Dgat2 (NM_026384) Mouse Tagged ORF Clone
CAT#: MG218673
- TrueORF®
Dgat2 (tGFP-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2), (10ug)
ORF Plasmid: DDK
"NM_026384" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,088.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0610010B06Rik; ARAT; DGAT-2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218673 representing NM_026384
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACCCTCATCGCCGCCTACTCCGGGGTCCTGCGGGGTGAGCGTCGGGCGGAAGCTGCCCGCAGCG AAAACAAGAATAAAGGATCTGCCCTGTCACGCGAGGGGTCTGGGCGATGGGGCACTGGCTCCAGCATCCT CTCAGCCCTCCAAGACATCTTCTCTGTCACCTGGCTCAACAGATCTAAGGTGGAAAAACAGCTGCAGGTC ATCTCAGTACTACAATGGGTCCTATCCTTCCTGGTGCTAGGAGTGGCCTGCAGTGTCATCCTCATGTACA CCTTCTGCACAGACTGCTGGCTGATAGCTGTGCTCTACTTCACCTGGCTGGCATTTGACTGGAACACGCC CAAGAAAGGTGGCAGGAGATCGCAGTGGGTGCGAAACTGGGCCGTGTGGCGCTACTTCCGAGACTACTTT CCCATCCAGCTGGTGAAGACACACAACCTGCTGACCACCAGGAACTATATCTTTGGATACCACCCCCATG GCATCATGGGCCTGGGTGCCTTCTGTAACTTCAGCACAGAGGCTACTGAAGTCAGCAAGAAGTTTCCTGG CATAAGGCCCTATTTGGCTACGTTGGCTGGTAACTTCCGGATGCCTGTGCTTCGCGAGTACCTGATGTCT GGAGGCATCTGCCCTGTCAACCGAGACACCATAGACTACTTGCTCTCCAAGAATGGGAGTGGCAATGCTA TCATCATCGTGGTGGGAGGTGCAGCTGAGTCCCTGAGCTCCATGCCTGGCAAGAACGCAGTCACCCTGAA GAACCGCAAAGGCTTTGTGAAGCTGGCCCTGCGCCATGGAGCTGATCTGGTTCCCACTTATTCCTTTGGA GAGAATGAGGTATACAAGCAGGTGATCTTTGAGGAGGGTTCCTGGGGCCGATGGGTCCAGAAGAAGTTCC AGAAGTATATTGGTTTCGCCCCCTGCATCTTCCATGGCCGAGGCCTCTTCTCCTCTGACACCTGGGGGCT GGTGCCCTACTCCAAGCCCATCACCACCGTCGTGGGGGAGCCCATCACTGTCCCCAAGCTGGAGCACCCG ACCCAGAAAGACATCGACCTGTACCATGCCATGTACATGGAGGCCCTGGTGAAGCTCTTTGACAATCACA AGACCAAATTTGGCCTTCCAGAGACTGAGGTGCTGGAGGTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218673 representing NM_026384
Red=Cloning site Green=Tags(s) MKTLIAAYSGVLRGERRAEAARSENKNKGSALSREGSGRWGTGSSILSALQDIFSVTWLNRSKVEKQLQV ISVLQWVLSFLVLGVACSVILMYTFCTDCWLIAVLYFTWLAFDWNTPKKGGRRSQWVRNWAVWRYFRDYF PIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMS GGICPVNRDTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLKNRKGFVKLALRHGADLVPTYSFG ENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITVPKLEHP TQKDIDLYHAMYMEALVKLFDNHKTKFGLPETEVLEVN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_026384 |
ORF Size | 1164 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026384.3, NP_080660.1 |
RefSeq Size | 2251 bp |
RefSeq ORF | 1167 bp |
Locus ID | 67800 |
UniProt ID | Q9DCV3 |
Gene Summary | Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Host triacylglycerols shape the lipidome of intracellular trypanosomes and modulate their growth
,null,
PLoS Pathogens
,PubMed ID 29281741
[Dgat2]
|
Chlamydia trachomatis regulates growth and development in response to host cell fatty acid availability in the absence of lipid droplets
,Sharma, M;Recuero-Checa, MA;Fan, FY;Dean, D;,
Cell. Microbiol.2017
,PubMed ID 29117636
[DGAT2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210628 | Dgat2 (untagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2), (10ug) |
CNY 5,488.00 |
|
MR218673 | Dgat2 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) |
CNY 5,488.00 |
|
MR218673L3 | Lenti ORF clone of Dgat2 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) |
CNY 5,890.00 |
|
MR218673L4 | Lenti ORF clone of Dgat2 (mGFP-tagged) - Mouse diacylglycerol O-acyltransferase 2 (Dgat2) |
CNY 5,890.00 |