Polr1a (NM_009088) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	194kDa; 3010014K16Rik; mRPA1; RPA194; Rpo1-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224866 representing NM_009088 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCTCCAAGCACACACCATGGAGGCGGCTTCAGGGCATTTCCTTCGGAATGTATTCGGCCGAAG AGCTCAAGAAGTTAAGTGTTAAATCCATTACAAATCCTCGATACGTGGACTACCTGGGAAACCCTTCAGC AAATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCGCTACCTGCGTGCAGGAC TTCAACAACTGCTCTGGACACCTGGGCCACATTGATCTCCCGCTCACAGTCTACAACCCTTTCCTCTTTG ATAAACTCTACCTGCTGCTTCGAGGCTCCTGTTTGAGTTGCCACATGCTGACTTGTCCTCGGGCTGCAAT TTACCTGTTGATCTCCCAGCTGAGGGTCCTGGAAGTTGGAGCCCTGCAAGCAGTCTACGAGCTTGAGCGA ATTCTCAGCAGGTTTCTGGAAGAAACTGGAGATCCTTCCGCCTTCGAAATTCAAGAAGAGTTAGAAGAAT ACACAAGCAAAATTCTGCAGAACAACCTCTTGGGGTCCCAGGGCACACATGTGAAAAATGTGTGCGAGAG CAGAAGCAAACTTGTCGCTCAGTTCTGGAAGACACACATGGCTGCGAAGCAGTGTCCACACTGCAAGACG GGACGGTCGGTGGTTCGAAAGGAGCACAATAGTAAACTAATCATCACATACCCAGCCACGGTGCACAAGA AATCAGACCAGGAGGGCACAGAACTCCCAGAAGGAGTGCCGGAAGCTCCAGGAATTGACAAGGCTCAGAT GGGGAAACGAGGGTACCTGACCCCAAGCAGCGCCCAAGAACACCTTTTCGCCATTTGGAAGAATGAAGGA TTCTTTCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT TCTTTCTAGACTTCATAGTCGTACCGCCATCCAGGTACCGTCCCGTCAATCGCCTGGGTGACCAGATGTT CACTAATGGCCAGACAGTAAACTTGCAAGCTGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCG TTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAACTCACCATAGACAAGGAAAATGACTCCT CAGTGGCTATCGACCGATCCTTCCTGGGTTTGCTTCCAGGCCCGTCTCTCACAGATAAACTTTACAACAT TTGGATTCGCCTTCAGAGCCACGTCAATATTGTGTTTGACAGTGAGATGGACAAATTGATGTTGGAAAAG TACCCTGGCATAAGACAGATCCTAGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG TTGACTACGCAGCCCGCTCGGTCATCTGCCCAGACATGTACATCAACACCAATGAGATCGGAATCCCCAT GGTATTTGCCACAAAACTGACTTACCCTCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC TGAGCTCAGTGGATGCTGCGCAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCAGCCACAGGGGCTCC GAAGCCTCAGGGGACAAAAGTTGTGTGCCGGCATGTGAAAAATGGGGACATCCTCCTCCTGAACCGACAG CCTACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCGCATTCTGCCTGAAGAAAAAGTCCTACGCC TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTTGATGGAGATGAGATGAACGCCCACTTCCCCCA GAGTGAGTTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGATCAGCAGTATCTTGTTCCCAAGGAT GGCCAGCCATTGGCAGGACTGATCCAAGATCACATGGTTTCAGGTGCAAACATGACCATTCGGGGATGCT TTTTCACCCGGGAACAGTACATGGAACTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT CTTTCCTCCCGCCATCCTGAAGCCCTTCCCACTGTGGACAGGAAAACAGGTTGTGTCGACCCTCCTCATA AACATAATTCCAGAGGACTACGCCCCCCTGAACTTATCTGGAAAGGCAAAAATTGGCAGCAAAGCCTGGG TGAAGGAAAAGCCACGGCCTATCCCTGACTTTGACCCGGACTCCATGTGTGAGTCCCAGGTGATCATCAG GGAAGGAGAGCTGCTCTGCGGAGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC TGCTGCTATGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCCTAGCCCGCCTCTTCACTG CCTACCTCCAGCTCTATAGAGGTTTCACCTTGGGTGTAGAAGACATTTTGGTTAAGCCAAATGCAGATGT GGTGAGACAGCGTATCATTGAAGAATCTACCCAGTGTGGACCCCAGGCTGTCAAGGCTGCATTGAGCCTG CCAGAAACTGCATCATGTGATGAAATCCAAGGGAAATGGCAGGATGCCCATCTGAGCAAAGACCAGAGGG ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG TATGCCTCTTGGCTTGCACAGACAGTTCCCCGAAAACAACCTGCAGATGATGGTGCAGTCAGGAGCCAAA GGGTCAACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAATTGGAAGGTCGGAGACCCC CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCCAGGGCTGGTGGCTT TGTCACTGGAAGGTTTCTTACCGGTATCAGGCCTCCTGAGTTCTTCTTCCACTGCATGGCAGGACGAGAA GGCCTGGTGGACACTGCTGTCAAAACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG AGGGGCTGGTCATCCAGTACGATCTGACGGTCCGTGACAGCGATGGCAGCGTGGTCCAGTTCCTGTATGG GGAGGACGGCCTGGACATCCCCAAGACACAGTTCCTACAGCCCAAGCAGTTCCCCTTTCTGGCCGGCAAT TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTATCCAGAGCAGATCCCCAGAAAGTGCTCG GCCACATCAAAGCCATTAAAAAATGGCATCACAAGCATTCCGGTGCCCTGCTGAGGAAAGGCGCCTTCCT CAGTTTTTCCCAGAAGATCCAGGCAGCTGTGAAAGCTTTGAACCTAAAGGGCAGTATCCAGAATGGCCGC AGCCCCGAGACCCAGCAGATGTTACAGATGTGGTATGACTTGGATGAAGAGAGCCGATGGAAATACCAGA AGAGGGCTGCCCCTTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCTACTTTGCATCTGTGTC TGAAACATTTGAAAAGAAGATTGATGACTTCAGTCAGGAATGGGCAGCTCAAGCAGAGAGGAGTTACAAG AAATCAGAGCTTTCCCTCGACAGGCTGAGAACCTTACTGCAGCTGAAGTGGCAGCGCTCACTGTGTGACC CAGGCGAGGCTGTTGGCCTCCTGGCTGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG GTGGCCAGTGCCAACATCAAAACGCCCATGATGAGTGTGCCTGTGTTCGACACCAAGAAAGCATTGAAGA AAGTGAAGAGTCTAAAGAAGAGGCTCACCAGAGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA GGAGTCCTTCTGTATGGGAGAAAAACGGAATAAGTTCCAGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAAAAACAAAGCATCAGCCTTCAGAAATGTGAATTCGAG GAGAGCTACACAGAAGGACCTCAATGACACTGAGGACTCGGGGAGGAGTCAGAGAGAGGAGGAAAGGGAT GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGCGATGCAGACGCTTCTGACACCAAAC GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGAGAGGAGGAAGAGGAGGAGGA GGTACAAGAAGAAGGGAACATCAAAGGCGACGGTGTCCACCAGGGCCATGAGCCAGATGAGGAAGAACAT TTAGGCCTCGAGGAGGAGGAGTCATCCCAGAAGCCTCCCCGCCGCCACTCCAGGCCTCAGGGAGCTGAGG CCATAAAACGCCGGATCCAGGCTGTACGGGAGTCCTACTCGTTCATCGAGGACTACCAGTATGACACAGA GGAGAGCCTGTGGTGTCAGGTGACTGTGAAGCTCCCACTGATGAAGATTAATTTTGACATGAGCTCCTTG GTTGTGTCCTTGGCCCACAAAGCCATTGTCTATACGACCAAGGGCATCACTCGGTGCCTCCTGAACGAAA CGACCAACAGTAAGAATGAGAAGGAGCTTGTACTGAACACGGAGGGAATCAACCTTCCCGAGCTGTTCAA GTACTCCGAGATACTGGACCTGCGCCGCCTCTACTCCAATGACATCCACGCCATGGCCAATACTTATGGC ATCGAGGCTGCGTTGCGGGTGATAGAGAAGGAGATTAAAGATGTGTTTGCTGTGTATGGCATTGCAGTTG ATCCTCGCCATCTCTCCCTGGTGGCTGATTACATGTGTTTTGAGGGGGTTTATAAGCCACTCAATCGCTT TGGGATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACATTTGAAACCAGCTTCCAGTTTCTGAAACAG GCCACCATGATGGGATCCCATGATGAACTCAAGTCACCTTCCGCCTGCCTCGTGGTGGGAAAGGTTGTCA AAGGCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGATAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_009088
Insert Size	5154 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009088.3, NP_033114.3
RefSeq Size	6145 bp
RefSeq ORF	5154 bp
Locus ID	20019
UniProt ID	O35134
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).[UniProtKB/Swiss-Prot Function]

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