United States
Germany
Japan
United Kingdom
China
Map4k1 (NM_008279) Mouse Tagged ORF Clone
CAT＃: MG217292
- TrueORF®
Map4k1 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), (10ug)
ORF Plasmid: DDK
"NM_008279" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Hpk1; mHPK1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217292 representing NM_008279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCGAGAACACTATGATCTACTGCAACGACTGG GTGGTGGAACTTATGGAGAAGTATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGAT GGTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCTCATGCTGAAAACTTGCCGG CACGCCAACATCGTGGCTTACCATGGCAGTTACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCT GTGGAGCTGGTTCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGATCAGCTATGT GTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAGAGAAGAAGATACATCGAGACATCAAGGGA GCCAACATCCTCATCAACGACTGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGG CGACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTCCAGAGGTGGCAGCGGTGGC CCTGAAGGGTGGATACAATGAGCTGTGCGACATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAG CTTCAGCCCCCTCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCGGGTACCAGC CTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTCCACAACTTTGTCAAAGTCACCCTCACTAA GAACTCCAAGAAACGCCCCAGTGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAAC AGAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGGCTGCCTGTTGATATTGAAG ATGAGGAGCCTGAGCCACCCCCTGCCATCCCTCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGG GATCCCAGATGCAGACTGCTGCCGGAGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGACTGCAGACCT CAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGGGAGCACCAGTCCCAGGAGTCAGCTGTCAG ATTCTGATGACGACTATGATGATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCC CAAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGACGATGGGCAATTGAGCCCA GGGGTTCTGGTTCGATGTGCAAGTGGGCCCCCTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCT GCAGCCCTCACCTCACAGCTCGCTCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCCAGGCCAGCC CCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGATGGAGAACGAGAAGCGCAGAGAGAAATAT CCCCTTCTTGTGAAATTATTCAATGGCTGTCCCCTTCAGATCCACAGCACAGCTGCCTGGACACATCCCT CTACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATACTGAACCGGAATGATCAGGA GGCCACGCTGGAAATGATCTTCCCTGGCCGCACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCT CTCTCAGGGAAGACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAGACGGCAGAA CGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAAGAAAGAACATGGTCTCCAGTAAGAT CCAGGACACCAAAGGCTGCCGGGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCCATTCTTATGT GGGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAACAAATTCTTGCTTGTCCGGC AGGTGCTGTTCCCGCTGCCCACGCCGCTGCCGGTGTTCACGCTGCTGACAACGCCGGGCTCCGAGCTGCC AGCTGTGTGCATCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGTGCGCTTCGGT GCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAAGGGACCAGTGCAGGTGATCCAAGTAAAGG AAGACATGGTGATGGTGTTGATGGATGGCTCTCTAAAGCTAGTGACCCCAGAGGGGGCACCAGCTCCTGG GCTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCTGTGGCTATGGTTGAAGATCGACTCGAG GCCTTCTGGAAGCATGGAGTTCAGGTGTGGGCTCCAGGCTTGAAACAGCCGCTGCAGGAGCTGAGAGACC CCACCCTCACCTTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGTGGTGGAGACAAGGCCCACGGATGACCC TACGGCCCCCAGCAACCTCTACATCCAGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217292 representing NM_008279
Red=Cloning site Green=Tags(s) MALVDPDIFNKDPREHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCR HANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSEKKIHRDIKG ANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAE LQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPGLN RGLILDLLDKMKNPGKGLPVDIEDEEPEPPPAIPRRIRSTYRASSLGIPDADCCRRQMEFQRPRCVDCRP QAETVRLYPPAHFGSTSPRSQLSDSDDDYDDVDIPAPSENIPPPLPPKPKFRSPSDDGSGGIRDDGQLSP GVLVRCASGPPPRTPRPGPPPATCSPHLTARSDPSLWNPAAPEPGQPPLVPPRKEKMRGKMENEKRREKY PLLVKLFNGCPLQIHSTAAWTHPSTKDQNLLLGAEEGIFILNRNDQEATLEMIFPGRTTWLYCINNLLMS LSGKTPYLYSHSILGLLERKDGRTGSPIAHISPHRLLARKNMVSSKIQDTKGCRACCVAESASSGGPFLC GALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLPVFTLLTTPGSELPAVCIGVSPGQAAKSVLFHTVRFG ALSCWLDDSSTEHKGPVQVIQVKEDMVMVLMDGSLKLVTPEGAPAPGLRTPEIPMTEAVEAVAMVEDRLE AFWKHGVQVWAPGLKQPLQELRDPTLTFRLLCSPRPVVVETRPTDDPTAPSNLYIQE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008279 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008279.2, NP_032305.2 |
| RefSeq Size | 2699 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 26411 |
| UniProt ID | P70218 |
| Gene Summary | Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221868 | Map4k1 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), (10ug) |
CNY 10,550.00 |
|
| MR217292 | Map4k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1) |
CNY 8,270.00 |
|
| MR217292L3 | Lenti ORF clone of Map4k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1) |
CNY 10,170.00 |
|
| MR217292L4 | Lenti ORF clone of Map4k1 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1) |
CNY 10,170.00 |
