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Otop1 (NM_172709) Mouse Tagged ORF Clone
CAT＃: MG219221
- TrueORF®
Otop1 (tGFP-tagged) - Mouse otopetrin 1 (Otop1), (10ug)
ORF Plasmid: DDK
"NM_172709" in other vectors (4)
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CNY 5,800.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A530025J20Rik; Otp1; tlt |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219221 representing NM_172709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGGGCCCGGGCGCGCCCTCTTCTCCCGCAGCATCCTCAGGCTCCTCGCGTGCCGCGCCGTCGG GGATCGCGGCATGCCCTCTGTCCCCACCTCCCCTAGCCCGAGGGTCTCCACAAGCTTCCGGTCCCCGGCG CGGCGCCAGCGTCCCGCAGAAGCTGGCGGAGACTCTGAGCAGCCAGTATGGACTGAACGTGTTCGTGGCG GGGCTGCTGTTCCTGCTGGCCTGGGCGGTGCACGCGACGGGTGTGGGCAAGAGCGACCTGCTGTGCGTTC TAACCGCGCTTATGCTGCTGCAGCTGCTCTGGATGCTGTGGTACGTGGGTCGCAGCTACATGCAGCGCCG CCTCATCCGCCCCAAGGACACGCACGCGGGTGCGCGCTGGCTTCGCGGAAGCATCACGTTGTTCGCGTTT ATCACTGTCGTCCTGGGATGCTTGAAAGTCGCATACTTCATTGGATTCTCGGAGTGCTTGTCAGCCACCG AGGGAGTTTTCCCAGTCACCCATGCAGTGCATACCCTATTGCAGGTGTATTTCCTCTGGGGCCATGCTAA GGATATCATCATGTCTTTCAAAACACTGGAAAGGTTTGGGGTGATCCATTCAGTGTTCACGAACCTCCTA CTGTGGGCCAACAGCGTCCTGAATGAATCAAAGCACCAGCTGAATGAGCACAAGGAACGGCTGATCACTC TGGGCTTTGGCAACATCACCATCGTTTTGGATGACCACACACCACAGTGTAACTGCACACCACCCGCCCT CTGCTCTGCCCTCTCCCATGGGATTTACTATCTGTACCCCTTCAACATTGAGTACCAGATCCTGGCCTCG ACCATGCTCTACGTGCTGTGGAAGAACATCGGGCGCAGAGTGGACAGCTCCCAGCACCAGAAGATGCAGT GCAGATTCGACGGGGTCCTAGTGGGCTCCGTGCTGGGCTTGACAGTGCTGGCTGCCACCATCGCCGTGGT TGTGGTGTACATGATCCACATCGGGCGCTCCAAATCCAAGAGCGAGTCGGCTCTCATCATGTTCTATTTG TACGCTATCACGGTGCTGCTGCTTATGGGGGCCGCAGGGCTAGTCGGAAGCTGGATTTACAGGGTGGATG AGAAGTCTCTGGATGAGTCAAAGAACCCAGCGCGCAAGCTGGATGTTGACCTGTTGGTGGCCACCGGCTC CGGCTCCTGGCTCCTCTCCTGGGGCTCCATTCTGGCCATCGCCTGTGCTGAGACTCGCCCACCGTACACC TGGTACAACCTGCCCTACTCGGTCCTGGTGATCGTGGAGAAGTATGTCCAAAACATTTTCATCATCGAGT CCGTGCACCTCGAGCCTGAGGGGGTCCCGGAGGATGTGCGCACTCTGCGTGTGGTCACCGTCTGCAGCAG CGAGGCTGCCGCACTGGCTGCATCCACTCTCGGGAGCCAGGGGATGGCCCAGGATGGGTCACCTGCTGTC AATGGAAATCTGTGTCTGCAGCAGAGGTGTGGGAAAGAGGACCAGGAGTCTGGCTGGGAAGGAGCTACGG GGACAACCCGATGTCTGGACTTCCTTCAGGGCGGCATGAAGAGGAGGCTTCTCAGAAACATCACGGCCTT TCTGTTTCTTTGCAACATCTCGCTTTGGATTCCCCCTGCCTTTGGCTGCCGTCCCGAGTATGACAACGGA TTGGAGGAAATTGTCTTTGGCTTTGAACCCTGGATAATTGTGGTCAACCTGGCCATGCCCTTTTCCATTT TCTACCGGATGCACGCAGCTGCTGCCCTCTTTGAGGTCTATTGTAAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219221 representing NM_172709
Red=Cloning site Green=Tags(s) MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASGPRRGASVPQKLAETLSSQYGLNVFVA GLLFLLAWAVHATGVGKSDLLCVLTALMLLQLLWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAF ITVVLGCLKVAYFIGFSECLSATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLL LWANSVLNESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQILAS TMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHIGRSKSKSESALIMFYL YAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLLVATGSGSWLLSWGSILAIACAETRPPYT WYNLPYSVLVIVEKYVQNIFIIESVHLEPEGVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAV NGNLCLQQRCGKEDQESGWEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNG LEEIVFGFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172709 |
| ORF Size | 1800 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172709.3, NP_766297.2 |
| RefSeq Size | 3171 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 21906 |
| UniProt ID | Q80VM9 |
| Gene Summary | Proton-selective channel that specifically transports protons into cells (PubMed:29371428). Proton channel activity is only weakly-sensitive to voltage (PubMed:29371428). Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes (PubMed:29371428). In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration (PubMed:12651873). Probably acts by maintaining the pH appropriate for formation of otoconia (PubMed:29371428). Regulates purinergic control of intracellular calcium in vestibular supporting cells (PubMed:17606897, PubMed:20554841). May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol (PubMed:29371428). Also involved in energy metabolism, by reducing adipose tissue inflammation and protecting from obesity-induced metabolic dysfunction (PubMed:24379350).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219521 | Otop1 (untagged) - Mouse otopetrin 1 (Otop1), (10ug) |
CNY 7,700.00 |
|
| MR219221 | Otop1 (Myc-DDK-tagged) - Mouse otopetrin 1 (Otop1) |
CNY 4,464.00 |
|
| MR219221L3 | Lenti ORF clone of Otop1 (Myc-DDK-tagged) - Mouse otopetrin 1 (Otop1) |
CNY 7,130.00 |
|
| MR219221L4 | Lenti ORF clone of Otop1 (mGFP-tagged) - Mouse otopetrin 1 (Otop1) |
CNY 7,130.00 |
