Lnx1 (NM_001159580) Mouse Tagged ORF Clone
CAT#: MG217668
- TrueORF®
Lnx1 (tGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001159580" in other vectors (4)
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CNY 6,840.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Lnx |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217668 representing NM_001159580
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCGCTGCTGCTTCTGGTCCTGCCCTGGCTGAGTCCAGCTAACTACATTGACAATGTGGGCAACC TGCACTTCCTGTACTCGGAACTCTGCAAAGGGGCCTCCCACTATGGCCTGACCAAGGACAGGAAAAGGCG CTCTCAAGATGGCTGTCCTGATGGTTGCGCCAGCCTCATGGCAACGACGCTCTCCCCAGAGGTTTCTGCA GCCGCCACCATCTCTTTAATGACAGATGAGCCTGGCCTAGACAACCCTGCCTACGTGTCCTCGGTGGAGG ATGGTGAGCCAGTAGCCAACTCCTCAGACTCTGGCCGGAGCAACCGGACTAGGGCACGGCCCTTTGAGCG CTCCACTATGAGAAGCCGGTCTTTCAAGAAGATCAACCGAGCTCTAAGTGCTCTCCGGAGGACGAAGAGC GGGAGTGTCGTTGCCAACCATGTTGACCAGGGCAGGGACAACTCTGAGAACACCACTGTCCCCGAAGATG GTGAAATTACAAGCATCAAAATCAACAGAGCGGATCCCAGCGAAAGCCTCTCCATAAGGCTGGTGGGGGG CAGCGAGACGCCGCTGGTCCACATCATCATCCAGCATATTTATCGCGATGGAGTGATTGCCAGAGATGGA CGGCTGCTGCCAGGAGACATCATTCTCAAGGTCAACGGCATGGACATCAGCAATGTCCCTCATAACTATG CCGTGCGGCTCCTGCGGCAGCCCTGCCAGGTGCTGCGGCTAACGGTGCTGCGGGAGCAGAAGTTCCGTAG CAGAAGCAATGCGCACGTGCCGGACTCCTATGGACCTCGGGATGACAGCTTCCATGTAATCCTCAACAAA AGCAGCCCCGAGGAGCAGCTTGGCATAAAGCTGGTGCGCCGGGTGGATGAGCCCGGCGTGTTCATCTTCA ACGTGCTAAATGGGGGTGTCGCCGACCGACACGGCCAGCTGGAGGAGAATGACCGTGTGCTGGCCATCAA CGGGCATGACCTTCGATTTGGCAGTCCAGAGAGTGCAGCTCATCTGATTCAGGCCAGTGAAAGACGCGTC CATCTCGTCGTGTCCCGCCAGGTTCGACAGTCCAGCCCAGACATCTTTCAAGAAGCTGGCTGGATCAGCA ATGGCCAGCAATCCCCAGGCCCAGGAGAGAGGAACACAGCTTCTAAGCCTGCAGCCACCTGTCATGAGAA GGTTGTAAGTGTCTGGAAAGACCCCAGCGAGTCTCTCGGCATGACCGTCGGAGGGGGAGCATCCCACAGG GAATGGGACTTGCCCATCTATGTCATCAGTGTTGAACCCGGCGGAGTCATAAGCAGAGACGGGAGAATAA AAACAGGTGACATTTTATTGAATGTGAATGGGATTGAGCTCACAGAGGTCAGCCGGACAGAGGCCGTTGC CATACTGAAGAGCGCACCCTCCTCAGTGGTACTCAAAGCCTTGGAAGTCAAGGAACAGGAGGCCCAGGAA GACTGCAGCCCAGCAGCCCTGGACTCCAACCACAACGTGACCCCGCCTGGTGACTGGTCCCCATCCTGGG TCATGTGGCTGGAATTACCACAGTACTTATGTAACTGTAAAGATGTGATACTGCGAAGGAACACAGCTGG AAGCCTGGGCTTCTGCATTGTGGGAGGTTATGAAGAATACAGTGGGAACAAACCTTTTTTTATCAAGTCC ATTGTTGAAGGAACACCTGCATACAATGACGGAAGAATCAGATGTGGTGACATTCTTCTCGCTGTCAACG GTAGAAGTACATCGGGTATGATACACGCTTGCCTGGCCAGGATGCTCAAGGAACTTAAAGGGAGAATTAC TCTGACCATTGCTTCTTGGCCTGGTACTTTTTTA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217668 representing NM_001159580
Red=Cloning site Green=Tags(s) MKALLLLVLPWLSPANYIDNVGNLHFLYSELCKGASHYGLTKDRKRRSQDGCPDGCASLMATTLSPEVSA AATISLMTDEPGLDNPAYVSSVEDGEPVANSSDSGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKS GSVVANHVDQGRDNSENTTVPEDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDG RLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAHVPDSYGPRDDSFHVILNK SSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRV HLVVSRQVRQSSPDIFQEAGWISNGQQSPGPGERNTASKPAATCHEKVVSVWKDPSESLGMTVGGGASHR EWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSAPSSVVLKALEVKEQEAQE DCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTAGSLGFCIVGGYEEYSGNKPFFIKS IVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159580 |
ORF Size | 1854 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159580.1, NP_001153052.1 |
RefSeq Size | 2317 bp |
RefSeq ORF | 1857 bp |
Locus ID | 16924 |
UniProt ID | O70263 |
Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219704 | Lnx1 (untagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 5, (10ug) |
CNY 7,890.00 |
|
MR217668 | Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 5 |
CNY 6,180.00 |
|
MR217668L3 | Lenti ORF clone of Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 5 |
CNY 8,080.00 |
|
MR217668L4 | Lenti ORF clone of Lnx1 (mGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 5 |
CNY 8,080.00 |