United States
Germany
Japan
United Kingdom
China
Tpo (NM_009417) Mouse Tagged ORF Clone
CAT＃: MG215425
- TrueORF®
Tpo (tGFP-tagged) - Mouse thyroid peroxidase (Tpo), (10ug)
ORF Plasmid: DDK
"NM_009417" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215425 representing NM_009417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAACACTTGGAGCTATGGCAATAATGCTGGTGGTTATGGGAACTGTAATTTTCCTCTCTTTTATCC TGAGAAGCAGAGACATCTTGTGTGGGAAGACCATGAAGTCCCATGTTATCAGTGCTGTGGAAACGAGCCA GCTCATGGTGGACCATGCAGTCTACAACACCATGAAAAGAAACCTCAAGAAAAGGGAAGTCCTTTCTCCA GCCCAGCTTCTCTCTTTCTTTAAGCTGCCCGAGTCCACCAGTGGGGCTATTTCCCGAGCAGCAGAGATTA TGGAAACATCAATACAAGTCATGAAACGTGAACAGTCACAGTTCTCCACGGATGCCTTATCAGCAGACAT TCTGGGCACAATTGCCAACCTGTCAGGATGCTTGCCTTTCATGCTGCCACCAAGATGTCCTGACACCTGC CTGGCAAATAAGTACCGGCCCATCACAGGGGCGTGCAACAATAGAGATCACCCCAGATGGGGAGCCTCCA ACACAGCCCTAGCAAGATGGCTGCCTCCTGTCTATGAAGATGGCTTCAGTCAGCCCAAAGGCTGGAACCC TAATTTCTTATACCACGGCTTCCCACTGCCCCCGGTACGGGAAGTGACAAGGCACCTCATTCAAGTTTCC AATGAGGCTGTGACCGAAGATGACCAGTACTCTGATTTTCTGCCGGTGTGGGGACAGTACATCGATCATG ACATTGCTCTCACACCACAGAGCACTAGCACAGCAGCCTTCTGGGGAGGTGTCGACTGCCAGCTGACCTG TGAGAACCAAAATCCTTGCTTCCCCATACAGCTTCCCTCAAACTCCTCAGGGACCACTGCATGCCTGCCT TTCTACCGCTCCTCCGCCGCTTGTGGCACTGGGGACCAAGGTGCTCTCTTTGGCAACCTGTCTGCAGCCA ATCCGAGGCAGCAGATGAATGGCTTGACCTCCTTCCTTGATGCTTCCACTGTGTACGGCAGCTCCCCTGG CGTTGAGAAGCAGCTGCGCAACTGGAGCAGCTCGGCAGGACTGCTGCGTGTCAACACTCTCCACCTAGAT GCTGGCCGTGCCTACCTGCCCTTCGCAACAGCCGCCTGCGCTCCAGAGCCTGGTACCCCACGCACCAACC GCACGCCCTGCTTCCTGGCTGGAGACGGTCGCGCCAGTGAGGTCCCTGCCCTGGCAGCGGTACACACCTT GTGGCTGCGCGAGCACAACCGCCTGGCTTCGGCCTTCAAGGCCATTAACAAGCACTGGAGCGCCAACACT GCCTACCAGGAGGCGCGCAAGGTGGTAGGGGCACTGCACCAGATCATCACCATGAGGGATTATATCCCCA AAATCCTGGGTCCTGATGCCTTCAGGCAGTATGTGGGCCCCTATGAAGGCTACAACCCCACTGTGAACCC TACTGTGTCCAACATCTTCTCCACTGCTGCCTTTCGCTTTGGCCATGCCACAGTCCATCCACTGGTGAGA CGGCTAAACACTGACTTCCAGGAGCACACAGAGCTCCCCAGGTTGCAGCTGCGTGATGTCTTCTTCAGAC CCTGGAGGCTTATCCAGGAAGGTGGTTTGGATCCGATAGTGAGAGGCCTCCTGGCGAGAGCAGCCAAGCT GCAAGTCCAAGGGCAGCTGATGAATGAGGAGCTGACCGAGAGGCTCTTCGTGCTGTCTAACGTGGGCACC TTGGATCTGGCATCACTGAACTTGCAGAGGGGCCGGGATCATGGCTTACCAGACTACAATGAATGGAGAG AGTTCTGTGGCTTGTCACGCCTGGAGACACCAGCTGAGCTGAACAAGGCCATTGCCAACAGAAGCATGGT CAACAAGATAATGGACTTATACAAGCATGCTGACAACATTGACGTCTGGTTGGGAGGCTTGGCTGAAAAG TTCTTGCCGGGGGCCCGCACTGGTCCTCTGTTTGCATGTATCATTGGGAAGCAGATGAAGGCTCTGAGGG ATGGGGACAGGTTTTGGTGGGAGAACACCAATGTCTTCACAGACGCTCAGAGGCAGGAACTAGAAAAGCA TTCACTACCTCGGGTCATCTGTGACAACACTGGACTCACCAGAGTACCTGTGGATGCCTTCCGTATTGGA AAGTTCCCCCAAGACTTTGAATCCTGTGAGGACATCCCGAGCATGGACCTTGAACTATGGAGGGAGACCT TCCCACAAGATGACAAGTGTGTCTTCCCAGAGGAGGTGGACAATGGGAACTTTGTGCACTGTGAAGAGTC TGGGAAGCTGGTGCTTGTATACTCCTGTTTCCATGGATACAAGCTGCAGGGCCAGGAGCAGGTCACATGT ACCCAGAAGGGATGGGACTCCGAACCTCCTGTCTGTAAAGATGTTAATGAGTGTGCAGATCTGACACACC CACCTTGCCACCCCTCTGCACAGTGCAAGAACACCAAGGGAAGCTTCCAGTGTGTGTGCACAGACCCCTA TGTGCTAGGTGAGGATGAGAAGACCTGCATAGATTCTGGCAGGCTACCTCGGGCATCCTGGGTCTCCATT GCACTGGGTGCACTTCTCATTGGTGGTTTGGCCAGTCTCACCTGGATAGTAATTTGCAGGTGGACACATG CTGATAAGAAAGCCACATTGCCGATCACAGAGAGAGTGACCACCCAGTCAGGATGCAGAAAGAGTCAGGG GAGGGGGATTTCACCACACAAGGCCGCAGCTCAAGACACTGGACAGGAACCTGCAAGTGGATCCAGGGTC CTCCTGTGCGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215425 representing NM_009417
Red=Cloning site Green=Tags(s) MRTLGAMAIMLVVMGTVIFLSFILRSRDILCGKTMKSHVISAVETSQLMVDHAVYNTMKRNLKKREVLSP AQLLSFFKLPESTSGAISRAAEIMETSIQVMKREQSQFSTDALSADILGTIANLSGCLPFMLPPRCPDTC LANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPKGWNPNFLYHGFPLPPVREVTRHLIQVS NEAVTEDDQYSDFLPVWGQYIDHDIALTPQSTSTAAFWGGVDCQLTCENQNPCFPIQLPSNSSGTTACLP FYRSSAACGTGDQGALFGNLSAANPRQQMNGLTSFLDASTVYGSSPGVEKQLRNWSSSAGLLRVNTLHLD AGRAYLPFATAACAPEPGTPRTNRTPCFLAGDGRASEVPALAAVHTLWLREHNRLASAFKAINKHWSANT AYQEARKVVGALHQIITMRDYIPKILGPDAFRQYVGPYEGYNPTVNPTVSNIFSTAAFRFGHATVHPLVR RLNTDFQEHTELPRLQLRDVFFRPWRLIQEGGLDPIVRGLLARAAKLQVQGQLMNEELTERLFVLSNVGT LDLASLNLQRGRDHGLPDYNEWREFCGLSRLETPAELNKAIANRSMVNKIMDLYKHADNIDVWLGGLAEK FLPGARTGPLFACIIGKQMKALRDGDRFWWENTNVFTDAQRQELEKHSLPRVICDNTGLTRVPVDAFRIG KFPQDFESCEDIPSMDLELWRETFPQDDKCVFPEEVDNGNFVHCEESGKLVLVYSCFHGYKLQGQEQVTC TQKGWDSEPPVCKDVNECADLTHPPCHPSAQCKNTKGSFQCVCTDPYVLGEDEKTCIDSGRLPRASWVSI ALGALLIGGLASLTWIVICRWTHADKKATLPITERVTTQSGCRKSQGRGISPHKAAAQDTGQEPASGSRV LLCE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009417 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009417.3, NP_033443.1 |
| RefSeq Size | 3291 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 22018 |
| UniProt ID | P35419 |
| Gene Summary | This gene encodes a membrane-bound glycoprotein. The encoded enzyme plays a central role in thyroid gland function. The enzyme functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mice with homozygous missense mutations in this gene exhibit hypothyroid dwarfism and hearing impairment. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222583 | Tpo (untagged) - Mouse thyroid peroxidase (Tpo), (10ug) |
CNY 11,780.00 |
|
| MR215425 | Tpo (Myc-DDK-tagged) - Mouse thyroid peroxidase (Tpo) |
CNY 6,696.00 |
|
| MR215425L3 | Lenti ORF clone of Tpo (Myc-DDK-tagged) - Mouse thyroid peroxidase (Tpo) |
CNY 9,980.00 |
|
| MR215425L4 | Lenti ORF clone of Tpo (mGFP-tagged) - Mouse thyroid peroxidase (Tpo) |
CNY 9,096.00 |
