Apopt1 (NM_001163388) Mouse Tagged ORF Clone
CAT#: MG215274
- TrueORF®
Apopt1 (tGFP-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001163388" in other vectors (4)
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CNY 2,090.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1700081D05Rik; 2810002N01Rik; Apop-1; Apop1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215274 representing NM_001163388
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTGCGGCCCGGAAGCAGAGCGCTTCGCCGGCTTCTCTGCCGGTCCTTCTCCGGCGGCGGCG GCGTTAGGTTGGCCCGTGAGCGCCCTACGGATCACAGGGATGCGGCGTCCAGCCGGGTCTCAAGATTCTG CCCTCCAAGACAGTCTTGCCATGATTGGATAGGCCCCCCAGACAAATGCTCAAACCTTCGTCCCGTTCAT TTTCACATCCCTGAAAACGAGTCTCCCTTGGAGCAAAGACTTAGAGAATTGAGACAAGAAACGCAAGAAT GGAATCAACAGTTCTGGGCCAAGCAGAACTTGTCCTTCAATAAGGAAAAAGAAGAATTCATTTACTCAAG ACTACAAGCTAAGGGCGCAGGCTTGCGGACTGAGTCAGGCCAAAGAGCAACGCTGGATGCAGAGGAGATG GCCGACTTCTATAAGGACTTTCTGAGTAAGAATTTCCAGAAGCACATGCGCTATAACCGGGCTGGGCCTG GGAGCCAGGATGTTGGATGTGTTGGCAAGTGCTCTTTTGCCGAGCTGCATCCTTACCTCACAGTGTCCGC CTTACAAGGTTCCTGTCCTGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215274 representing NM_001163388
Red=Cloning site Green=Tags(s) MAALRPGSRALRRLLCRSFSGGGGVRLARERPTDHRDAASSRVSRFCPPRQSCHDWIGPPDKCSNLRPVH FHIPENESPLEQRLRELRQETQEWNQQFWAKQNLSFNKEKEEFIYSRLQAKGAGLRTESGQRATLDAEEM ADFYKDFLSKNFQKHMRYNRAGPGSQDVGCVGKCSFAELHPYLTVSALQGSCPA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001163388 |
ORF Size | 582 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163388.1, NP_001156860.1 |
RefSeq Size | 684 bp |
RefSeq ORF | 585 bp |
Locus ID | 68020 |
UniProt ID | Q9CQW7 |
Gene Summary | Plays a role in the regulation of apoptosis. Mediates mitochondria-induced cell death in vascular smooth muscle cells through the release of cytochrome c from mitochondria, followed by the activation of the caspase cascade.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210674 | Apopt1 (untagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR215274 | Apopt1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 1 |
CNY 1,900.00 |
|
MR215274L3 | Lenti ORF clone of Apopt1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 1 |
CNY 3,800.00 |
|
MR215274L4 | Lenti ORF clone of Apopt1 (mGFP-tagged) - Mouse RIKEN cDNA 2810002N01 gene (2810002N01Rik), transcript variant 1 |
CNY 3,800.00 |