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Cyp4x1 (NM_001003947) Mouse Tagged ORF Clone
CAT＃: MG215201
- TrueORF®
Cyp4x1 (tGFP-tagged) - Mouse cytochrome P450 family 4 subfamily x polypeptide 1 (Cyp4x1), (10ug)
ORF Plasmid: DDK
"NM_001003947" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A230025G20; Cyp4a28-ps; CYPIVX1; CYP_a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215201 representing NM_001003947
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCTCCTGGCTGGAGACTCGTTGGGCGCGGCCCTTGCACCTGGCTTTGGTGTTCTGCCTGGCGC TGGTGCTGATGCAGGCCATGAAGCTCTACCTGCGGAGACAGCGACTGCTGCGCGACCTGAGCCCCTTCCC GGGGCCTCCCGCCCACTGGCTCCTGGGACACCAGAAGTTTCTTCAGGAGGATAATATGGAGACGCTTGAT GAGATTGTCAAAAAGCACCCTTGTGCCTTCCCCTGCTGGGTAGGGCCCTTCCAGGCATTTTTCTACATCT ATGACCCAGACTATGCGAAGATATTTCTGAGCAGAACAGACCCAAAGATGCAGTATCTGCACCAGCTCCT GACTCCATGCATTGGACGAGGACTCCTGAATTTAGATGGGCCCAGGTGGTTCCAACACCGCTGCCTCCTA ACTCCTGCATTCCATCAGGACATCCTAAAACCATGTGTGGACACGATGGCCCACTCTGTGAAAGTGATGC TGGATAAATGGGAGAAGATGTGGACCACTCAGGAAACAACCATCGAGGTTTTTGAACACATCAACTTGAT GACCCTGGACATAATAATGAAATGTGCTTTTGGCCAGGAGACCAACTGCCAGATAAACGGCACCTATGAG TCTTACGTGAAGGCAACATTTGAACTTGGTGAAATCATATCTTCTCGCTTGTACAATTTCTGGCATCATC ATGACATAATTTTCAAACTCAGTCCTAAGGGCCACTGCTTCCAGGAGTTAGGCAAAGTGATACATCAATA CACAGAAAAGATAATCCAGGACAGAAAGAAAATCCTCAAGAATCAAGTAAAGCAGGATGACACTCAGACG TCTCAAATTTTTCTGGATATTGTTCTTTCTGCACAGGCTGAAGATGAACGAGCCTTCTCAGATGCTGACC TTCGGGCTGAGGTGAACACCTTCATGTGGGCAGGACATGATGCCTCTGCAGCTAGCATCTCCTGGCTCCT TTACTGCCTGGCTCTAAATCCCGAGCATCAGGACAGATGCCGGACAGAGATCAGGAGCATCCTGGGAGAT GGGTCTTCCATCACCTGGGAACAGCTGGATGAGATGTCATACACCACAATGTGCATCAAGGAGACACTCC GCTTGATTCCTCCTGTCCCATCCATCTCCAGGGAGCTCAGCAAACCCCTTACCCTCCCAGATGGACACTC ACTGCCTGCAGGTATGACTGTGGTTCTTAGTATTTGGGGTCTCCACCACAACCCTGCTGTCTGGAACGAC CCAAAGGTCTTTGACCCCTTGAGATTCACTAAGGAGAATTCTGATCAGAGACACCCCTGTGCCTTCCTAC CATTCTCCAGTGGTCCAAGGAACTGCATCGGGCAGCAGTTTGCCATGCTGGAGCTAAAGGTGGCCATTGC CTTGATTCTGCTCCACTTTCAAGTAGCTCCAGACCTCACCAGGCCTCCTGCCTTCTCCAGCCACACTGTC CTCAGACCCAAGCATGGAATCTATTTGCACCTGAAGAAACTCCTTGAGTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215201 representing NM_001003947
Red=Cloning site Green=Tags(s) MEASWLETRWARPLHLALVFCLALVLMQAMKLYLRRQRLLRDLSPFPGPPAHWLLGHQKFLQEDNMETLD EIVKKHPCAFPCWVGPFQAFFYIYDPDYAKIFLSRTDPKMQYLHQLLTPCIGRGLLNLDGPRWFQHRCLL TPAFHQDILKPCVDTMAHSVKVMLDKWEKMWTTQETTIEVFEHINLMTLDIIMKCAFGQETNCQINGTYE SYVKATFELGEIISSRLYNFWHHHDIIFKLSPKGHCFQELGKVIHQYTEKIIQDRKKILKNQVKQDDTQT SQIFLDIVLSAQAEDERAFSDADLRAEVNTFMWAGHDASAASISWLLYCLALNPEHQDRCRTEIRSILGD GSSITWEQLDEMSYTTMCIKETLRLIPPVPSISRELSKPLTLPDGHSLPAGMTVVLSIWGLHHNPAVWND PKVFDPLRFTKENSDQRHPCAFLPFSSGPRNCIGQQFAMLELKVAIALILLHFQVAPDLTRPPAFSSHTV LRPKHGIYLHLKKLLEC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003947 |
| ORF Size | 1521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001003947.2 |
| RefSeq Size | 1524 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 81906 |
| UniProt ID | Q6A152 |
| Gene Summary | A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217187 | Cyp4x1 (untagged) - Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1), (10ug) |
CNY 6,560.00 |
|
| MR215201 | Cyp4x1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1) |
CNY 3,776.00 |
|
| MR215201L3 | Lenti ORF clone of Cyp4x1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1) |
CNY 6,370.00 |
|
| MR215201L4 | Lenti ORF clone of Cyp4x1 (mGFP-tagged) - Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1) |
CNY 6,370.00 |
