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Ppm1e (NM_177167) Mouse Tagged ORF Clone
CAT＃: MG216200
- TrueORF®
Ppm1e (tGFP-tagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e), (10ug)
ORF Plasmid: DDK
"NM_177167" in other vectors (4)
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CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW049266; B930008A12Rik; mKIAA1072; POPX1; PP2CH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216200 representing NM_177167
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTGTTCCTGGGCGAGTTCCGCG GACCGTGCGGTGGCGGCGAGCCAGAGCCCGAACCCGAATCCGAGCCCGAGCCCGAGCCCGAAGCCGAGCT AGTCGCGGCTGAGGCGGCCGAGGCTTCGGGGGAGGAACCGGGGGAGGACGCTGCGACGGTAGAGGCGACC GAGGAGGGGGAGCAGGACCAAGACCCCGAGCCCGAAGACGAGGCGGTGGAGGAGGAGACGGCGACGGAGG GCGAGGAGGAGGAAGAGGAGGAAGCGGCTGCCCCGGGGCACTCGGCCGTGCCGCCACCGCCGCAGCCCCA GCTGCCACCGCTGCCCCCGCTCCCACGGCCGCTGTCGGAGCGCATCACCCGAGAGGAGGTGGAGGGCGAG AGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAACTGCCCTTCCTTTCTGGCTGCTGCTTTAGCCA GAGCCACATCAGATGAAGTCCTCCAGAGTGACCTTTCTGCTCACTGTATCCCAAAGGAAACGGACGGCAC AGAAGGGACTGTGGAAATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACATGAGATCTGC TGCAGTTGGGTGAAGGACTTCCCTCTGCGCAGGAGGCCTCAGATTTATTATGAGACGTCAATTCATGCCA TCAAGAACATGCGAAGGAAAATGGAGGACAAACACGTCTGCATTCCTGACTTTAACATGCTCTTCAACCT AGAGGACCAGGAGGAACAAGCATACTTTGCAGTGTTTGATGGCCATGGGGGAGTGGACGCTGCTATTTAT GCCTCCGTTCACCTACACGTTAACTTAGTGCGCCAGGAGATGTTTCCCCATGATCCTGCTGAGGCTCTCT GCCGGGCCTTCCGTGTCACAGATGAGCGGTTTGTGCAGAAGGCAGCCAGGGAGAGCTTACGGTGTGGGAC CACAGGAGTAGTGACTTTTATCAGAGGCAACATGCTCCACGTGGCATGGGTGGGCGATTCCCAGGTCATG CTGGTAAGAAAGGGGCAAGCTGTTGAACTAATGAAACCACACAAGCCAGACAGAGAGGATGAAAAGCAGC GAATTGAGGCCCTTGGAGGCTGTGTGGTTTGGTTTGGCGCCTGGAGGGTGAATGGAAGCCTGTCCGTCTC CAGAGCCATCGGAGATGCTGAGCACAAGCCGTATATCTGTGGAGACGCAGACTCGGCCTCCACTGTTTTG GATGGGACTGAAGACTACCTCATTCTGGCCTGTGACGGCTTCTATGACACCGTGAACCCTGACGAGGCAG TGAAGGTTGTGTCTGACCACTTGAAAGAGAATAACGGAGACAGCAGCATGGTTGCCCACAAATTAGTAGC GTCGGCTCGTGATGCGGGGTCAAGTGATAACATCACTGTTATTGTGGTATTCCTGAGGGACATGAACAAG GCTGTAAATGTTAGTGAGGAGTCAGAGTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGG ATGATAAGGAGACTCATGGAGAGTGCAAGCGCCCTTGGCCACAGCACCAGTGCTCAGCACCCGCCGATCT AGGCTATGAGGGGCGAGTGGATTCATTCACTGATAGAACTAGCCTCAGCCCAGGGCCCCAAATCAACGTG CTGGAAGATCCAGACTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTACCCAATTTTTGC CACCAGTTGAGATGATTGGTCCTGGTGCACCAAAGAAAGATCTTAATGAGCTAATAATGGAGGAGAGATC AGTCAAGTCATCATTGCCTGAACGGAGTGGTGCTGGAGAGCCTCGGGTTTCTTTTAATTTGGGTTCAACA GGGCAACAGATATGCAGAATGGAGAACTTGTCTCCTGTCAGTTCTGGGTTGGAAAATGAACAGTTCAAAT CCCGGGGGAAGACAGCGTCTAGGTTGTATCATTTACGCCACCACTACTCCAAAAGGCAGCGTGGATTCAG GTTTAATCCAAAGTTTTACTCGTTTCTGTCTGCTCGAGAGCCTTCTCACAAAATAGGCATTAGCCTCTCC TCACTTACTCGAAGTGGGAAGAGAAACAAGATGTTAAGAAGTTCTTTGCCATGGAGGGAAAATAGTTGGG AAGGATATAGTGGAAATGTGAAGATCAGAAAGCGTAATGATATTCCATGCCCAGACTTTCCCTGGAGCTA TAAAATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216200 representing NM_177167
Red=Cloning site Green=Tags(s) MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPESEPEPEPEAELVAAEAAEASGEEPGEDAATVEAT EEGEQDQDPEPEDEAVEEETATEGEEEEEEEAAAPGHSAVPPPPQPQLPPLPPLPRPLSERITREEVEGE SLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHCIPKETDGTEGTVEIETVKLARSVFSKLHEIC CSWVKDFPLRRRPQIYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIY ASVHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVM LVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVL DGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNK AVNVSEESEWTENSFQGGQEDGGDDKETHGECKRPWPQHQCSAPADLGYEGRVDSFTDRTSLSPGPQINV LEDPDYLDLTQIEASKPHSTQFLPPVEMIGPGAPKKDLNELIMEERSVKSSLPERSGAGEPRVSFNLGST GQQICRMENLSPVSSGLENEQFKSRGKTASRLYHLRHHYSKRQRGFRFNPKFYSFLSAREPSHKIGISLS SLTRSGKRNKMLRSSLPWRENSWEGYSGNVKIRKRNDIPCPDFPWSYKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177167 |
| ORF Size | 2247 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177167.4, NP_796141.2 |
| RefSeq Size | 6315 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 320472 |
| UniProt ID | Q80TL0 |
| Gene Summary | Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42 (By similarity). Dephosphorylates PRKAA2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221341 | Ppm1e (untagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e), (10ug) |
CNY 5,496.00 |
|
| MR216200 | Ppm1e (Myc-DDK-tagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e) |
CNY 5,496.00 |
|
| MR216200L3 | Lenti ORF clone of Ppm1e (Myc-DDK-tagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e) |
CNY 8,460.00 |
|
| MR216200L4 | Lenti ORF clone of Ppm1e (mGFP-tagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e) |
CNY 8,460.00 |
