Cacna1g (BC057399) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	a1G, [a]1G, alpha-1G, Cav3.1d
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212118 representing BC057399 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGGAAGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCACGCAGCTCAACG ACCTGTCTGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA GGCGGAGGGGCTGCCCTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGCATGCTGGTTATTCTCCTCA ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAACGCTGCCGGATCCT GCAGGCCTTCGACGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTCGCTTTGGGT ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTTATCGTCATTGCTGGGA TGCTGGAGTACTCGCTGGACCTGCAGAATGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGATACCTTGCCTATG CTGGGCAATGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTTGGTGTTCAGCTGTGGG CAGGGCTACTTCGAAACCGATGCTTCCTCCCTGAGAATTTCAGCCTCCCGCTGAGTGTGGACTTGGAGCC TTACTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAACGGCATGCGA TCCTGCAGGAGCGTGCCCACACTGCGCGGGGAAGGCGGTGGTGGTCCACCCTGCGGTCTGGACTATGAGG CCTATAACAGTTCCAGCAATACCACCTGTGTCAACTGGAACCAATACTATACCAACTGCTCTGCAGGCGA GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC ATCACACTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAACTTCATCT ACTTCATTCTTCTCATCATCTTCTCTGAGACCAAGCAACGGGAGAGTCAGCTGATGCGGGAGCAGCGTGT ACGATTCCTGTCCAATGCTAGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTTCTC AAGTACCTGGTGTACATCCTCCGCAAAGCAGCCCGCAGGCTGGCCCAGGTCTCTAGGGCTGTAGGCGTGC GGGCTGGGTTGCTCAGCAGCCCAGTGGTCCGTGGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCTCTCG TTCACACCGTCGTCTGTCTGTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTG GGTAATGGGACGCTCAGAGTTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCCCGCT GGCTCATGCTGCCACCACCCTCTACCCCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACA CAGCTTCTACCATGCTGACTGCCACTTGGAGCCAGTCCGTTGCCAGGCGCCCCCTCCCAGGTCCCCATCG GAGGCATCTGGCAGGACTGTGGGTAGTGGGAAGGTATACCCCACTGTGCATACCAGCCCTCCACCAGAGA TGCTGAAGGATAAGGCACTAGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACAT CCCACCTGGGCCCTTCAGCTCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCC TGCAAAATCTCCAGCCCTTGCTCCAAGGCAGACAGTGGAGCCTGTGGGCCGGACAGTTGTCCCTACTGTG CCCGGACAGGAGCAGGGGAGCCAGAGTCCGCTGACCATGAAATGCCTGACTCAGACAGTGAGGCTGTGTA TGAGTTCACACAGGACGCTCAGCACAGCGACCTCCGGGATCCCCACAGACGGCGACGGCCGAGCCTGGGC CCAGATGCAGAGCCTAGTTCTGTGCTGGCCTTCTGGAGGCTGATCTGCGACACATTCCGGAAGATCGTAG ATAGCAAATACTTTGGCCGGGGAATCATGATCGCTATCCTGGTCAATACCCTCAGCATGGGCATCGAGTA CCACGAGCAGCCCGAGGAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCC TTGGAGATGCTGCTGAAGCTGCTCGTCTACGGTCCCTTCGGCTACATTAAGAATCCCTACAACATCTTTG ATGGCGTCATTGTGGTCATCAGCGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCG GACCTTCCGCCTGATGCGGGTGCTGAAGCTAGTTCGCTTCCTGCCGGCACTGCAGCGGCAGCTCGTGGTG CTCATGAAGACCATGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCA TCCTGGGCATGCACCTTTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAA GAATTTTGACTCCCTGCTCTGGGCCATTGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAA GTCCTTTACAACGGCATGGCCTCCACGTCATCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTG GCAACTACGTGCTCTTTAACCTGCTTGTCGCCATTCTCGTGGAGGGTTTCCAGGCAGAGGGAGATGCCAC CAAGTCTGAGTCAGAGCCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGGAAGAAGCGCTTG GCCCTGGTGGCCTTGGGAGAACACTCGGAACTACGAAAGAGCCTTTTGCCACCTCTCATCATCCACACAG CTGCTACACCGATGTCACTGCCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCG CCGCACCAGTAGCAGTGGGTCCGCTGAGCCTGGAACTGCTCATCATGAGATGAAATCACCGCCAAGTGCC CGAAGCTCCCCGCACAGTCCCTGGAGCGCAGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCC TGGGCCGGGCCCCCAGCCTGAAGCGTAGGAGCCCAAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGG TCAGGAGAGCCAGGATGAGGAGGAGAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGATCATCGC CACAGGGGTTCCTTGGAACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACCCTGCAGGTGCCCGGGC TTCATCGAACAGCCAGCGGTCGGAGCTCTGCCTCTGAACACCAAGACTGTAATGGCAAGTCGGCTTCAGG GCGTTTGGCCCGCACCCTGCGGGCTGATGACCCCCCACTGGATGGGGATGATGGCGATGATGAGGGCAAC CTGAGCAAAGGGGAACGCTTACGAGCCTGGGTCCGAGCCCGGCTCCCTGCCTGTTGCCGAGAGCGAGATT CCTGGTCTGCCTATATCTTCCCTCCCCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAA GATGTTTGACCATGTGGTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAA ATTGACCCCCACAGCGCTGAACGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTCCTGG CTGAAATGACAGTGAAGGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTG GAACGTGCTGGACGGCTTGCTGGTGCTCATCTCTGTCATCGACATCCTGGTGTCCATGGTCTCTGACAGC GGCACCAAGATTCTCGGCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTACGTCCACTCAGGGTCATCA GCCGGGCCCAGGGGCTGAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGT GGTCATCTGCTGTGCCTTCTTCATCATTTTTGGAATTCTTGGGGTGCAGCTCTTCAAAGGGAAGTTCTTC GTGTGTCAGGGTGAGGACACCAGGAACATCACTAACAAGTCCGACTGTGCTGAGGCCAGTTACCGGTGGG TCCGGCACAAGTACAACTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGA TGGCTGGGTTGACATCATGTATGATGGACTGGATGCTGTGGGAGTGGACCAGCAGCCCATCATGAACCAC AACCCTTGGATGCTGCTCTACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTCGTCCTGAACATGTTTG TGGGCGTGGTGGTGGAGAACTTCCATAAGTGCAGGCAGCACCAGGAGGAGGAGGAGGCGCGGCGGCGGGA GGAGAAGCGACTAAAGAGGCTGGAGAAAAAGAGAAGGAATCTAATGTTGGACGATGTAATTGCTTCCGGC AGCTCAGCCAGCGCTGCGTCAGAAGCCCAGTGCAAACCCTACTACTCTGACTACTCGCGCTTCCGGCTCC TCGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGGTGTCATCGGGCTGAATGTGGT CACGATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCTCTGAAGATCTGCAACTACATC TTTACCGTCATCTTTGTCTTGGAGTCAGTATTCAAACTTGTGGCCTTCGGCTTCCGCCGGTTCTTCCAGG ACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCATGGGCATCACGCTGGAAGAGATTGA GGTCAATGCTTCACTGCCCATCAACCCCACCATCATCCGTATCATGAGGGTGCTCCGCATTGCTCGAGTT CTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACACGGTGATGCAGGCCCTGCCCCAGG TGGGGAACCTGGGACTTCTCTTCATGCTATTATTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTGG AGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGCCGGCATGCCACCTTTAGGAACTTTGGT ATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGAATGGTATTATGAAGGACACCCTCC GGGACTGTGACCAGGAGTCCACCTGCTACAACACCGTCATCTCACCCATCTACTTCGTGTCCTTCGTGCT GACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCCGTGCTGATGAAGCACCTGGAAGAGAGCAACAAA GAGGCCAAGGAGGAGGCGGAGTTGGAGGCGGAGCTGGAGCTAGAGATGAAGACACTCAGCCCGCAGCCCC ACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCTGGGGTGGAAGGTGTCAATAGCCCTGACAGCCCTAAGCC TGGGGCTCCACACACCACGGCCCACATTGGAGCAGCCTCTTCAGGCTTCTCCCTTGAGCACCCCACGATG GTACCTCACACTGAGGAGGGGCCAGTCCCCCTAGGACCAGACCTGCTGACTGTGAGGAAGTCTGGTGTCA GCCGGACACACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAGCACTGCCGAGAGATCCCTAGG ACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCTGTTCACTCCCAACCAGCAGAC ACCAGCTGCATCCTACAGCTTCCCAAAGATGCACACTATCTGCTCCAGCCTCATGGGGCTCCCACCTGGG GCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAGGCCTCTCAGGCGCCAGGCAGC AATAAGGACTGACTCCCTGGACGTGCAGGGCCTGGGTAGCCGGGAAGACCTGTTGTCAGAGGTGAGTGGG CCCTCCTGCCCTCTGACCCGCTCCTCATCCTTCTGGGGCGGGTCGAGCATCCAGGTGCAGCAGCGCTCCG GCAGCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCCAGGCCTGGAACCCAGCTGGGC CAAGGACCCTCAAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTGAGCTGGATTTCAGGAGACCTC CTGCCCAGCAGTCAGGAAGAACCCCTGTCCCCACGGGACTTGAAAAAATGCTACAGTGTAGAGGCCCAGA GCTGCCGGCGCAGGCCTGGGTCCTGGCTAGACGAACAGAGGAGACACTCCATCGCTGTCAGCTGCCTGGA CAGCGGCTCCCAGCCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGCCAACCTCTTGGGGGCCCTGGG AGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCCCGGAGAGCCAGGGCCCTCGGC CCCCATGCAGTCCTGGCGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGACTCGAAGGATCCCTCGGCCTC CAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGATGCGCTGAGTCTCTCTGGTTTGTCT TCTGACCCAACAGACCTGGATCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212118 representing BC057399 Red=Cloning site Green=Tags(s) MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR SCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV ITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELL KYLVYILRKAARRLAQVSRAVGVRAGLLSSPVVRGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHL GNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPS EASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSS CKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLG PDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFA LEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVV LMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNK VLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRL ALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSA RSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHR HRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGN LSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPK IDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDS GTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFF VCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNH NPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASG SSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYI FTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARV LKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFG MAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNK EAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTM VPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPAD TSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSG PSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDL LPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPG SRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLS SDPTDLDP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057399
ORF Size	6744 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057399, AAH57399
RefSeq Size	7527 bp
RefSeq ORF	6746 bp
Locus ID	12291

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