Neo1 (BC054540) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610028H22Rik; AI327052; D930014N22Rik; Igdcc2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212000 representing BC054540 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCGCGAAGCCGGGCGACTCCTCTGCACCTCCTCCTCCCGGCGCTGCTGTCCGCCACCGC CGCTGCTGCTGTTGCTGCCGCTGCTGCTGCTGCTCGGACGCCCGGCGTCCGGCGCCGCGGCCACGAAGAG CGGCTCCCCGCCGCAGTCCGCAGGAGCCAGTGTTCGAACATTCACTCCGTTTTATTTTCTGGTGGAGCCA GTAGACACCCTCTCAGTTAGAGGCTCTTCTGTTATATTAAATTGCTCGGCATATTCTGAGCCCTCTCCAA ACATTGAATGGAAGAAAGATGGGACTTTTTTAAACTTAGAATCAGATGATCGACGCCAGCTACTCCCAGA TGGATCTTTATTCATCAGCAACGTGGTGCATTCCAAACACAATAAGCCTGACGAAGGTTTCTATCAGTGT GTAGCCACTGTGGATAATCTTGGAACCATTGTCAGCAGAACAGCCAAGCTCACAGTAGCAGGTCTTCCAA GATTTACCAGCCAACCAGAACCTTCTTCAGTCTATGTTGGAAACAGTGCAATTCTGAATTGTGAAGTTAA TGCAGATTTGGTCCCATTTGTTAGGTGGGAACAGAATCGACAGCCCCTTCTTCTAGATGACAGGATTGTC AAACTTCCAAGTGGAACACTGGTTATCAGCAATGCTACTGAAGGAGATGGGGGACTCTACCGCTGCATTG TTGAAAGTGGTGGGCCACCAAAGTTTAGTGACGAAGCTGAATTGAAAGTTCTTCAAGATCCTGAGGAAAT TGTAGACTTGGTATTTCTGATGCGACCATCTTCTATGATGAAAGTCACTGGTCAGAGTGCAGTGTTGCCA TGTGTTGTCTCAGGGCTTCCTGCTCCAGTTGTTAGATGGATGAAAAACGAAGAAGTGCTTGACACAGAAA GCTCTGGCAGGTTGGTCTTGCTAGCAGGAGGTTGCTTGGAGATCAGTGATGTCACTGAGGATGATGCTGG GACTTATTTTTGCATAGCTGATAATGGAAATAAGACAGTTGAAGCTCAGGCGGAGCTTACTGTGCAAGTG CCACCTGGATTCCTGAAACAACCTGCTAACATATATGCTCACGAATCCATGGACATTGTATTTGAATGTG AAGTCACTGGGAAGCCAACTCCAACTGTGAAGTGGGTCAAGAATGGGGATGTGGTTATCCCCAGTGATTA CTTTAAAATTGTAAAGGAACATAATCTTCAAGTTTTGGGTCTGGTGAAATCAGATGAAGGGTTCTATCAA TGCATTGCTGAGAATGATGTTGGAAATGCACAAGCTGGAGCCCAGCTGATAATCCTTGAGCATGATGTTG CCATCCCAACATTACCTCCCACTTCACTGACCAGTGCCACTACTGACCATCTAGCACCAGCCACAACGGG ACCATTACCTTCAGCTCCTCGAGACGTCGTGGCCTCCCTGGTCTCTACTCGCTTCATTAAATTGACATGG CGTACACCTGCATCAGACCCTCATGGAGACAATCTCACCTACTCTGTGTTCTACACCAAGGAAGGGGTTG CTAGGGAGCGTGTTGAGAATACCAGCCAGCCAGGAGAGATGCAGGTGACTATTCAAAACTTGATGCCAGC AACTGTGTACATCTTCAAAGTTATGGCTCAAAATAAGCATGGCTCTGGAGAAAGTTCAGCTCCTCTTCGA GTAGAGACACAGCCTGAGGTTCAGCTCCCTGGCCCAGCACCTAATATCCGTGCTTATGCAACGTCACCTA CTTCTATCACTGTCACCTGGGAAACACCGTTATCTGGCAATGGGGAAATTCAAAATTACAAATTGTACTA CATGGAAAAAGGAACTGATAAAGAACAGGATATTGATGTTTCAAGTCACTCCTACACCATTAATGGACTG AAGAAATACACAGAATACAGTTTCCGAGTGGTGGCCTACAATAAACATGGTCCTGGAGTTTCTACACAAG ATGTTGCTGTTCGAACATTATCAGATGTTCCCAGTGCTGCTCCTCAGAATCTGTCCTTAGAAGTGAGAAA TTCAAAGAGTATAGTGATCCACTGGCAGCCCCCTTCCTCAACCACACAAAATGGGCAGATAACTGGCTAC AAGATTCGATATCGAAAGGCCTCCCGAAAAAGTGATGTCACTGAGACCTTGGTAACTGGGACACAGCTGT CTCAGCTGATTGAAGGTCTTGATCGGGGGACAGAATATAACTTCCGAGTCGCTGCTCTCACAGTCAATGG TACAGGTCCAGCAACTGATTGGCTGTCTGCTGAAACTTTTGAAAGCGACCTAGATGAAACTCGTGTTCCT GAAGTGCCCAGCTCTCTTCATGTCCGTCCGCTCGTCACTAGCATTGTAGTGAGCTGGACTCCTCCAGAGA ACCAGAACATTGTGGTCCGAGGTTATGCCATCGGTTACGGCATTGGCAGCCCTCATGCCCAGACCATCAA AGTGGACTATAAACAACGTTATTACACCATCGAAAACTTGGATCCAAGCTCTCATTACGTGATTACCTTG AAAGCATTTAACAATGTTGGCGAAGGCATCCCCCTTTATGAGAGTGCTGTGACCAGACCTCACACAGTGC CAGATCCCACTCCCATGATGCCACCAGTGGGAGTTCAGGCTTCCATTCTGAGTCACGACACCATAAGGAT TACCTGGGCAGACAACTCCCTGCCCAAACACCAGAAGATTACAGACTCCCGCTACTACACAGTCCGGTGG AAGACCAACATCCCAGCAAACACGAAGTACAAGAATGCAAATGCAACGACGTTAAGCTATTTGGTTACTG GTTTAAAGCCAAATACGCTCTATGAGTTCTCTGTGATGGTGACCAAAGGCAGAAGGTCAAGCACGTGGAG TATGACAGCTCATGGCGCTACCTTTGAATTAGTTCCTACTTCTCCACCTAAGGATGTGACAGTTGTGAGT AAGGAAGGAAAACCTAGAACCATCATAGTGAATTGGCAGCCTCCCTCTGAAGCTAACGGCAAGATTACAG GTTACATCATCTATTACAGCACGGATGTGAATGCAGAGATACATGACTGGGTTATTGAACCAGTTGTGGG AAACAGACTGACTCACCAGATTCAAGAGTTAACACTTGATACGCCATACTACTTCAAAATCCAGGCCCGG AACTCAAAGGGCATGGGGCCCATGTCTGAAGCTGTACAGTTCAGAACACCTAAAGCCTTAGGGTCAGCAG GAAAAGGAAGCCGACTACCAGACCTGGGATCTGACTACAAACCTCCAATGAGTGGCAGCAACAGCCCTCA CGGGAGCCCCACCTCCCCTCTGGACAGCAACATGCTGCTGGTCATCATTGTCTCTGTTGGCGTCATCACT ATCGTGGTGGTTGTGGTCATTGCTGTCTTTTGTACCCGGCGCACCACCTCTCACCAGAAGAAGAAACGAG CTGCGTGCAAATCAGTGAATGGCTCCCATAAGTACAAGGGCAATTGCAAAGATGTGAAGCCTCCAGACCT ATGGATCCATCACGAGAGACTAGAGTTGAAGCCTATTGACAAGTCTCCAGATCCTAACCCTGTCATGACT GATACTCCAATCCCTCGAAACTCTCAAGATATCACACCAGTGGACAATTCCATGGATAGCAATATCCATC AAAGGCGGAATTCATACAGAGGGCATGAGTCAGAGGACAGCATGTCTACACTGGCTGGAAGGAGGGGAAT GAGACCAAAAATGATGATGCCCTTTGACTCTCAGCCACCTCAGCCTGTGATTAGTGCCCATCCCATCCAT TCCCTCGATAACCCTCACCATCATTTCCACTCCAGCAGCCTCGCTTCTCCAGCCCGCAGTCATCTCTACC ACCCAAGCAGCCCATGGCCCATTGGCACATCCATGTCCCTTTCAGACAGGGCCAATTCCACAGAATCTGT TCGAAATACCCCCAGCACGGACACCATGCCAGCGTCCTCGTCTCAGACGTGCTGCACTGACCATCAGGAC CCTGAGGGTGCTACTAGCTCCTCTTACTTGGCCAGCTCCCAAGAGGAAGACTCAGGCCAGAGTCTTCCCA CAGCCCATGTCCGCCCTTCCCACCCTCTGAAGAGCTTCGCTGTGCCAGCAATCCCACCCCCAGGACCTCC TCTCTATGATCCTGCACTGCCAAGCACACCATTACTGTCCCAGCAAGCTCTGAACCATCACATTCACTCA GTGAAAACAGCCTCCATCGGGACGTTAGGAAGGAGCCGGCCTCCTATGCCAGTGGTTGTTCCGAGTGCCC CTGAAGTACAGGAGACCACCAGGATGCTGGAAGACTCCGAGAGTAGCTATGAACCAGATGAGCTGACCAA AGAGATGGCCCACCTGGAAGGACTAATGAAGGACCTAAATGCCATCACAACAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212000 representing BC054540 Red=Cloning site Green=Tags(s) MAAEREAGRLLCTSSSRRCCPPPPLLLLLPLLLLLGRPASGAAATKSGSPPQSAGASVRTFTPFYFLVEP VDTLSVRGSSVILNCSAYSEPSPNIEWKKDGTFLNLESDDRRQLLPDGSLFISNVVHSKHNKPDEGFYQC VATVDNLGTIVSRTAKLTVAGLPRFTSQPEPSSVYVGNSAILNCEVNADLVPFVRWEQNRQPLLLDDRIV KLPSGTLVISNATEGDGGLYRCIVESGGPPKFSDEAELKVLQDPEEIVDLVFLMRPSSMMKVTGQSAVLP CVVSGLPAPVVRWMKNEEVLDTESSGRLVLLAGGCLEISDVTEDDAGTYFCIADNGNKTVEAQAELTVQV PPGFLKQPANIYAHESMDIVFECEVTGKPTPTVKWVKNGDVVIPSDYFKIVKEHNLQVLGLVKSDEGFYQ CIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATTGPLPSAPRDVVASLVSTRFIKLTW RTPASDPHGDNLTYSVFYTKEGVARERVENTSQPGEMQVTIQNLMPATVYIFKVMAQNKHGSGESSAPLR VETQPEVQLPGPAPNIRAYATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDIDVSSHSYTINGL KKYTEYSFRVVAYNKHGPGVSTQDVAVRTLSDVPSAAPQNLSLEVRNSKSIVIHWQPPSSTTQNGQITGY KIRYRKASRKSDVTETLVTGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVP EVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITL KAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRW KTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGATFELVPTSPPKDVTVVS KEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQAR NSKGMGPMSEAVQFRTPKALGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVIT IVVVVVIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNCKDVKPPDLWIHHERLELKPIDKSPDPNPVMT DTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIH SLDNPHHHFHSSSLASPARSHLYHPSSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQD PEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPPGPPLYDPALPSTPLLSQQALNHHIHS VKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054540
ORF Size	4395 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054540, AAH54540
RefSeq Size	5443 bp
RefSeq ORF	4397 bp
Locus ID	18007

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.