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Mtr (NM_001081128) Mouse Tagged ORF Clone
CAT＃: MG218828
- TrueORF®
Mtr (tGFP-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), (10ug)
ORF Plasmid: DDK
"NM_001081128" in other vectors (4)
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CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI894170; D830038K18Rik; MS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218828 representing NM_001081128
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAAACCCTGCAGGATGAGATTGAAGCCATTCTTCGGAAGAGGATCATGGTGCTAGACGGTGGGA TGGGGACCATGATCCAGCGGTACAAACTAAGTGAAGAACATTTCCAAGGTCAAGAGTTTAAAGATCACAG CAGGCCACTGAAAGGCAACAATGACATCTTAAGTATAACTCAGCCTGATATTATTTACCAGATTCATAAG GAATACTTGCTGGCTGGAGCAGATATCATCGAAACAAACACTTTCAGCAGTACTAGTATCGCCCAGGCTG ACTATGGCCTTGAACACTTGGCCTACCGGATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGA GGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGAGCTCTGGGTCCGACTAATAAGACACTT TCTGTCTCCCCATCTGTGGAAAGGCCAGATTATAGGAACATCACATTTGATGAGCTTGTTGACGCATACC AGGAGCAGGCCAAGGGACTGCTGGATGGCAGGGTCGACATCTTACTCATTGAAACAATTTTTGATACAGC TAATGCCAAAGCAGCCTTGTTTGCGATCCAGAACCTCTTTGAAGAGAATTATGCTCCTCCTCGGCCTATC TTTATTTCTGGGACCATTGTTGATAAAAGTGGAAGGACTCTTTCTGGGCAGACGGGAGAGGCTTTTGTCA CCAGCGTGTCTCATTCGGACCCCCTGTGCATTGGATTAAATTGTTCTCTGGGTGCAGCTGAAATGAGGCC TTTCATTGAAACAATTGGAAAATGTACAACAGCCTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAAC ACTTTTGGCGACTATGATGAAACACCATCCACGATGGCCACGCACCTGAAGGACTTTGCTGTGGATGGCT TGGTGAATATAGTTGGTGGATGCTGTGGTTCAACACCTGATCACATCAGGGAAATTGCGGAAGCTGTGAA AAAGTGTAAGCCAAGAGTCCCCCCTGCTAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCC TTCAGGATTGGACCATACACCAACTTTGTTAACATTGGAGAGCGCTGTAATGTGGCAGGATCCAGGAAAT TTGCTAAACTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCATTGCCAAAGCACAGGTGGAAATGGG AGCTCAGGTGCTCGATATCAACATGGACGACGGCATGCTGGATGGTCCCAGTGCAATGACCAGATTTTGC AACTCCATTGCTTCTGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCTAACTTTGCCGTGA TTGAAGCTGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTCAAGGAGGGGGAGGG GGACTTCCTGGAGAAGGCCCGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAG GAAGGACAAGCAACAGAAACAGATGTTAAAGTCAATGTGTGCACCAGAGCTTACCATCTACTTGTGGACA AAGTGGGCTTCAATCCAAATGACATCATCTTTGACCCCAACATCCTTACCATTGGTACTGGAATGGAGGA ACATAACTTGTATGCTATCAATTTTATCCATGCAACAAGAGTCATTAAGGAAACCTTACCAGGAGTCAGA ATAAGTGGAGGTCTTTCCAACTTATCCTTCTCCTTCCGAGGGATGGAAGCCATTCGAGAAGCAATGCATG GTGTGTTCCTTTATCACGCGATCAAGTTTGGTATGGACATGGGGATAGTCAATGCCGGCAACCTCCCTGT GTATGATGCTATCCACAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAAAGACTCTGAGGCT ACTGAGAAACTCTTGCGATATGCCCAGACTCATGGCACAGGAGGGAAGAAAGTCATCCAGACAGATGAGT GGAGGAATGGCTCCATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGA AGATACTGAAGAAGCCAGGTTAAACGGGGAAAAATACCCTCGGCCTCTGAATATAATTGAAGGGCCCCTA ATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAGATGTTTCTACCTCAGGTTATAAAGT CAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAG ATTGATTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGG GACGTGCATGACATCGGCAAGAACATAGTTGGCGTGGTGCTGGCCTGTAACAATTTCAGAGTTATTGATT TAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTT GTCAGGACTCATCACTCCATCCTTGGATGAGATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATA AAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATTGCACCACGGT ACAGCGCGCCTGTGATCCACGTCCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGA AAATCTGAGAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTATGAG TCTCTCAAAGAGAGAAAATACGTACCCCTAAGTCAAGCCAGAAAACACGGTTTCCACATTGATTGGCTCT CTGAACCTCATCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAGGACTACAACCTGCAAAAGCT GGTGGACTACATTGACTGGAAGCCTTTCTTTGACGTCTGGCAGCTCCGGGGCAAGTACCCGAACCGAGGA TTTCCCAAGATATTTAATGACAAAGCAGTAGGTGAAGAAGCCAGAAAGGTATACAATGATGCTCAGAATA TGCTGAACATACTGATTAGTCAAAAGAAACTGCAGGCCAGGGGTGTGGTTGGATTCTGGCCAGCACAGAG TGTCCAGGATGACATCCACCTGTATGCAGAGGGGGTGGTGCCCCAGGCCGCTGAGCCCATAGCCACCTTC TATGGACTGAGGCAGCAGGCTGAGAAGGACTCTTCTAGTACAGACCCCTACCACTGCCTCTCAGACTTCA TTGCTCCTTTGCATTCTGGTGTCTGTGACTACTTGGGTCTGTTTGCTGTTGCCTGCTTTGGGGTTGAAGA GCTGAGTAAGACCTATGAGGATGATGGCGATGACTACAGCAGCATCATGGTGAAGGCGCTGGGCGACAGG CTGGCAGAGGCCTTTGCAGAGGAGCTCCATGAGAGAGTTCGCCGAGAACTGTGGGCCTACTCTAGGAGTG AGCAGTTGGGCGTCCCAGACTTGCGCAGACTCCGGTATGAGGGCATCCGGCCAGCTCCTGGTTACCCCAG CCAGCCTGACCATACTGAGAAGCTTACCATGTGGAGACTGGCCAGCATCGAGCAGGCCACAGGCATCAGG CTGACAGAATCCTTGGCAATGGCACCCGCTTCAGCAGTGTCAGGGCTGTACTTCTCCAACGTAAAGGCCA AATATTTTGCTGTGGGGAAAATTTCCAAAGATCAGACTGAGGATTATGCTTTGAGGAAGAACATGCCAGT GGCCGAGGTGGAGAAATGGCTTGGTCCCATTCTGGGCTATGACACAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218828 representing NM_001081128
Red=Cloning site Green=Tags(s) MKKTLQDEIEAILRKRIMVLDGGMGTMIQRYKLSEEHFQGQEFKDHSRPLKGNNDILSITQPDIIYQIHK EYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNKCSADVARKAAEEITLQTGVKRFVAGALGPTNKTL SVSPSVERPDYRNITFDELVDAYQEQAKGLLDGRVDILLIETIFDTANAKAALFAIQNLFEENYAPPRPI FISGTIVDKSGRTLSGQTGEAFVTSVSHSDPLCIGLNCSLGAAEMRPFIETIGKCTTAYVLCYPNAGLPN TFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEP FRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFC NSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDE EGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVR ISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEA TEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPL MNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKG DVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAI KIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYE SLKERKYVPLSQARKHGFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRG FPKIFNDKAVGEEARKVYNDAQNMLNILISQKKLQARGVVGFWPAQSVQDDIHLYAEGVVPQAAEPIATF YGLRQQAEKDSSSTDPYHCLSDFIAPLHSGVCDYLGLFAVACFGVEELSKTYEDDGDDYSSIMVKALGDR LAEAFAEELHERVRRELWAYSRSEQLGVPDLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLASIEQATGIR LTESLAMAPASAVSGLYFSNVKAKYFAVGKISKDQTEDYALRKNMPVAEVEKWLGPILGYDTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081128 |
| ORF Size | 3759 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081128.3, NP_001074597.1 |
| RefSeq Size | 4487 bp |
| RefSeq ORF | 3762 bp |
| Locus ID | 238505 |
| UniProt ID | A6H5Y3 |
| Gene Summary | Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224074 | Mtr (untagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), (10ug) |
CNY 16,060.00 |
|
| MR218828 | Mtr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr) |
CNY 8,728.00 |
|
| MR218828L3 | Lenti ORF clone of Mtr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr) |
CNY 12,830.00 |
|
| MR218828L4 | Lenti ORF clone of Mtr (mGFP-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr) |
CNY 12,830.00 |
