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Polr1a (NM_009088) Mouse Tagged ORF Clone
CAT＃: MG212069
- TrueORF®
Polr1a (tGFP-tagged) - Mouse RNA polymerase 1-4 (Rpo1-4)
ORF Plasmid: DDK
"NM_009088" in other vectors (2)
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CNY 20,810.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 194kDa; 3010014K16Rik; mRPA1; RPA194; Rpo1-4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212069 representing NM_009088
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGCTGGCCTCCAAGCACACACCATGGAGGCGGCTTCAGGGCATTTCCTTCGGAATGTATTCGGCCGAAG AGCTCAAGAAGTTAAGTGTTAAATCCATTACAAATCCTCGATACGTGGACTACCTGGGAAACCCTTCAGC AAATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCGCTACCTGCGTGCAGGAC TTCAACAACTGCTCTGGACACCTGGGCCACATTGATCTCCCGCTCACAGTCTACAACCCTTTCCTCTTTG ATAAACTCTACCTGCTGCTTCGAGGCTCCTGTTTGAGTTGCCACATGCTGACTTGTCCTCGGGCTGCAAT TTACCTGTTGATCTCCCAGCTGAGGGTCCTGGAAGTTGGAGCCCTGCAAGCAGTCTACGAGCTTGAGCGA ATTCTCAGCAGGTTTCTGGAAGAAACTGGAGATCCTTCCGCCTTCGAAATTCAAGAAGAGTTAGAAGAAT ACACAAGCAAAATTCTGCAGAACAACCTCTTGGGGTCCCAGGGCACACATGTGAAAAATGTGTGCGAGAG CAGAAGCAAACTTGTCGCTCAGTTCTGGAAGACACACATGGCTGCGAAGCAGTGTCCACACTGCAAGACG GGACGGTCGGTGGTTCGAAAGGAGCACAATAGTAAACTAATCATCACATACCCAGCCACGGTGCACAAGA AATCAGACCAGGAGGGCACAGAACTCCCAGAAGGAGTGCCGGAAGCTCCAGGAATTGACAAGGCTCAGAT GGGGAAACGAGGGTACCTGACCCCAAGCAGCGCCCAAGAACACCTTTTCGCCATTTGGAAGAATGAAGGA TTCTTTCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT TCTTTCTAGACTTCATAGTCGTACCGCCATCCAGGTACCGTCCCGTCAATCGCCTGGGTGACCAGATGTT CACTAATGGCCAGACAGTAAACTTGCAAGCTGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCG TTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAACTCACCATAGACAAGGAAAATGACTCCT CAGTGGCTATCGACCGATCCTTCCTGGGTTTGCTTCCAGGCCCGTCTCTCACAGATAAACTTTACAACAT TTGGATTCGCCTTCAGAGCCACGTCAATATTGTGTTTGACAGTGAGATGGACAAATTGATGTTGGAAAAG TACCCTGGCATAAGACAGATCCTAGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG TTGACTACGCAGCCCGCTCGGTCATCTGCCCAGACATGTACATCAACACCAATGAGATCGGAATCCCCAT GGTATTTGCCACAAAACTGACTTACCCTCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC TGAGCTCAGTGGATGCTGCGCAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCAGCCACAGGGGCTCC GAAGCCTCAGGGGACAAAAGTTGTGTGCCGGCATGTGAAAAATGGGGACATCCTCCTCCTGAACCGACAG CCTACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCGCATTCTGCCTGAAGAAAAAGTCCTACGCC TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTTGATGGAGATGAGATGAACGCCCACTTCCCCCA GAGTGAGTTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGATCAGCAGTATCTTGTTCCCAAGGAT GGCCAGCCATTGGCAGGACTGATCCAAGATCACATGGTTTCAGGTGCAAACATGACCATTCGGGGATGCT TTTTCACCCGGGAACAGTACATGGAACTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT CTTTCCTCCCGCCATCCTGAAGCCCTTCCCACTGTGGACAGGAAAACAGGTTGTGTCGACCCTCCTCATA AACATAATTCCAGAGGACTACGCCCCCCTGAACTTATCTGGAAAGGCAAAAATTGGCAGCAAAGCCTGGG TGAAGGAAAAGCCACGGCCTATCCCTGACTTTGACCCGGACTCCATGTGTGAGTCCCAGGTGATCATCAG GGAAGGAGAGCTGCTCTGCGGAGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC TGCTGCTATGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCCTAGCCCGCCTCTTCACTG CCTACCTCCAGCTCTATAGAGGTTTCACCTTGGGTGTAGAAGACATTTTGGTTAAGCCAAATGCAGATGT GGTGAGACAGCGTATCATTGAAGAATCTACCCAGTGTGGACCCCAGGCTGTCAAGGCTGCATTGAGCCTG CCAGAAACTGCATCATGTGATGAAATCCAAGGGAAATGGCAGGATGCCCATCTGAGCAAAGACCAGAGGG ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG TATGCCTCTTGGCTTGCACAGACAGTTCCCCGAAAACAACCTGCAGATGATGGTGCAGTCAGGAGCCAAA GGGTCAACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAATTGGAAGGTCGGAGACCCC CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCCAGGGCTGGTGGCTT TGTCACTGGAAGGTTTCTTACCGGTATCAGGCCTCCTGAGTTCTTCTTCCACTGCATGGCAGGACGAGAA GGCCTGGTGGACACTGCTGTCAAAACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG AGGGGCTGGTCATCCAGTACGATCTGACGGTCCGTGACAGCGATGGCAGCGTGGTCCAGTTCCTGTATGG GGAGGACGGCCTGGACATCCCCAAGACACAGTTCCTACAGCCCAAGCAGTTCCCCTTTCTGGCCGGCAAT TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTATCCAGAGCAGATCCCCAGAAAGTGCTCG GCCACATCAAAGCCATTAAAAAATGGCATCACAAGCATTCCGGTGCCCTGCTGAGGAAAGGCGCCTTCCT CAGTTTTTCCCAGAAGATCCAGGCAGCTGTGAAAGCTTTGAACCTAAAGGGCAGTATCCAGAATGGCCGC AGCCCCGAGACCCAGCAGATGTTACAGATGTGGTATGACTTGGATGAAGAGAGCCGATGGAAATACCAGA AGAGGGCTGCCCCTTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCTACTTTGCATCTGTGTC TGAAACATTTGAAAAGAAGATTGATGACTTCAGTCAGGAATGGGCAGCTCAAGCAGAGAGGAGTTACAAG AAATCAGAGCTTTCCCTCGACAGGCTGAGAACCTTACTGCAGCTGAAGTGGCAGCGCTCACTGTGTGACC CAGGCGAGGCTGTTGGCCTCCTGGCTGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG GTGGCCAGTGCCAACATCAAAACGCCCATGATGAGTGTGCCTGTGTTCGACACCAAGAAAGCATTGAAGA AAGTGAAGAGTCTAAAGAAGAGGCTCACCAGAGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA GGAGTCCTTCTGTATGGGAGAAAAACGGAATAAGTTCCAGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAAAAACAAAGCATCAGCCTTCAGAAATGTGAATTCGAG GAGAGCTACACAGAAGGACCTCAATGACACTGAGGACTCGGGGAGGAGTCAGAGAGAGGAGGAAAGGGAT GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGCGATGCAGACGCTTCTGACACCAAAC GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGAGAGGAGGAAGAGGAGGAGGA GGTACAAGAAGAAGGGAACATCAAAGGCGACGGTGTCCACCAGGGCCATGAGCCAGATGAGGAAGAACAT TTAGGCCTCGAGGAGGAGGAGTCATCCCAGAAGCCTCCCCGCCGCCACTCCAGGCCTCAGGGAGCTGAGG CCATAAAACGCCGGATCCAGGCTGTACGGGAGTCCTACTCGTTCATCGAGGACTACCAGTATGACACAGA GGAGAGCCTGTGGTGTCAGGTGACTGTGAAGCTCCCACTGATGAAGATTAATTTTGACATGAGCTCCTTG GTTGTGTCCTTGGCCCACAAAGCCATTGTCTATACGACCAAGGGCATCACTCGGTGCCTCCTGAACGAAA CGACCAACAGTAAGAATGAGAAGGAGCTTGTACTGAACACGGAGGGAATCAACCTTCCCGAGCTGTTCAA GTACTCCGAGATACTGGACCTGCGCCGCCTCTACTCCAATGACATCCACGCCATGGCCAATACTTATGGC ATCGAGGCTGCGTTGCGGGTGATAGAGAAGGAGATTAAAGATGTGTTTGCTGTGTATGGCATTGCAGTTG ATCCTCGCCATCTCTCCCTGGTGGCTGATTACATGTGTTTTGAGGGGGTTTATAAGCCACTCAATCGCTT TGGGATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACATTTGAAACCAGCTTCCAGTTTCTGAAACAG GCCACCATGATGGGATCCCATGATGAACTCAAGTCACCTTCCGCCTGCCTCGTGGTGGGAAAGGTTGTCA AAGGCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGA ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212069 representing NM_009088
Red=Cloning site Green=Tags(s) MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDYLGNPSANGLYDLALGPADSKEVCATCVQD FNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELER ILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKTHMAAKQCPHCKT GRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEG FFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPVNRLGDQMFTNGQTVNLQAVMKDAVLIRKLLA LMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEK YPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQA VINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQ PTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKD GQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLI NIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVH CCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSL PETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAK GSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGRE GLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLAGN YEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLSFSQKIQAAVKALNLKGSIQNGR SPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWRPDIYFASVSETFEKKIDDFSQEWAAQAERSYK KSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILM VASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLP HAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKKNKASAFRNVNSRRATQKDLNDTEDSGRSQREEERD EEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEH LGLEEEESSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSL VVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYG IEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQ ATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR TRPLE - GFP Tag - V |
| Restriction Sites |
AscI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009088 |
| ORF Size | 5151 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009088.3, NP_033114.3 |
| RefSeq Size | 6145 bp |
| RefSeq ORF | 5154 bp |
| Locus ID | 20019 |
| UniProt ID | O35134 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).[UniProtKB/Swiss-Prot Function] |
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