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Nrde2 (NM_183155) Mouse Tagged ORF Clone
CAT＃: MG214512
- TrueORF®
Nrde2 (tGFP-tagged) - Mouse cDNA sequence BC002230 (BC002230), (10ug)
ORF Plasmid: DDK
"NM_183155" in other vectors (4)
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CNY 12,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6720454P05Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG214512 representing NM_183155
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGTTCCCGGCTTTTGCGGACGTAAGTGAGGCCTCCGGTGATGGGGCATTCAGGAAAGAGTTAG ATTGGCTGAGCAACCCAAGCTTTCGTGTTGGAAATCTAACTTCTCTGAGCCGACAAACTGAAGAGGTCAC AGCCCTTGCTTCTGAAGGGTCACCACCGCCAAGGAGTCCTCTGAAGTCAGAGCTCTCAGGTGAGAGCAAC ACTAGCGAGAAGCTCGCACAGACAAGCAGGAAGAAGAAGAAGGAGAAAAAGAAGAGACGGAAGCATCAGC ACCACAGGAAAACCAAGAGGAGGCACGAGCAGTTGAGCAGCAGCGGCTCTGAGTCAGACACAGAGGCTGG GAAGGACAGGGCTTCCCGGAGCATCAGGGACGACCAGAAGGAAGCCGAGAAACCCTGTCAGGGAAGCAAT GCGGCTGCTGCTGTTGCTGCTGCTGCTGGACATCGGTCCATTTGGCTGGAAGATATTCACGATCTGACCG ACGTCTTCAGGACTGATAAGAAGCCAGATCCTGCCAACTGGGAGTATAAATCTCTGTATCGTGGGGATAT AGCAAGGTACAAGAGGAAGGGAGACTCCTGCCTGGGTATCAATCCTAAGAAACAGTGTATATCTTGGGAA GGGGCTTCTGCAGCAAAGAAGCACTCACACAGGCACCTGGAGCGCTATTTTACCAAGAAGAATGTGGGGT TAATGAGAACAGAGGGCATTGCTGTGTGCAGTAACCCCGAGCCTGCCTCCTCCGAGCCCGTCACATTCAT CCCAGTGAAGGACTCTGCTGAGGCAGCTACTCCTGTTACATCATGGCTGAATCCTCTGGGGATTTATGAT CAGTCTACCACACAGTGGTTACAAGGACAGGGTCCAGCAGAGCAAGAGTCAAAGCAGCCGGACTCGCAAC AGGACAGGGAGAATGCTGCTCTCAAGGCCAGAGTGGAAGAGTTTAACAGAAGGGTTCGGGAGAATCCTTG GGACACGCAACTATGGATGGCGTTTGTGGCTTTCCAGGACGAGGTCATGAGGAGTCCTGGCATCTATGCC CTTGGGGAAGGAGAACAGGAGAAGCACAGGAAGTCCCTGAAGCTCCTCCTGGAGAAGAAGCTGGCTGTGC TGGAGCGGGCCATCGAGAGCAACCCAGGCAGCGTGGAGCTGAAGCTTGCCAAGCTGCAGCTGTGCTCAGA GTTCTGGGAGCCCAGTGCGCTGGCCAAGGAGTGGCAGAAGCTCCTCTTCCTGCACCCCAACAACACAAGC CTGTGGCAGCGCTATCTCTCCTTTTGCCAGAGCCAGTTTGGTACCTTTTCTGTGTCCAAACTCCACAGTC TGTATGGCAAGTGCTTGAGTACCTTGTCTGCTGTTAAAGATGGCAGCATGCTATCCCACCCTGTGTTGCC GGGCACCGAGGAGGCCATGTTTGGGCTCTTTCTTCAGCAGTGCCACTTTCTACGGCAGGCTGGCCACTCA GAGAAGGTCATCTCTTTGTTCCAGGCCATGGTTGACTTCACCTTCTTCAAGCCTGACAGTGTGAAAGAGC TGCCTACTAAAGTACAGGTGGAATTCTTTGAGCCCTTCTGGGACAGCGGAGAACCACGCGTTGGGGAGAA GGGAGCCCGAGGCTGGCGGGCGTGGATGCACCAACAGGAGCGAGGTGGCTGGGTGCTCATCACCCCAGAT GAGGATGACGAGGAGCCCGAAGAGGAGGACCAGGAGATAAAAGACAAGACCCTGCCCCGGTGGCAGATCT GGCTTGCTGTTGAGCGTTCTCGAGACCAGAGGCACTGGCGTCCCTGGCGCCCTGATAAGACCAAGAAGCA AACGGAGGAAGACTGTGAGGACCCTGAGAGACAGGTGCTGTTTGATGACATTGGGCAATCTTTGATCAGA CTTTCCAGCCCAGACCTTCAGTTCCAGCTCATCCAGGCCTTTCTGCAGTTCCTGGGCGTGCCTTCCGGCT TCCTGCCCCCAGCCTCCTGCCTTTACCTGGCCATGGACGAGAGCAGCATCTTTGAGAGTGAACTTTATGA TGAAAAGCCCCTGACTTACTTCAACCCTTCCTTTTCGGGCATTAGCTGTGTCGGCTCCATGGAACAGTTG GGTCGCCCTCGCTGGACCAAAGGTCACAACCGAGAGGGCGAGGAGTTTGTCCGCAATGTCTTCCACCTGG TGCTGCCTTTGCTCGCAGGCAAGCAGAAGTCCCAGCTCAGCCTCTCCTGGTTACGGTACGAGATTGCAAA GGTCATTTGGTGTCTACACACTAAAAAGAAGCGATTGAAGTCTCAGGGAAAGAGCTGCAAAAAACTAGCC AAGAATCTCCTCAAGGAGCCAGAAAACCGCAACAACTTCTGCCTCTGGAAGCAGTATGCACACCTGGAGT GGTTGCTCGGTAACACAGAGGATGCCAGAAAAGTGTTCGACACAGCACTCAGCATGGCAGGCAGCAGCGA GCTGAAGGACCGTGAACTCTGCGAGCTCAGTCTGCTCTATGCCGAGCTGGAGATGGAACTGTCGCCGGAC TCGAGAGGAGCCACCACAGGCCGAGCGGTCCACATATTAACACGGCTGACGGAGAGCAGTCCCTACGGGC CCTACACGGGGCAGGTCTCGTCCACCCAGGTGCTGAAAGCTCGGAAGGCTTACGAGCTCGCGCTGCAGGA CTGCCTGGGCCAGAGCTGTGCCTCCAGCCCAGCTCCTGCAGAGGCCTTGGACTGCCTGGGTAGCTTGGTC CGATGCTTTATGCTCTTCCAGTATTTGACTGTGGGCATTGATGCTGCTGTGCAGATATACGGAAGGGTGT TTGCCAAGCTGAAGGGCTCTGCTCGCTTAGAAGACCCAGGGCCAGAGGACAGCACCAGCTCCCAGAGTTT GACCAATGTGCTCGAGGCTGTCAGCATGATGCACACAAGCCTGCTGCGATTCCACATGAACGTTTGTGTT TACCCTCTGGCTCCACTCCGAGAGACCCTCTCCGATGCTTTAAAGCTGTATCCGGGCAACCAGGTTCTTT GGAGGGCTTATGTACAGATTCAGAATAAGTCCCACAGTGCTAACAAGACCAGAAGGTTCTTTGACACAGT TACTAGGTCTGCCAAACATTTGGAGCCTTGGCTATTTGCAATCGAAGCTGAGAAGCTGAGGAAAAAGCTA GTGGAATCTGTTCAGAGGGTAGGAGGCAGAGAGGTCCATGCAACAATCCCCGAGACTGGCCTTACGCATC GCATCAGAGCTTTGTTTGAAAATGCGATTCGGAGCGACAAGGGCAACCAGTGTCCCCTACTGTGGAGGAT GTATTTGAATTTTTTGGTTTCCTTAGGAAACAAAGAAAGAAGCAAAGGCGTGTTCTACAAGGCCCTCCAG AGCTGTCCTTGGGCAAAGGTGCTATACATGGACGCCATGGAGTATTTCCCAGACGAGCTGCAGGAGATCC TGGACGTGATGACCGAGAAGGAGCTCCGTGTGCGCCTGCCCCTTGAGGAGCTGGAGCTGCTGCTGGAGGA T AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214512 representing NM_183155
Red=Cloning site Green=Tags(s) MALFPAFADVSEASGDGAFRKELDWLSNPSFRVGNLTSLSRQTEEVTALASEGSPPPRSPLKSELSGESN TSEKLAQTSRKKKKEKKKRRKHQHHRKTKRRHEQLSSSGSESDTEAGKDRASRSIRDDQKEAEKPCQGSN AAAAVAAAAGHRSIWLEDIHDLTDVFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWE GASAAKKHSHRHLERYFTKKNVGLMRTEGIAVCSNPEPASSEPVTFIPVKDSAEAATPVTSWLNPLGIYD QSTTQWLQGQGPAEQESKQPDSQQDRENAALKARVEEFNRRVRENPWDTQLWMAFVAFQDEVMRSPGIYA LGEGEQEKHRKSLKLLLEKKLAVLERAIESNPGSVELKLAKLQLCSEFWEPSALAKEWQKLLFLHPNNTS LWQRYLSFCQSQFGTFSVSKLHSLYGKCLSTLSAVKDGSMLSHPVLPGTEEAMFGLFLQQCHFLRQAGHS EKVISLFQAMVDFTFFKPDSVKELPTKVQVEFFEPFWDSGEPRVGEKGARGWRAWMHQQERGGWVLITPD EDDEEPEEEDQEIKDKTLPRWQIWLAVERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIR LSSPDLQFQLIQAFLQFLGVPSGFLPPASCLYLAMDESSIFESELYDEKPLTYFNPSFSGISCVGSMEQL GRPRWTKGHNREGEEFVRNVFHLVLPLLAGKQKSQLSLSWLRYEIAKVIWCLHTKKKRLKSQGKSCKKLA KNLLKEPENRNNFCLWKQYAHLEWLLGNTEDARKVFDTALSMAGSSELKDRELCELSLLYAELEMELSPD SRGATTGRAVHILTRLTESSPYGPYTGQVSSTQVLKARKAYELALQDCLGQSCASSPAPAEALDCLGSLV RCFMLFQYLTVGIDAAVQIYGRVFAKLKGSARLEDPGPEDSTSSQSLTNVLEAVSMMHTSLLRFHMNVCV YPLAPLRETLSDALKLYPGNQVLWRAYVQIQNKSHSANKTRRFFDTVTRSAKHLEPWLFAIEAEKLRKKL VESVQRVGGREVHATIPETGLTHRIRALFENAIRSDKGNQCPLLWRMYLNFLVSLGNKERSKGVFYKALQ SCPWAKVLYMDAMEYFPDELQEILDVMTEKELRVRLPLEELELLLED SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183155 |
| ORF Size | 3501 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183155.3, NP_898978.2 |
| RefSeq Size | 3708 bp |
| RefSeq ORF | 3504 bp |
| Locus ID | 217827 |
| Gene Summary | Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation. Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation. Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome. By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223803 | Nrde2 (untagged) - Mouse cDNA sequence BC002230 (BC002230), (10ug) |
CNY 15,010.00 |
|
| MR214512 | Nrde2 (Myc-DDK-tagged) - Mouse cDNA sequence BC002230 (BC002230) |
CNY 11,690.00 |
|
| MR214512L3 | Lenti ORF clone of Nrde2 (Myc-DDK-tagged) - Mouse cDNA sequence BC002230 (BC002230) |
CNY 13,590.00 |
|
| MR214512L4 | Lenti ORF clone of Nrde2 (mGFP-tagged) - Mouse cDNA sequence BC002230 (BC002230) |
CNY 13,590.00 |
