Pgbd5 (NM_171824) Mouse Tagged ORF Clone

CAT#: MG214261

  • TrueORF®

Pgbd5 (tGFP-tagged) - Mouse piggyBac transposable element derived 5 (Pgbd5), (10ug)

ORF Plasmid: DDK tGFP



  "NM_171824" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,470.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2900019M05Rik; AI854313
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG214261 representing NM_171824
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACCCAGCGCTAGCGCGGTGGACTTCTTCCAGCTCTTCGTCCCGGACAACGTGCTCAAGAACATGG
TGGTACAGACCAACATGTATGCCAGGAAGTTCCAGGAGCGGTTTGGCAGTGACGGTGCCTGGGTGGAGGT
GACGTTGGCAGAGATGAAGGCCTTCCTGGGCTATGTGATCTCCACCAGCGTCTCGCACTGTGAGTCGGTA
CTCAGCATTTGGAGCGGAGGCTTCTATAGCAACCGGAGCCTTGCCCTCGTCATGAGCCAGGCCCGCTTCG
AGAAAATCCTCAAGTACTTCCATGTTGTGGCCTTCCGATCCAGCCAGACCACGCATGGGCTCTACAAGGT
CCAGCCCTTCCTCGACTCCCTGCAGAGTGGCTTTGATGCTGCTTTCAGGCCGTCTCAGACCCAGGTGCTA
CATGAACCCCTGATTGACGAGGACCCTGTGTTCATTGCCACGTGCACGGAGCGAGAGCTGCGGAAGAGGA
AAAAGCGGAAATTCAGCCTGTGGGTCCGCCAGTGCTCTTCAACCGGGTTCATCATCCAGATCTACGTCCA
CCTAAAGGAAGGTGGTGGCCCAGATGGCCTGGACGCTCTGAAGAACAAGCCACAGCTGCACAGCATGGTG
GCCCGGAGCCTGTGCCGGAACGCGGCCGGAAAGAACTATATCATCTTTACAGGGCCCAGCATCACCAGCC
TCAATCTGTTCGAAGAATTTGAGAAGCAAGGGATCTACTGCTGTGGCCTCCTCAGCTCTAGGAAGAGTGA
CTGCACAGGTCTCCCTCCATCCATGTTGACCAACCCTGCCACCCCACTCGCCCGGGGCCAGCACCAGATC
AGGACAAAGGGCAACATGTCACTGATCTGCTGGTACAACAAGGGGCACTTCCGCTTCCTGACCAACGCCT
ACTCCCCTGTGCAGAAAGGCGTCATCATCAAGAGGAGGAGTGGGGAAATCCCCTGCCCTCTGGCCGTGGA
GGCCTTTGCGGCTCACCTCAGCTACATCTGCAGATATGACGATAAGTACAGCAAGTATTTCATCTCTCAC
AAGCCAAACAAGACGTGGCAGCAAGTGTTCTGGTTCGCCATCAGCATCGCCGTCAACAACGCCTACATCC
TGTACAAAATGTCAGACGCCTACCACGTGAAGAAGTACAGCCGGGCACAGTTCGGAGAGAGACTTGTCAG
GGAGTTGCTGGGCTTGGAGGACTCATCGCCAGCCCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG214261 representing NM_171824
Red=Cloning site Green=Tags(s)

MPPSASAVDFFQLFVPDNVLKNMVVQTNMYARKFQERFGSDGAWVEVTLAEMKAFLGYVISTSVSHCESV
LSIWSGGFYSNRSLALVMSQARFEKILKYFHVVAFRSSQTTHGLYKVQPFLDSLQSGFDAAFRPSQTQVL
HEPLIDEDPVFIATCTERELRKRKKRKFSLWVRQCSSTGFIIQIYVHLKEGGGPDGLDALKNKPQLHSMV
ARSLCRNAAGKNYIIFTGPSITSLNLFEEFEKQGIYCCGLLSSRKSDCTGLPPSMLTNPATPLARGQHQI
RTKGNMSLICWYNKGHFRFLTNAYSPVQKGVIIKRRSGEIPCPLAVEAFAAHLSYICRYDDKYSKYFISH
KPNKTWQQVFWFAISIAVNNAYILYKMSDAYHVKKYSRAQFGERLVRELLGLEDSSPAH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_171824
ORF Size 1227 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_171824.2, NP_741958.1
RefSeq Size 2854 bp
RefSeq ORF 1230 bp
Locus ID 209966
UniProt ID D3YZI9
Gene Summary The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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