQARCIHHGGSSFSCACLPGFIGTG HQCSDVDECAENRCHEAAICYNTPGSFSCRCQPGYRGDGFHCTSDTVPEDSISGLKPCEYQQRYAQTQHA YPGSRIHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERW RESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGTRSQPGTRPACIPTVAPPV VRPTPRPDVTPPSVGTFLLYAQGQQIGHLPLNGSRLQKDAARTLLSLHGSIVVGIDYDCRERMVYWTDVA GRTISRASLEAGAEPETIITSGLISPEGLAIDHFRRTMYWTDSGLDKIERAELDGSERKVLFHTDLVNPR AITVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINKDIGLPNGLTFDPFSKLLCWADAGTKKLECTL PDGTGRRVIQNHLNYPFSIVSYADHFYHTDWRRDGVISVNKDSGQFTDEFLPEQRSHLYGITAVYPYCPT GRK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054746
ORF Size	4211 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054746, AAH54746
RefSeq Size	4885 bp
RefSeq ORF	4211 bp
Locus ID	18074
Gene Summary	Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.[UniProtKB/Swiss-Prot Function]

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