United States
Germany
Japan
United Kingdom
China
Actr3 (NM_023735) Mouse Tagged ORF Clone
CAT＃: MG217895
- TrueORF®
Actr3 (tGFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), (10ug)
ORF Plasmid: DDK
"NM_023735" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200003A09Rik; Arp3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217895 representing NM_023735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGATATACAAAACTAGGATATGCTGGAA ATACAGAGCCACAGTTTATCATCCCATCATGTATTGCCATTAAAGAGTCTGCAAAAGTGGGTGACCAAGC CCAGAGGAGGGTGATGAAAGGCGTGGATGACCTAGACTTCTTCATTGGTGATGAAGCAATAGAAAAGCCC ACATATGCAACAAAGTGGCCAATTCGCCATGGTATAGTTGAAGACTGGGACTTAATGGAAAGGTTTATGG AGCAAGTGATTTTTAAATATTTAAGGGCAGAACCTGAAGATCATTACTTTCTTTTGACTGAACCTCCACT GAATACTCCAGAAAACAGGGAATATACTGCTGAAATAATGTTTGAATCCTTCAATGTTCCAGGCTTGTAC ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCCTGGACCTCAAGACAAGTAGGAGAGCGGACGCTGA CGGGTACAGTAATAGACAGTGGAGACGGAGTCACTCATGTCATTCCTGTGGCTGAAGGATATGTTATCGG CAGCTGTATTAAACACATTCCAATCGCAGGAAGAGATATAACATATTTTATTCAGCAACTGCTGCGAGAC CGAGAAGTAGGAATCCCTCCTGAGCAGTCCTTGGAAACTGCGAAAGCAGTGAAGGAACGCTACAGTTATG TCTGCCCAGATTTAGTAAAAGAGTTTAACAAGTATGACACCGATGGGTCAAAGTGGATCAAACAGTACAC CGGAGTCAACGCCATCTCAAAGAAAGAGTTTTCTATTGATGTTGGCTATGAGCGATTCCTGGGACCCGAG ATCTTTTTCCATCCAGAGTTTGCTAATCCAGATTTTACACAACCTATCTCAGAAGTTGTAGATGAAGTCA TTCAGAATTGCCCCATTGATGTCCGGCGTCCTCTCTACAAGAACATTGTCCTCTCTGGTGGTTCAACCAT GTTCAGGGACTTTGGACGTCGTTTGCAAAGAGATTTGAAGAGAACTGTAGATGCCAGGCTGAAGTTAAGC GAGGAGCTGAGTGGTGGTAGATTGAAGCCCAAGCCTATTGATGTACAAGTTATTACACACCATATGCAGC GGTATGCAGTCTGGTTTGGAGGGTCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAA AAAGGATTATGAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217895 representing NM_023735
Red=Cloning site Green=Tags(s) MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKP TYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLY IAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRD REVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGVNAISKKEFSIDVGYERFLGPE IFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLS EELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023735 |
| ORF Size | 1254 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023735.2, NP_076224.1 |
| RefSeq Size | 2746 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 74117 |
| UniProt ID | Q99JY9 |
| Gene Summary | ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR217895 | Actr3 (Myc-DDK-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1 |
CNY 2,850.00 |
|
| MR217895L1 | Lenti ORF clone of Actr3 (Myc-DDK-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1 |
CNY 4,750.00 |
|
| MR217895L2 | Lenti ORF clone of Actr3 (mGFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1 |
CNY 4,750.00 |
|
| MR217895L3 | Lenti ORF clone of Actr3 (Myc-DDK-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1 |
CNY 4,750.00 |
|
| MR217895L4 | Lenti ORF clone of Actr3 (mGFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), transcript variant 1 |
CNY 4,750.00 |
