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Mark1 (NM_145515) Mouse Tagged ORF Clone
CAT＃: MG215783
- TrueORF®
Mark1 (tGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1), (10ug)
ORF Plasmid: DDK
"NM_145515" in other vectors (4)
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CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW491150; B930025N23Rik; Emk3; mKIAA1477; mPar-1c; Par-1c |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215783 representing NM_145515
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGCGGACGCCATTGCCCACGGTGAACGAGCGGGACACGGAGAACCATACGTCTGTGGATGGCT ACACTGAAACACATATCCCGCCTGCCAAGTCAAGTAGCAGACAGAACCTTCCCCGGTGTAGAAACTCCAT TACGTCAGCGACAGATGAACAGCCTCACATCGGAAATTACCGCTTACAGAAAACAATAGGGAAAGGAAAT TTTGCCAAAGTGAAATTGGCAAGACATGTTCTAACAGGTAGAGAGGTCGCTGTGAAAATAATAGACAAAA CTCAGCTAAACCCTACCAGTCTACAGAAGTTGTTCCGAGAAGTACGAATAATGAAGATACTGAACCATCC TAACATAGTGAAACTGTTTGAAGTTATTGAAACAGAGAAGACACTCTATTTGGTCATGGAATACGCAAGT GGGGGTGAAGTCTTTGATTACTTAGTTGCCCACGGAAGAATGAAAGAGAAAGAGGCCCGTGCCAAATTCA GGCAGATTGTATCTGCTGTACAGTATTGCCATCAAAAATGCATTGTTCACCGTGATCTTAAGGCTGAAAA CCTTCTCCTTGATGCTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGCAATGAATTCACAGTGGGG AACAAATTGGACACATTCTGTGGAAGCCCGCCTTATGCTGCCCCCGAGCTTTTCCAAGGCAAGAAGTACG ATGGGCCAGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTACACGCTGGTCAGTGGCTCGCTGCCGTT TGATGGCCAGAATCTAAAGGAACTTCGGGAGCGAGTCCTTCGAGGGAAGTACCGCATCCCCTTCTACATG TCCACAGACTGTGAGAATCTCCTGAAGAAACTGTTAGTGCTGAATCCCATAAAGCGGGGCAGCCTGGAGC AAATAATGAAAGACCGATGGATGAATGTTGGTCATGAGGAAGAAGAGCTAAAGCCGTACTCTGAGCCTGA GCTTGATCTCAGCGATGCAAAAAGAATAGACATTATGGTCACCATGGGTTTTGCACGAGATGAAATCAAT GATGCCTTAGTAAGTCAGAAATATGATGAAGTTATGGCTACTTACATCCTTCTAGGTAGAAAACCACCCG AGTTCGAAGGTGGTGAGTCACTGTCCAGTGGGAGCTTGTGCCAGCGGTCGCGGCCGAGCAGCGACCTGAA CAACAGCACCCTTCAGTCGCCTGCTCACCTGAAGGTCCAGCGGAGCATCTCGGCCAACCAGAAGCAGAGG CGCTTCAGCGACCACGCCGGCCCCTCCATCCCCCCTGCCGTCTCATACACCAAGAGGCCTCAGGCCAACA GCGTGGAGAGTGAACAGAAGGAGGAGTGGGGCAAAGACACAGCCCGCAGACTTGGCAGCACCACAGTGGG ATCAAAGAGCGAAGTCACAGCCAGCCCCTTGGTGGGGCCAGACAGGAAAAAGTCCACAGCGAGCCCAAGT AACAACGTATATTCTGGCGGTAGCATGGCGAGGAGGAATACGTATGTGTGTGAGAGGAGCACAGACCGGT ACGCAGCCTTGCAGAATGGAAGAGACAGCAGCCTTACAGAGATGTCTGCCAGCAGCATGTCCTCGGCGGG CTCCACCGTGGCCTCTGCTGGCCCCTCAGCACGTCCCCGCCACCAGAAATCTATGTCAACCTCTGGCCAC CCTATAAAAGTTACACTGCCAACCATTAAAGATGGCTCAGAAGCGTATCGGCCTGGTGCAGCTCAGAGAG TGCCTGCTGCTTCTCCGTCTGCTCACAGCATCAGTGCCAGCACTCCAGACAGGACCCGCTTCCCCCGAGG GAGCTCCAGCCGAAGCACTTTCCATGGGGAACAGCTCCGAGAGCGGCGCAGCGCGGCTTACAACGGACCA CCGGCCTCCCCATCCCACGAAACGGGTGCGTTTGCACATGCCAGAAGGGGCACGTCAACCGGCATCATCA GCAAAATCACCTCCAAGTTCGTCCGCAGGGATCCAAGTGAAGGCGAAGCCAGCGGCAGAGCCGACACAGC CAGAGGTTCATCGGGAGATCCCAAAGAGAGAGACAAGGACGAGGGCAAAGAGGCCAAGCCCCGCTCCCTG CGCTTCACCTGGAGCATGAAGACCACGAGCTCCATGGACCCCAACGACATGCTGAGGGAGATCCGCAAGG TGCTGGATGCCAACACCTGTGACTATGAGCAGAAAGAGAGGTTCCTGCTCTTCTGCGTTCACGGAGACGC TCGGCAGGACAGCCTCGTACAGTGGGAGATGGAGGTCTGCAAGTTGCCTCGGCTCTCACTCAACGGGGTC CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCCAAAATAGCAAATGAGCTTA AGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215783 representing NM_145515
Red=Cloning site Green=Tags(s) MSARTPLPTVNERDTENHTSVDGYTETHIPPAKSSSRQNLPRCRNSITSATDEQPHIGNYRLQKTIGKGN FAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYAS GGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKCIVHRDLKAENLLLDADMNIKIADFGFSNEFTVG NKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYM STDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYSEPELDLSDAKRIDIMVTMGFARDEIN DALVSQKYDEVMATYILLGRKPPEFEGGESLSSGSLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQR RFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWGKDTARRLGSTTVGSKSEVTASPLVGPDRKKSTASPS NNVYSGGSMARRNTYVCERSTDRYAALQNGRDSSLTEMSASSMSSAGSTVASAGPSARPRHQKSMSTSGH PIKVTLPTIKDGSEAYRPGAAQRVPAASPSAHSISASTPDRTRFPRGSSSRSTFHGEQLRERRSAAYNGP PASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRADTARGSSGDPKERDKDEGKEAKPRSL RFTWSMKTTSSMDPNDMLREIRKVLDANTCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGV RFKRISGTSIAFKNIASKIANELKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145515 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145515.2, NP_663490.2 |
| RefSeq Size | 4565 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 226778 |
| UniProt ID | Q8VHJ5 |
| Gene Summary | Serine/threonine-protein kinase (By similarity). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221734 | Mark1 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1), (10ug) |
CNY 10,260.00 |
|
| MR215783 | Mark1 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1) |
CNY 5,824.00 |
|
| MR215783L3 | Lenti ORF clone of Mark1 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1) |
CNY 8,930.00 |
|
| MR215783L4 | Lenti ORF clone of Mark1 (mGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1) |
CNY 8,930.00 |
