Parp4 (NM_001145978) Mouse Tagged ORF Clone
CAT#: MG215472
- TrueORF®
Parp4 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family member 4 (Parp4), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001145978" in other vectors (2)
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CNY 21,660.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Adprtl1; C030027K23Rik; E230037B21Rik; Gm743; p193; PARPL; PH5P; VAULT3; VPARP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215472 representing NM_001145978, codon optimized.
Due to the complexity of NM_001145978, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTGGGCATTTTCGCTAACTGCATCTTCTGTCTCAAGGTCAAGTACCTTCCCCGACAGCAGAAAA AAAAGCTCCAAACAGATATTAAGGAAAACGGGGGGAAGTTTTCCTTTCTTCTCAATCCTCAGTGTACCCA CGTCATTGTAGACTCTGCAGACGTCCTCAGTCGGTGCCACCTGAATAGCATCCAGAAGAATGACGTTCAG ATCGCTAACCCTGCATTCATCCAGGATAGCGTACGGCAGAGGAGACTGCTGGACGTTAGGAATTACGATC CCCTGTCCCCAGCACCTGCTGCTCCCCCGGCTGAACGGAGCCGCTCTGAAGTGCAGTCTGAGTACCTTCC CAGCGACAACACCCCAGAAAAGGAGAACACTGAAGTTACTGAGGTGTCTGCTGAGAACGTGGAGATCCCA CCTTTCCTCCAGGACTTCGAGGTTGTGAAATATAATATTCTCGAAAAGGTCGGGGGTCCCGAAACCGTGG TGGTGGAGCTTCAGAGTTCCCAGGACCCTGAAAGCTGCCCGTTTGTCATCACCGCCCATTTCCTGCTCGC CGACCAGAAAACTCGACGGGAGTCAACGGGAAAGCAGACCTCTGAAGGAGCCATCGAATACTACGAGTCA TATGTCGAGGATCTGAAGCGACAGGGGTTCCTGCTGCAGGAGCATTTCACAGCAGAAGCAACTCAGTTGG CCAGCGAAAAACTGCAGGCACTGCTGCTGGAGGAGGTGATCTCCAGCGGGGCATTGAGCCAAGAGGTTTC AGATCTTCTCGAGGTTATCTGGACCGAGGCCCTCGGACATTTGGAGAATACACTCCTTAAGCCTGTTAAT TCTATGAGTTTGAATGATGTGTCTAAGGCTGAGGGAATACTGCTCCTGGTTAAAACAGCACTCAAAAACG GCGATAGCCCTGGGCAGCTGCAGAAGACTATGGCTGAGTTCTATAGGCTCCTCCCGCACAGGCACCCAGC ATCTGAGGAGGTCAATCTCAGGCTGTTGGCTCAAAAAGAGGACCTCTGCCAGTTGGTAAGAGACATGGTC AATGTATGTGAAACCAACCTGAGTAAACCAAATCCCCCTAGCCTCGCCAAGTACCGCGCTTTGAGGTGTA AGATCGAGCATGTTGACCAGAATACCGAAGAGTTCAGCCGGGTCAGGAAGGAAGTGTTGCAGAATAACCG GAGCGAGCAGCCCGTGGACATCCTGCAAATATTCCGGGTGGGCAGGGTAAATGAGGCCACAGAGTTCCTG AGTAAGCTGGGAAATGTTCGCCTGCTGTTTCACGGATCCCCGGTAAGAAACATTCTCGGAATCCTCTCCA GAGGGCTGCTGCTGCCAAAGGTAGCCGAGGACAGGGGTGTTCAGAGAACCGACGTGGGCAACTTGGGGAG CGGGATCTACTTTTCTGATTCCCTGAGCACCAGCATTAAGTACGCCCATGCCGGTGAGACTGACGGCTCC CGGCTGCTCGTAGTGTGTGACGTTGCCCTGGGAAAGTGCGTGAATCTGTTTAAGAAAGATTTCTCCCTGA CAGAAGCCCCTCCAGGGTATGACTCAGTCCATGGAGTTTCCGAAACCACAAGTGTGCCCACCGACTTCCA AGACGATGAGTTCGTGGTATATAAGACTAATCAGGTCAAAATGAAGTACATCGTCAAATTCTGTACCCCT GGGGACCAGATTAAAGAATTTCACCCCCACGAGAATACAGAGGTGGAGGAGCAAAGAGCTGAACCTAGCA GTGTTCCAGAGGCCGGCGACTTTCAGCTGCCCGACATTAAGCCCTTTACAAATATAAAAGCGGGTTTGCA GGATGCCTCCGCTAACCCCGTCCCTCTCGACTCTGTACACATCAAGGGCAGGGTCATCGACTTTGTTGCA CAGGTGATCGTCTTTCAGACTTATACCAACCAGTCCCACGTGCCAATAGAGGCAAAATATATCTTCCCTC TGGATGACAAGGCAGCCGTGTGTGGGTTCGAAGCATTCATAAACGGAAAGCACATAGTTGGGGAAATCAA GGAAAAAGAAGAGGCCCGACAGGAGTACCGAGAGGCCGTCTCTCAGGGCCATGGTGCATACCTGATGGAC CAAGACACCCCTGACGTATTTACTGTTTCAGTCGGGAATCTGCCACCTCGGGCCAAAGTCCTGATTAAGA TCACGTACATCACGGAGCTTTCTATACAATCTCCCGTGGCCATCTTCTTCATTCCAGGAACAGTAGCACC CTGGCAGCAGGACAAAGCGTTGAACGAGAACTTGCAGGATACAGTTGAAACAATCCGCATTAAGGAAATC GGAGCTGAGCAAAGTTTCTCCCTGGCTATGAGCATTGAAATGCCGTACATGATCGAGTTCATCAGTAGTG ACACCCACGAACTGAGACAGAAAAGCACAGATTGTAAAGCAGTCGTCAGCACAGTGGAAGGCAGTAGTCT CGATTCTGGAGGGTTCTCCTTGCATATCGGCCTGCGGGACGCTTACCTGCCTCGCATGTGGGTGGAGAAA CACCCTGAGAAGGAATCTGAGGCCTGCATGCTCGTGTTTCAGCCAGAGCTGGCCGACGTCTTGCCAGACC TGCGGGGAAAGAATGAGGTCATCATCTGCCTGGACTGCAGCTCTTCCATGGAAGGAGTAACCTTTACCCA AGCTAAGCAGGTGGCACTGTATGCCCTCAGTCTCCTGGGGGAAGAACAGAAAGTCAATATTATGCAATTC GGGACCGGGTACAAGGAATTGTTCAGCTACCCCAAGTGTATCACAGACAGTAAGATGGCAACTGAGTTCA TTATGTCCGCCGCACCCTCAATGGGTAATACAGATTTTTGGAAGGTGCTGAGGTATCTGTCACTGCTGTA CCCCAGCGAAGGATTCCGCAATATTCTGCTCATCTCTGACGGCCACCTCCAGTCTGAAAGCCTCACCCTG CAGCTGGTGAAGAGGAACATTCAGCATACGCGAGTCTTCACTTGTGCAGTGGGGAGCACAGCAAACAGGC ACATCCTGCGCACCCTGTCTCAGTGCGGAGCCGGCGTGTTCGAGTATTTTAACAGCAAGAGCAAACACTC TTGGAAGAAGCAGATTGAGGCCCAAATGACAAGGATCAGATCTCCCTCATGTCATTCTGTCAGCGTAAAA TGGCAGCAGCTCTCCCGCGATGCTCCTGAACCCTTGCAGGCCCCTGCATGGGTTCCTAGCCTTTTCCATA ATGATCGCCTGTTGGTTTACGGGTTCATTCCTCATTGTACCCAGGCAACCCTGCAAGCCTTTATTCAGGA GAAAGAGTTTTGTACCATGGTTAGCACCACCGAACTGCAGAAGACCACTGGCACTATGATCCATAAATTG GCAGCACGGGCACTGATCAGGGATTATGAGGACGGGATCCTGCACGATGATGAGACGAACCATGAGATGA AGAAGAACATCATGAAATCTCTGATAATCGAACTTAGCAAGGAGAACAGTCTGATAACACAGTTTACTTC CTTCGTAGCAGTGGAAAAGCGAGATGTGAATGAAATTCCGTTCGCAAACGTCCCTAATATTAGTGAACTG GTCGCAAAGGAGGATGTGGACTTCTTGCCATACGTTTCTTGGCAGGAGAAGCAGCCAGAGGCCAGTATTT CCCAGACCGAAATTGATTCATCACGCCTCAAGCACAATAAACTGTCTGATGGACATGGTGTACTTCAGCC AGTCAGCGTGTCTAGCGAAGTGAACGAAAAACCTAGCCTTCTGCTTGCAGCTAAAAAGCGCAAGATTAAG ACTATTAAGAAATGCAGCCTTGATATCAGCGAAGATTTCGAGGACCGAACTGCTGTGGCCCAGTCCCCCG CAACTGCACAATCCTTGAATTTCCATCTTCCTCTGTCAGTGCGCCCTCAGCTCAAGGCCGTCGAGCAGCA GCTGCACGGCAACAGACTGGAACCCAAACAGCGCGGAGGATTTCGGAAACTCCTGATGGCCAAAAAGTGC AGGAACGTGCCCGACTCCCTTGTCTCCAGCGCCCCTGCTGTTACGGCCGAGTTCTCATATCTGTCCGCAT GCTCCTCATCTTCTGCTTTTCTTAGCCCACTGTGCGACATCCCTTCATCACTGCCCCCCCATCCCCTCGG TGGTACTCATCCCCCCCCTCCACTCCCACTGCCTGACGGGACTCACCTGCCATCTCCACTCTTCGGAAGC ACTCATCCTCCACCACCACTGTTCGGTGGCACTCTCATCCCACCTCCTTCTAGCCTCTTCGGCGGAACAC ACCTTCCACCACCTCCACCCCTGCCTGGGGGAACGCACATACCACCACCTCCACCCATTCCTGGAGGAAC TCTCATCCCTCCCTCTAGTTCCCTTTTCGGCGGGACACATCTGCCTCCTCCGCCTCTCCTGTCCGCAGGC ACACATATTCCACCACCTCCTTTGCTGTCAGCTGGCACGCACTTGCCTCCTCCCCCCCTCCTGCCAGCTG GCACCCACATACCTCCACCACCACCCATCACCGGGTCTACTCACCCACCTCCACCGTCATCTCTGTTCGG GGGCACACATCTGCCGCCCCCACCCCCCCTGCCTGGCGGTACTCACATTCCTCCGCCACCTCCTATACCT GGCGGAACATTGATCCCATCCCCCTCCTCACTGTTCGGCGGAACACACCTTCCCCCTCCCCCTCTTCTCC CTGCGGGAACCCATATCCCGCCGCCCCCTCCCATCACAGGATCTACCCACCCACCCCCCCCCTCCAGCCT GTTCGGGGGGACCCATCTTCCTCCGCCTCCTCCCGCAGGCACTCAGTTCTCTCTGAGCCCCATAGGCTTT ATTCCTCCTAAGCTGGGCCCCCCAAAGCTCAGCCATAGTCACAAGTTGGTCGGCGATACCAATATACATG ACAGCGAGCCACCCCTGTTGGGGTTTAAAGATCTCTGTAGCCGAGACATGGGCTTCTCTTGTGGAACAGC ATTTTCTGGGAGCTTCGCTTCTTCCAAAGACTTCGACCCAGGGAAGTTCAGCCAGGGACCAAACAACATC AGCTTTTCCCCTAAGGCCCCAGAAATGGGAGTGCTTCATCAGTCTCCGTTCTGCAGCCCGCCAAAGCCAC CTTCCGCACCTCCATTGGTGACAAATGTGCTGTGCTCAGAGGCACCTCAATCTTATTTCCTGAATCTTCA GAGCGCCGCTGTTCACCAGTCCCCCAACAACCGCGTGTCAGAGATCATAATGGAATCAGTGGAGAGTTCT TTGCCGTCAGACTATTCTAGCCGGGATGCTTCTAGCTACCTTGCTCTCGAGGGGGCCGAGGACTCTCTCC TGGGTGGGTCCTCCTTTGAAACAGACACCGACGAGGCCGCCGCTTTCATAGCGAATGATCTCCTCACAAG CATTGAAACCAGCTCTGATGAGGAGTGTGCTTTCTGCGATGAGGACCAGGAGTCCCCCGTACCATGGGCA AGCCTTTTCGCACTGCAGACGGAGAACGGGTTCTGGAAGCTTACACCTGAACTTGGTCTGATCCTGAATC TGAACGTGAACGCTCTGCTGACCAGCCTGGAAGAGAAGGGAATTAGGAGCCTTGGCACCAAGGGCCGCGA ACGGCTCCTCGACTTGATAGCTACTCTGCTGGTGCTGCAGTTCCTTTACACCAAGCTGGAGCAAGAGGGA ATGGTGGCAAAAAGTTTGATCAAAATGGATGATGCCTTTATCAGCAGGAACATACCCTGGGCATTCGAAA ATATCAAGAAGGCACGGGAGTGGGCTCGCAAGACAGAGGGCCAGTACCCCTCAATTTGTCAGCGCTTGGA GCTCGGAAAAGACTGGGAATCTGCGACCAAGCAGCTCTTGGGAATCCAGCCTCAGGCAAATACCTCTCTG CACCGCATCCTGTACTACAGCCAGGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215472 representing NM_001145978
Red=Cloning site Green=Tags(s) MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQ IANPAFIQDSVRQRRLLDVRNYDPLSPAPAAPPAERSRSEVQSEYLPSDNTPEKENTEVTEVSAENVEIP PFLQDFEVVKYNILEKVGGPETVVVELQSSQDPESCPFVITAHFLLADQKTRRESTGKQTSEGAIEYYES YVEDLKRQGFLLQEHFTAEATQLASEKLQALLLEEVISSGALSQEVSDLLEVIWTEALGHLENTLLKPVN SMSLNDVSKAEGILLLVKTALKNGDSPGQLQKTMAEFYRLLPHRHPASEEVNLRLLAQKEDLCQLVRDMV NVCETNLSKPNPPSLAKYRALRCKIEHVDQNTEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFL SKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGS RLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKFCTP GDQIKEFHPHENTEVEEQRAEPSSVPEAGDFQLPDIKPFTNIKAGLQDASANPVPLDSVHIKGRVIDFVA QVIVFQTYTNQSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEARQEYREAVSQGHGAYLMD QDTPDVFTVSVGNLPPRAKVLIKITYITELSIQSPVAIFFIPGTVAPWQQDKALNENLQDTVETIRIKEI GAEQSFSLAMSIEMPYMIEFISSDTHELRQKSTDCKAVVSTVEGSSLDSGGFSLHIGLRDAYLPRMWVEK HPEKESEACMLVFQPELADVLPDLRGKNEVIICLDCSSSMEGVTFTQAKQVALYALSLLGEEQKVNIMQF GTGYKELFSYPKCITDSKMATEFIMSAAPSMGNTDFWKVLRYLSLLYPSEGFRNILLISDGHLQSESLTL QLVKRNIQHTRVFTCAVGSTANRHILRTLSQCGAGVFEYFNSKSKHSWKKQIEAQMTRIRSPSCHSVSVK WQQLSRDAPEPLQAPAWVPSLFHNDRLLVYGFIPHCTQATLQAFIQEKEFCTMVSTTELQKTTGTMIHKL AARALIRDYEDGILHDDETNHEMKKNIMKSLIIELSKENSLITQFTSFVAVEKRDVNEIPFANVPNISEL VAKEDVDFLPYVSWQEKQPEASISQTEIDSSRLKHNKLSDGHGVLQPVSVSSEVNEKPSLLLAAKKRKIK TIKKCSLDISEDFEDRTAVAQSPATAQSLNFHLPLSVRPQLKAVEQQLHGNRLEPKQRGGFRKLLMAKKC RNVPDSLVSSAPAVTAEFSYLSACSSSSAFLSPLCDIPSSLPPHPLGGTHPPPPLPLPDGTHLPSPLFGS THPPPPLFGGTLIPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIPGGTLIPPSSSLFGGTHLPPPPLLSAG THIPPPPLLSAGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIP GGTLIPSPSSLFGGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPAGTQFSLSPIGF IPPKLGPPKLSHSHKLVGDTNIHDSEPPLLGFKDLCSRDMGFSCGTAFSGSFASSKDFDPGKFSQGPNNI SFSPKAPEMGVLHQSPFCSPPKPPSAPPLVTNVLCSEAPQSYFLNLQSAAVHQSPNNRVSEIIMESVESS LPSDYSSRDASSYLALEGAEDSLLGGSSFETDTDEAAAFIANDLLTSIETSSDEECAFCDEDQESPVPWA SLFALQTENGFWKLTPELGLILNLNVNALLTSLEEKGIRSLGTKGRERLLDLIATLLVLQFLYTKLEQEG MVAKSLIKMDDAFISRNIPWAFENIKKAREWARKTEGQYPSICQRLELGKDWESATKQLLGIQPQANTSL HRILYYSQG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145978 |
ORF Size | 5907 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145978.2, NP_001139450.2 |
RefSeq Size | 6391 bp |
RefSeq ORF | 5910 bp |
Locus ID | 328417 |
UniProt ID | E9PYK3 |
Gene Summary | Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function] |
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