Ttc30b (NM_028235) Mouse Tagged ORF Clone
CAT#: MG218845
- TrueORF®
Ttc30b (tGFP-tagged) - Mouse tetratricopeptide repeat domain 30B (Ttc30b), (10ug)
ORF Plasmid: DDK
"NM_028235" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2510042P03Rik; Tcc30b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218845 representing NM_028235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGCTGAGCAGCTCACAGATCCCCGACGGTGAGTTCACAGCAGTGGTGTACAGGCTCATCCGCG ACTCCCGCTACTCAGAGGCGGTGCAGCTGCTGAGCGCAGAGCTGCAGAGGAGCTCCCGCAGCCGCGCAGG GCTGTCGCTGCTGGCCTACTGCTACTACCGCCTGCAGGAGTTCGAGCTCGCTGCAGAGTGCTATGAGCAG CTGAGCCAGATGCACCCGGAACTCGAGCAGTACCGCCTGTACCAGGCCCAGGCACTGTACAAGGCCTGCC TGTATCCAGAGGCCACCAGGGTCGCCTTCCTCCTGGACAACCCCGCCTATCAGACTCGAGTCCTTCGTCT CCAGGCTGCTATCAAGTACAGTGAAGGTGACCTCCCAGGAGCCAGGAGCCTGGTGGAGCAGCTGCTGAGT GGGGAAGGTGTAGAGGACAGTGGAGGGGAGAGTGATTATGATGGTCAGATCAACCTAGGTTGTCTGCTCT ACAAAGAGGGACACTATGAAGCTGCCTGTTCCAAGTTCTTAGCGGCCCTGCAGGCTTCTGGCTACCAGCC TGACCTTTCCTACAACTTGGCTTTGGCCTATTACAGCAGTCGGCAGTACGCGCCTGCTCTGAAGCATATC GCTGACATCATTGAGCGTGGCATCCGTCAACACCCAGAACTCGGTGTGGGCATGACCACGGAGGGCATTG ATGTTCGCAGTGTTGGCAACACTATAGTCCTTCACCAGACTGCTCTGGTCGAAGCCTTCAACCTCAAGGC AGCCATAGAGTACCAGCTGAGAAACTACGAGGCAGCCCAGGAAGCCCTCACTGACATGCCACCCAGAGCA GAGGAAGAGCTGGACCCCGTAACCCTCCACAACCAGGCTCTGATGAACATGGATGCCAAGCCCACAGAGG GCTTTGAAAAGCTCCAGTTTCTGCTCCAGCAGAACCCCTTCCCCCCAGAGACCTTTGGCAACCTGCTGCT GCTCTACTGTAAATATGAGTATTTTGACCTGGCAGCTGATGTCCTGGCAGAAAATGCCCACTTGACTTAC AAGTTCCTCACACCCTATCTCTATGACTTCTTGGATGCCATGATCACTTGCCAGACAGCTCCTGAAGAGG CTTTCATTAAACTTGATGGGTTGGCTGGCATGCTGACTGAGCAGCTCCGGAAACTCACCATACAAGTACA GGATTCAAGGCATAGTAGAGATGATGAATCTGCCAAAAAGGCTGTGAATGAATATGATGAAACTCTTGAG AAGTATATTCCTGTACTGATGGCCCAGGCAAAAATCTACTGGAACTTTGAGAATTACCCAATGGTGGAGA AGATCTTCCGCAAATCTGTGGAATTCTGTAATGACCATGATGTGTGGAAGCTGAATGTGGCCCATGTCCT CTTCATGCAGGAGAACAAGTACAAAGAGGCCATTGGTTTCTATGAGCCCATTGTCAAGAAGAATTATGAT AACATCCTGAGTGTCAGTGCTATTGTGTTAGCCAACCTCTGTGTTTCCTATATCATGACAAGTCAAAATG AGGAAGCTGAGGAGCTCATGAGGAAGATTGAGAAGGAGGAGGAACAACTCTCTTATGGTGACCCAGACAA GAAAATCTACCACCTTTGCATTGTGAATTTGGTGATAGGAACTCTGTATTGTGCCAAAGGAAACTATGAC TTTGGCATCTCTCGGGTTATCAAAAGCCTGGAGCCTTACCATAAAAAGCTAGGCACCGATACGTGGTATT ATGCCAAGAGATGTTTCCTGTCCTTACTAGAGAACATGTCAAAGCACACAATTATGCTAAGGGACAGTGT CATTCAAGAATGTGTGCAGTTTCTAGAGCACTGTGAACTTTATGGTAGAAACATTCCAGCTGTTATAGAA CAGCCCTTGGAGGAAGAAAGAATGCACACTGGGAAGAATACAGTCACATATGAATCAAGAAAGTTGAGGG CTCTGATTTATGAGATAATTGGATGGAATGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218845 representing NM_028235
Red=Cloning site Green=Tags(s) MAGLSSSQIPDGEFTAVVYRLIRDSRYSEAVQLLSAELQRSSRSRAGLSLLAYCYYRLQEFELAAECYEQ LSQMHPELEQYRLYQAQALYKACLYPEATRVAFLLDNPAYQTRVLRLQAAIKYSEGDLPGARSLVEQLLS GEGVEDSGGESDYDGQINLGCLLYKEGHYEAACSKFLAALQASGYQPDLSYNLALAYYSSRQYAPALKHI ADIIERGIRQHPELGVGMTTEGIDVRSVGNTIVLHQTALVEAFNLKAAIEYQLRNYEAAQEALTDMPPRA EEELDPVTLHNQALMNMDAKPTEGFEKLQFLLQQNPFPPETFGNLLLLYCKYEYFDLAADVLAENAHLTY KFLTPYLYDFLDAMITCQTAPEEAFIKLDGLAGMLTEQLRKLTIQVQDSRHSRDDESAKKAVNEYDETLE KYIPVLMAQAKIYWNFENYPMVEKIFRKSVEFCNDHDVWKLNVAHVLFMQENKYKEAIGFYEPIVKKNYD NILSVSAIVLANLCVSYIMTSQNEEAEELMRKIEKEEEQLSYGDPDKKIYHLCIVNLVIGTLYCAKGNYD FGISRVIKSLEPYHKKLGTDTWYYAKRCFLSLLENMSKHTIMLRDSVIQECVQFLEHCELYGRNIPAVIE QPLEEERMHTGKNTVTYESRKLRALIYEIIGWNV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028235 |
ORF Size | 1992 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028235.1, NP_082511.1 |
RefSeq Size | 2634 bp |
RefSeq ORF | 1995 bp |
Locus ID | 72421 |
UniProt ID | Q9CY00 |
Gene Summary | Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220266 | Ttc30b (untagged) - Mouse tetratricopeptide repeat domain 30B (Ttc30b), (10ug) |
CNY 8,550.00 |
|
MR218845 | Ttc30b (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 30B (Ttc30b) |
CNY 4,944.00 |
|
MR218845L3 | Lenti ORF clone of Ttc30b (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 30B (Ttc30b) |
CNY 6,460.00 |
|
MR218845L4 | Lenti ORF clone of Ttc30b (mGFP-tagged) - Mouse tetratricopeptide repeat domain 30B (Ttc30b) |
CNY 6,460.00 |