Shprh (BC055003) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610103K11Rik; AA450458; AU024614; BC006883; D230017O13Rik; E130018M05
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212063 representing BC055003 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTCGATGAAGAAAGGCAACAGCAGCTTCACTGGA ACATGCATGAGGACCTGAGGAGTGAGCCGCTCACCATGACGGTCGGGGAACAGGCCTGTTCAGATGCAGA CTCTTCTTCTGATTGCATCATCATCGATGAAGGCCCTCCTGAGAGTGCACTTCACAGAGATAAGAAGAGG CGTTCAGAGACTGTGAGCGTCTTAGAGGCCACCGAGGAAGAGACCCGTTTGTCTGTGACTTTGAATGTTA CAGTTTCTCCCTATCGTGTGGATAATTCTTGGAAAGCATTTCTGGGAGATTTTGCTCTTCAGCTTCTCCC CAAAGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACATTGAGTCCTTCAGAGTCAAGCAGTCAG TTCCTGATCTATGTTCATTCAGAATGTAAAAATGTAGAGAAACAAGAAAATGTTCTTGAAGGATCAGCCG GTGTTTGTAGCAAGGGAATCCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCT ACAGAAGAGAAGAGGAATAAAACTCTACCAGAGACCAGACGGCACTCACACAATCAAGGTTGGAATTTAC ATTTTGGAAGCTGGCCTAACAAGACTAGACTTCATGAGTGATGCAGGTTCCAGAATGAAAAAGTTCAATC AGCTCATGAAGAGAGTGATGGAAAAGTTACATAATTTCATTATTCCAGATGTGCTGGAAGAAGAGGAGGA GGGTTCAGAGAGCGAGCCCGAGGGTCAGGATATTGATGAGCTCTATCACTTTGTGAAGCAAACACACCAG CAAGAAACACGGTCAGTCCAAGTGGACGTCCAGCACCCTGCGTTGATTCCGGTCCTGAGACCTTACCAGA GAGAGGCAGTCAACTGGATGCTGCAGCAAGAGCAGTTCAGAAGCGCTCCTCCTGCTGATAACTCCCTGCA CTTCTTATGGCGAGAAATTGTTACACCTGATGGCTTGAAACTCTACTATAATCCATATACAGGCTGCATC ATTCGAGATTTCCCACATGCGGGTCCACAGTTGCTTGGTGGAATCTTAGCAGATGAGATGGGTCTTGGAA AGACAGTGGAGGTTCTGGCTCTGATTCTGACACATACCCGACAAGACGTCAAGCAGGACGCTCTCACCCT TCCTGAGGGAAAAGTGGTAAATTATTTCATCCCAACTCACTGTCCTAGAGAAAAAGTGAAAAATAGAGAA ATCCAGGATACAGAATATGAACCAAAAGAGAAAGTTCACTGCCCTCCTACACGTGTGATGATCCTGACAG CTGTGAAAGAAATGAATGGGAAGAAAGGAGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCATATT CAGATATGATGTTCAGCGGAACCGGGGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTT GTGAAACAGATCAAAGGCCATGGTTTCTCCGGGACTTTTACACTGGGGAAGAATTACAAAGAAGATGTAT TTGATAAAACAAAAAAGCAGGCAGTAGGGAGTCCAAGGAAAATTGAGAAGGAATTGAGGAAATCAGTGAA CAAGGATGCAGATTCTGAGTACCTGCCATCAAACACTTCTGATGACGATGAGCCTTACTATTATTACTGT AAGGCGGGAAAAAGCCGGAGTAAGTTGAAGAAGCCTGCTTTACTAACTAAAAAGGGAAAGGGTCAGTCTG TTCATCTGGATTCCCAAGGTGATGCTCCAGCTGCTGGAGTCTGTGCAAGTACTGATGTTCATGTGTCAGA AAATACATGTGTCTCTGAAGACAAGCAAACCCAAGAAGCAAAAGACTGTGCTGAGTCGCCAAACCCTGCT GCCGAGGAGTTGGCACAGTCTAACACTTCAAGTCCTTGTGAAACCTCTGATTACCGCTTTGAGTGTATAT GTGGTGAATTTGATCAAATTGGCCACAAGCCAAGAGTTCAATGTCTGAAGTGTCACCTGTGGCAGCATGC AAAGTGTGTGAATTATGAGGAGAAAAACCTGAAGGTTAAGCCTTTTTACTGCCCCCACTGTCTTGTTGCG ATGGAGCCAGTATCAACAAGAGCAACTCTGATCATCTCTCCGAGCTCCATTTGCCACCAGTGGGTGGATG AAATCAATCGGCACGTGAGGTCATCATCTCTCCGAGTTCTGGTATATCAAGGAGTGAAGAAACACGGCTT CTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTATGATGTTCTTCGTTCAGAACTA AACTATGTCAATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGAGGAACCAGAAGCGCTATATGG CCATTCCCAGCCCCTTGGTAGCGGTGGAATGGTGGAGGATCTGCCTTGATGAAGCTCAGATGGTCGAGTG TCCTACAGTAAAAGCCGCGGAAATGGCCCAGCGTTTGAGCGGGATTAATCGGTGGTGCATCAGTGGCACT CCAGTTCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTGGAATTGAACCTTATTGTGTCA AACACTGGTGGATTCGGCTTCTCTATCATCCATACTGCAAGAAAAATCCTCAGCACCTGTACAGTTTTAT TGCCAAGATAATGTGGAGGTCTGCAAAGAAAGATGTCATTGACCAAATCCAGATTCCACCTCAGACTGAA GAAATGCACTGGCTCCATTTTTCCCCGGTGGAGAGGCATTTCTATCACCGCCAGCACGAGGTGTGCTGCC AGGATGCCATAGTGAAGCTCAGGAAGATCTCTGACTGGGCCCTGAAGCTCAGCAGCTTGGACAGGAGAAC TGTCTCCTCCATCCTCTACCCACTGCTGAGGCTCCGGCAGGCCTGCTGCCATCCACAGGCTGTTCGTGGG GAATTCTTGCCACTCCAGAAGAGCACCATGACAATGGAAGAGTTGCTGACATCTCTGCAGAAGAAATGTG GAACGGAATGTGAAGAGGCCCACCGACAACTAGTTTGTGCCCTTAACGGCTTAGCAGGCATTCACATCAT TAAAGGTGAATATGCCTTGGCAGCGGAGCTCTACAGAGAAGTATTACGTTCATCTGAGGAGCACAAAGGA AAACTCAAAACTGATTCCCTTCAAAGACTGCATGCCACACATAACTTAATGGAGCTGTTGGGAGCCAAAC ACCCAGGAATACCTCCCACCTTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTGCGAGAGCACTA TATGAGTAAGTGTAACACAGAAGTTGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGCAGTCCATTAGG GAGCTCCAAAGGAAGATTCATTCTAATTCTCCTTGGTGGCTGAATGTAATCCATAGAGCAATGGAATTTT CTGTTGATGAGGAACTTGTTCAGCGGGTGCGAAATGAAATAAGCAGTAACTACAAGCAACAAACTGACAA GCTGTCTATGTCAGAGAAGTTCCGTGACTGCAGAGGTCTTCAGTTCTTACTTACAACACAAATGGAAGAG CTCCATAAGTTCCAGAAGCTGGTGAGGGAGGCTGTGAAGAAGCTGGAGAAGCCTCCATCCCGGGAGGTGA TTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGCGTCTTTTGTAAAGC TGATGAATTGTTCACAGAGTATGAATCAAAGCTATTTTTTAACACAGTCAAAGGCCAGACTGCAATCTTT GAAGAAATGATAGAAGATGAAGAAGGACTTGTAGATGATAGAGTACCTACCACTACCAGAGGTCTGTGGG CCGTAAGTGAGACGGAGCGATCTATGAAAGCAATATTATCATTTGCAAGATCACATAGGTTTGATGTTGA ATATGTAGATGAAGGAAGTGTTTCAATGGATCTCTTTGAGGCATGGAAGAAGGAATATAAGTTACTTCAT GAATACTGGATGACTCTGAGAAATCGTGTATCTGCTGTTGATGAACTTGCAATGGCTACAGAGCGACTAA GAGTGCGCCATCCCAAAGAGCCAAAGCCAAACCCACCCGTCCATCACATCATTGAACCACACGAGGTTGA ACAGAATCGTATAAAACTGGTGAATGATAAAGCTGTTGCTACATCACAGCTTCAGAAGAAACTTGGACAG CTTCTTTACCTAACGAATTTGGAGAAGTCTCAAGATAAAACATCAGGAGGTATTAATCCAGAGCCTTGTC CAATCTGTGCTCGACAGCTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTCTGTAATGAATG CACTTCGATTATAATTGAACAGTACAGTGTGGGGTCTCATCGAAGTTCCATCAAGTGTGCTATCTGCCGC CAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACCTCAGAGAAGGCAAACCAAGAGGATGATATCC CTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATACAACTTAGAGA TCCAGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTATTAGATATTATTTCAAAAGCTCTCACG GACAACAACATGGAATTTACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTCTCAGCGTTTAAAT ACGATCCCCACATCAATATTCTGCTGCTGCCCCTGCACACAGGCTCTAATGGATTAACTATCATTGAAGC CACCCATGTCCTCCTGGTGGAGCCCATACTGAACCCTGCCCATGAGCTGCAGGCCATTGGGAGAGTGCAC CGAATTGGACAGACCAAACCTACTATTGTACATAGATTCTTAATTAAAGCAACAATAGAAGAACGGATGC AGGCAATGCTAAAAACTGCTGAGAGGAGCCACACAAGCTCATCCGGAAAGCATTCTGAAGCCTCTGTGTT GACTGTGGCTGGCCTGGCTGACCTGTTTACCAAGGAGAACGAGGAGCTTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212063 representing BC055003 Red=Cloning site Green=Tags(s) MSSRRKRAPPMKVDEERQQQLHWNMHEDLRSEPLTMTVGEQACSDADSSSDCIIIDEGPPESALHRDKKR RSETVSVLEATEEETRLSVTLNVTVSPYRVDNSWKAFLGDFALQLLPKESLVEHFSERTFTLSPSESSSQ FLIYVHSECKNVEKQENVLEGSAGVCSKGIRVESSFSSDMLQDLAWLQKRRGIKLYQRPDGTHTIKVGIY ILEAGLTRLDFMSDAGSRMKKFNQLMKRVMEKLHNFIIPDVLEEEEEGSESEPEGQDIDELYHFVKQTHQ QETRSVQVDVQHPALIPVLRPYQREAVNWMLQQEQFRSAPPADNSLHFLWREIVTPDGLKLYYNPYTGCI IRDFPHAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPTHCPREKVKNRE IQDTEYEPKEKVHCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIFRYDVQRNRGLLKRMLKCLIFEGL VKQIKGHGFSGTFTLGKNYKEDVFDKTKKQAVGSPRKIEKELRKSVNKDADSEYLPSNTSDDDEPYYYYC KAGKSRSKLKKPALLTKKGKGQSVHLDSQGDAPAAGVCASTDVHVSENTCVSEDKQTQEAKDCAESPNPA AEELAQSNTSSPCETSDYRFECICGEFDQIGHKPRVQCLKCHLWQHAKCVNYEEKNLKVKPFYCPHCLVA MEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKHGFLQPHFLAEQDIVIITYDVLRSEL NYVNIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGT PVQRGLEDLFGLVVFLGIEPYCVKHWWIRLLYHPYCKKNPQHLYSFIAKIMWRSAKKDVIDQIQIPPQTE EMHWLHFSPVERHFYHRQHEVCCQDAIVKLRKISDWALKLSSLDRRTVSSILYPLLRLRQACCHPQAVRG EFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKG KLKTDSLQRLHATHNLMELLGAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQSIR ELQRKIHSNSPWWLNVIHRAMEFSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRDCRGLQFLLTTQMEE LHKFQKLVREAVKKLEKPPSREVIESATVCHLRPARLPLNCCVFCKADELFTEYESKLFFNTVKGQTAIF EEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAILSFARSHRFDVEYVDEGSVSMDLFEAWKKEYKLLH EYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEVEQNRIKLVNDKAVATSQLQKKLGQ LLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSSIKCAICR QTTSHKEVSYVFTSEKANQEDDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALT DNNMEFTQISRIKTFQENLSAFKYDPHINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVH RIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTSSSGKHSEASVLTVAGLADLFTKENEELE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC055003
ORF Size	5024 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055003, AAH55003
RefSeq Size	6977 bp
RefSeq ORF	5024 bp
Locus ID	268281
Gene Summary	E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.[UniProtKB/Swiss-Prot Function]

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