Eya4 (NM_010167) Mouse Tagged ORF Clone
CAT#: MG215832
- TrueORF®
Eya4 (tGFP-tagged) - Mouse eyes absent 4 homolog (Drosophila) (Eya4), (10ug)
ORF Plasmid: DDK
"NM_010167" in other vectors (4)
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CNY 6,840.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B130023L16Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215832 representing NM_010167
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACACCCAGGACCTAAATGAGCAATCTGTAAAGAAAACGTGCCCAGAAGCAGATGTTTCGGAAC CTCAGAATTCCAGGTCTATGGAAATGCAAGACCTAGCAAGTCCCCATGCCTTGGTTGGAGGCAGTGATAC ACCTGGGAGCTCCAAGCTGGATAAATCTGGTCTCAGCAGCACTTCAGTCACAACAAATGGGACAGGAGTG TCTCTTCTTGCAGTCAAAACAGAGCCCTTGCACAGCAGTGAGTCCACTACCACGACTGGAGATGGAGCGC TTGACACTTTTACTGGGTCAGTAATAACAAGCAGCGGCTACAGCCCCAGGTCGGCGCAGCAGTACTCTCC ACAGCTGTACCCCTCCAAGCCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCGGCCTAT GCAGGCCAGACTCAGTACTCTGGGATGCAGCAGCCGGCCGTCTACACAGCCTACTCGCAGACAGGACAGC CCTACAGCCTGCCTGCCTACGATCTTGGTGTGATGTTGCCAGCCATCAAGACGGAAAGTGGGCTTTCCCA AACTCAGTCCCCGTTACAGAGTGGCTGCCTGAGCTACAGCCCTGGGTTTTCTACCCCACAGCCAGGCCAG ACGCCTTACTCTTACCAAATGCCAGGTTCCAGCTTTGCACCATCGTCTACTATTTATGCAAATAATTCCG TTTCTAATTCAACAAACTTCAGCAGCTCACAGCAGGATTACCCATCCTACACAGCCTTTGGCCAAAACCA ATATGCTCAGTATTACTCAGCATCAACCTATGGGGCATACATGACATCAAACAACACAGCTGACGGCACA TCATCGTCAACATCAACTTATCAGCTGCAGGAATCTCTCCAAGGACTGACTAGCCAACCAGGAGAGTTTG ATACAGTGCAGAGTCCCTCCACACCAATCAAAGATCTCGATGACAGAACATGTAGAAGTTCTGGGTCAAA GTCTCGAGGAAGAGGCCGGAAAAATAATCCCTCCCCACCTCCGGACAGTGACCTAGAGCGTGTGTTTGTC TGGGACTTGGATGAAACCATCATCGTCTTCCACTCTCTCCTCACGGGCTCTTATGCACAGAAGTATGGCA AGGATCCCCCAATGGCTGTGACTCTCGGGCTCCGGATGGAAGAAATGATCTTTAATCTTGCAGATACACA TTTGTTTTTTAATGACTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTTCCTCCGACGACAATGGT CAGGACTTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCGAACCTTTGTT TGCCAACAGGTGTGAGAGGAGGAGTGGACTGGATGAGGAAGCTGGCTTTTCGTTACAGAAGAGTAAAGGA ATTATATAACACCTACAAGAACAATGTTGGAGGACTCCTTGGCCCTGCTAAGAGGGATGCATGGCTGCAG TTAAGGGCAGAGATCGAAGGCCTAACAGATTCCTGGCTAACGAATGCACTTAAGTCTTTATCAATTATTA GTACTAGGAGTAACTGTGTAAATGTCTTGGTAACGACAACCCAGCTGATTCCTGCTCTTGCAAAAGTCCT GCTCTACAGCCTAGGAGGTGCTTTCCCCATTGAGAACATTTACAGCGCAACAAAAATAGGAAAAGAAAGT TGCTTTGAGCGAATAATGCAAAGGTTTGGCAGAAAAGTAGTGTATGTTGTAATTGGGGATGGTGTAGAAG AAGAGCAGGCAGCAAAAAAGCACAACATGCCCTTTTGGAGGATATCAAGCCACTCGGACCTCTTGGCTCT ACATCAAGCACTGGAATTAGAGTATTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215832 representing NM_010167
Red=Cloning site Green=Tags(s) MEDTQDLNEQSVKKTCPEADVSEPQNSRSMEMQDLASPHALVGGSDTPGSSKLDKSGLSSTSVTTNGTGV SLLAVKTEPLHSSESTTTTGDGALDTFTGSVITSSGYSPRSAQQYSPQLYPSKPYPHILSTPAAQTMSAY AGQTQYSGMQQPAVYTAYSQTGQPYSLPAYDLGVMLPAIKTESGLSQTQSPLQSGCLSYSPGFSTPQPGQ TPYSYQMPGSSFAPSSTIYANNSVSNSTNFSSSQQDYPSYTAFGQNQYAQYYSASTYGAYMTSNNTADGT SSSTSTYQLQESLQGLTSQPGEFDTVQSPSTPIKDLDDRTCRSSGSKSRGRGRKNNPSPPPDSDLERVFV WDLDETIIVFHSLLTGSYAQKYGKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNG QDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRVKELYNTYKNNVGGLLGPAKRDAWLQ LRAEIEGLTDSWLTNALKSLSIISTRSNCVNVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKES CFERIMQRFGRKVVYVVIGDGVEEEQAAKKHNMPFWRISSHSDLLALHQALELEYL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010167 |
ORF Size | 1848 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010167.4, NP_034297.2 |
RefSeq Size | 4099 bp |
RefSeq ORF | 1851 bp |
Locus ID | 14051 |
Gene Summary | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219697 | Eya4 (untagged) - Mouse eyes absent 4 homolog (Drosophila) (Eya4), (10ug) |
CNY 7,890.00 |
|
MR215832 | Eya4 (Myc-DDK-tagged) - Mouse eyes absent 4 homolog (Drosophila) (Eya4) |
CNY 6,180.00 |
|
MR215832L3 | Lenti ORF clone of Eya4 (Myc-DDK-tagged) - Mouse eyes absent 4 homolog (Drosophila) (Eya4) |
CNY 8,080.00 |
|
MR215832L4 | Lenti ORF clone of Eya4 (mGFP-tagged) - Mouse eyes absent 4 homolog (Drosophila) (Eya4) |
CNY 8,080.00 |