Cacna1g (BC057399) Mouse Tagged ORF Clone

CAT#: MG212118

  • TrueORF®

Cacna1g (tGFP-tagged) - Mouse calcium channel, voltage-dependent, T type, alpha 1G subunit (cDNA clone MGC:62312 IMAGE:6410519)



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Mouse Protein / Lysate Request

CNY 24,700.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms a1G, [a]1G, alpha-1G, Cav3.1d
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212118 representing BC057399
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAGGAAGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCACGCAGCTCAACG
ACCTGTCTGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
GGCGGAGGGGCTGCCCTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGCATGCTGGTTATTCTCCTCA
ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAACGCTGCCGGATCCT
GCAGGCCTTCGACGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTCGCTTTGGGT
ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTTATCGTCATTGCTGGGA
TGCTGGAGTACTCGCTGGACCTGCAGAATGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGATACCTTGCCTATG
CTGGGCAATGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTTGGTGTTCAGCTGTGGG
CAGGGCTACTTCGAAACCGATGCTTCCTCCCTGAGAATTTCAGCCTCCCGCTGAGTGTGGACTTGGAGCC
TTACTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAACGGCATGCGA
TCCTGCAGGAGCGTGCCCACACTGCGCGGGGAAGGCGGTGGTGGTCCACCCTGCGGTCTGGACTATGAGG
CCTATAACAGTTCCAGCAATACCACCTGTGTCAACTGGAACCAATACTATACCAACTGCTCTGCAGGCGA
GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
ATCACACTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAACTTCATCT
ACTTCATTCTTCTCATCATCTTCTCTGAGACCAAGCAACGGGAGAGTCAGCTGATGCGGGAGCAGCGTGT
ACGATTCCTGTCCAATGCTAGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTTCTC
AAGTACCTGGTGTACATCCTCCGCAAAGCAGCCCGCAGGCTGGCCCAGGTCTCTAGGGCTGTAGGCGTGC
GGGCTGGGTTGCTCAGCAGCCCAGTGGTCCGTGGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCTCTCG
TTCACACCGTCGTCTGTCTGTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTG
GGTAATGGGACGCTCAGAGTTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCCCGCT
GGCTCATGCTGCCACCACCCTCTACCCCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACA
CAGCTTCTACCATGCTGACTGCCACTTGGAGCCAGTCCGTTGCCAGGCGCCCCCTCCCAGGTCCCCATCG
GAGGCATCTGGCAGGACTGTGGGTAGTGGGAAGGTATACCCCACTGTGCATACCAGCCCTCCACCAGAGA
TGCTGAAGGATAAGGCACTAGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACAT
CCCACCTGGGCCCTTCAGCTCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCC
TGCAAAATCTCCAGCCCTTGCTCCAAGGCAGACAGTGGAGCCTGTGGGCCGGACAGTTGTCCCTACTGTG
CCCGGACAGGAGCAGGGGAGCCAGAGTCCGCTGACCATGAAATGCCTGACTCAGACAGTGAGGCTGTGTA
TGAGTTCACACAGGACGCTCAGCACAGCGACCTCCGGGATCCCCACAGACGGCGACGGCCGAGCCTGGGC
CCAGATGCAGAGCCTAGTTCTGTGCTGGCCTTCTGGAGGCTGATCTGCGACACATTCCGGAAGATCGTAG
ATAGCAAATACTTTGGCCGGGGAATCATGATCGCTATCCTGGTCAATACCCTCAGCATGGGCATCGAGTA
CCACGAGCAGCCCGAGGAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCC
TTGGAGATGCTGCTGAAGCTGCTCGTCTACGGTCCCTTCGGCTACATTAAGAATCCCTACAACATCTTTG
ATGGCGTCATTGTGGTCATCAGCGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCG
GACCTTCCGCCTGATGCGGGTGCTGAAGCTAGTTCGCTTCCTGCCGGCACTGCAGCGGCAGCTCGTGGTG
CTCATGAAGACCATGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCA
TCCTGGGCATGCACCTTTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAA
GAATTTTGACTCCCTGCTCTGGGCCATTGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAA
GTCCTTTACAACGGCATGGCCTCCACGTCATCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTG
GCAACTACGTGCTCTTTAACCTGCTTGTCGCCATTCTCGTGGAGGGTTTCCAGGCAGAGGGAGATGCCAC
CAAGTCTGAGTCAGAGCCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGGAAGAAGCGCTTG
GCCCTGGTGGCCTTGGGAGAACACTCGGAACTACGAAAGAGCCTTTTGCCACCTCTCATCATCCACACAG
CTGCTACACCGATGTCACTGCCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCG
CCGCACCAGTAGCAGTGGGTCCGCTGAGCCTGGAACTGCTCATCATGAGATGAAATCACCGCCAAGTGCC
CGAAGCTCCCCGCACAGTCCCTGGAGCGCAGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCC
TGGGCCGGGCCCCCAGCCTGAAGCGTAGGAGCCCAAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGG
TCAGGAGAGCCAGGATGAGGAGGAGAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGATCATCGC
CACAGGGGTTCCTTGGAACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACCCTGCAGGTGCCCGGGC
TTCATCGAACAGCCAGCGGTCGGAGCTCTGCCTCTGAACACCAAGACTGTAATGGCAAGTCGGCTTCAGG
GCGTTTGGCCCGCACCCTGCGGGCTGATGACCCCCCACTGGATGGGGATGATGGCGATGATGAGGGCAAC
CTGAGCAAAGGGGAACGCTTACGAGCCTGGGTCCGAGCCCGGCTCCCTGCCTGTTGCCGAGAGCGAGATT
CCTGGTCTGCCTATATCTTCCCTCCCCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAA
GATGTTTGACCATGTGGTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAA
ATTGACCCCCACAGCGCTGAACGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTCCTGG
CTGAAATGACAGTGAAGGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTG
GAACGTGCTGGACGGCTTGCTGGTGCTCATCTCTGTCATCGACATCCTGGTGTCCATGGTCTCTGACAGC
GGCACCAAGATTCTCGGCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTACGTCCACTCAGGGTCATCA
GCCGGGCCCAGGGGCTGAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGT
GGTCATCTGCTGTGCCTTCTTCATCATTTTTGGAATTCTTGGGGTGCAGCTCTTCAAAGGGAAGTTCTTC
GTGTGTCAGGGTGAGGACACCAGGAACATCACTAACAAGTCCGACTGTGCTGAGGCCAGTTACCGGTGGG
TCCGGCACAAGTACAACTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGA
TGGCTGGGTTGACATCATGTATGATGGACTGGATGCTGTGGGAGTGGACCAGCAGCCCATCATGAACCAC
AACCCTTGGATGCTGCTCTACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTCGTCCTGAACATGTTTG
TGGGCGTGGTGGTGGAGAACTTCCATAAGTGCAGGCAGCACCAGGAGGAGGAGGAGGCGCGGCGGCGGGA
GGAGAAGCGACTAAAGAGGCTGGAGAAAAAGAGAAGGAATCTAATGTTGGACGATGTAATTGCTTCCGGC
AGCTCAGCCAGCGCTGCGTCAGAAGCCCAGTGCAAACCCTACTACTCTGACTACTCGCGCTTCCGGCTCC
TCGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGGTGTCATCGGGCTGAATGTGGT
CACGATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCTCTGAAGATCTGCAACTACATC
TTTACCGTCATCTTTGTCTTGGAGTCAGTATTCAAACTTGTGGCCTTCGGCTTCCGCCGGTTCTTCCAGG
ACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCATGGGCATCACGCTGGAAGAGATTGA
GGTCAATGCTTCACTGCCCATCAACCCCACCATCATCCGTATCATGAGGGTGCTCCGCATTGCTCGAGTT
CTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACACGGTGATGCAGGCCCTGCCCCAGG
TGGGGAACCTGGGACTTCTCTTCATGCTATTATTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTGG
AGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGCCGGCATGCCACCTTTAGGAACTTTGGT
ATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGAATGGTATTATGAAGGACACCCTCC
GGGACTGTGACCAGGAGTCCACCTGCTACAACACCGTCATCTCACCCATCTACTTCGTGTCCTTCGTGCT
GACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCCGTGCTGATGAAGCACCTGGAAGAGAGCAACAAA
GAGGCCAAGGAGGAGGCGGAGTTGGAGGCGGAGCTGGAGCTAGAGATGAAGACACTCAGCCCGCAGCCCC
ACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCTGGGGTGGAAGGTGTCAATAGCCCTGACAGCCCTAAGCC
TGGGGCTCCACACACCACGGCCCACATTGGAGCAGCCTCTTCAGGCTTCTCCCTTGAGCACCCCACGATG
GTACCTCACACTGAGGAGGGGCCAGTCCCCCTAGGACCAGACCTGCTGACTGTGAGGAAGTCTGGTGTCA
GCCGGACACACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAGCACTGCCGAGAGATCCCTAGG
ACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCTGTTCACTCCCAACCAGCAGAC
ACCAGCTGCATCCTACAGCTTCCCAAAGATGCACACTATCTGCTCCAGCCTCATGGGGCTCCCACCTGGG
GCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAGGCCTCTCAGGCGCCAGGCAGC
AATAAGGACTGACTCCCTGGACGTGCAGGGCCTGGGTAGCCGGGAAGACCTGTTGTCAGAGGTGAGTGGG
CCCTCCTGCCCTCTGACCCGCTCCTCATCCTTCTGGGGCGGGTCGAGCATCCAGGTGCAGCAGCGCTCCG
GCAGCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCCAGGCCTGGAACCCAGCTGGGC
CAAGGACCCTCAAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTGAGCTGGATTTCAGGAGACCTC
CTGCCCAGCAGTCAGGAAGAACCCCTGTCCCCACGGGACTTGAAAAAATGCTACAGTGTAGAGGCCCAGA
GCTGCCGGCGCAGGCCTGGGTCCTGGCTAGACGAACAGAGGAGACACTCCATCGCTGTCAGCTGCCTGGA
CAGCGGCTCCCAGCCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGCCAACCTCTTGGGGGCCCTGGG
AGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCCCGGAGAGCCAGGGCCCTCGGC
CCCCATGCAGTCCTGGCGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGACTCGAAGGATCCCTCGGCCTC
CAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGATGCGCTGAGTCTCTCTGGTTTGTCT
TCTGACCCAACAGACCTGGATCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212118 representing BC057399
Red=Cloning site Green=Tags(s)

MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR
SCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
ITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELL
KYLVYILRKAARRLAQVSRAVGVRAGLLSSPVVRGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHL
GNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPS
EASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSS
CKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLG
PDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFA
LEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVV
LMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNK
VLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRL
ALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSA
RSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHR
HRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGN
LSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPK
IDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDS
GTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFF
VCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNH
NPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASG
SSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYI
FTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARV
LKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFG
MAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNK
EAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTM
VPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPAD
TSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSG
PSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDL
LPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPG
SRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLS
SDPTDLDP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057399
ORF Size 6746 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057399, AAH57399
RefSeq Size 7527 bp
RefSeq ORF 6746 bp
Locus ID 12291
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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