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Mefv (NM_001161790) Mouse Tagged ORF Clone
CAT＃: MG220043
- TrueORF®
Mefv (tGFP-tagged) - Mouse Mediterranean fever (Mefv) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001161790" in other vectors (4)
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CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FMF; pyrin; TRIM20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220043 representing NM_001161790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGACCCTTGGTGATCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGACTTTGAGAAGT TCAAGTTTAAGCTACAGAACACCAGCCTGGAGAAGGGCCACTCTAAGATCCCCCGGGGCCATATGCAGAT GGCCAGGCCAGTAAAGCTGGCCAGCCTTCTGATAACCTACTACGGGGAGGAGTATGCTGTAAGGCTGACC CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCGCAAAGCCACAGGCACAGAAC ATTTAATTGAAGAAAATAGAGTTGGTGGTTCTGTCCAGTCTTCTGTAGAGAATAAAGCCAAGAGTGTGAA GGTACCAGATGTCCCTGAAGGTGATGGGACACAGCAAAACAATGATGAATCAGACACCCTTCCATCCAGC CAGGCTGAAGTGGGGAAGGGACCCCAGAAGAAGTCACTGACCAAAAGGAAGGATCAGAGGGGCCCCGAGA GCCTGGACTCACAGACCAAGCCATGGACAAGGAGCACAGCGCCTCTCTATAGGAGAACCCAGGGCACCCA GTCCCCAGGGGATAAGGAGAGCACAGCAAGTGCCCAGCTCCGCAGGAATGTCAGCTCTGCAGGGAGGCTT CAAGGACTTTACAACAATGCCCCAGGGAGGAGAGAAAGCAAGAAAGCCGAAGTGTATGTATATTTGCCTT CAGGAAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTATTCAAGAGAAGGAGAACCCCCAAATTCAGA AGTTCTTCCAACTCAAGAGGAAACAAGAAATGGGAGTCTCATTCGCATGAGAACAGCTACTCTGAATGGA AGGACTACGGGGGCTTTAGAAAAGGGCACAGGGATTCCAGAGCATTCCATGGTGCTGGATGAAAAAACAT TCAGAAACATGTCTTCCAAAACATCGTTGATTGGGGAGGAGAGATGCCCTACATCATGGACGGAAAATGG AAATGGGAGTCCAGAAACCACAGAGTCCTCGGGAGAGACGGCTGGCAGCATACTCTCTGATCCAGAAGTG CCTTTGTCACTATGTGAAAAACCAGCTAAAACTCCAGAAGACCCAGCATCCTTAGGACAGGCCGCTTGTG AAGGTACCCTCCTTCAGGTCTCAAGAAAATGTGATGCAGGCCCAGAAAAATGCACATTTACACATCTTGC ACTACAGGTTGCATCAAATTTCGGAAGGTCACAGGACAAGGCTGTGTGCCCTCTTTGCCACACCCAGGAA GGAGACCTGCGTGGTGATACCTGTGTGCAAAGTTCCTGTAGCTGCTCCATTGCTCCTGGGGATCCCAAGG CCTCCGGCAGATGCTCCATATGCTTTCAGTGCCAAGGCTTACTTGCCAGAAAGAGCTGCGAAGCCCAGAG CCCCCAGTCCCTACCACAGTGCCCTCGTCACATGAAGCAGGTGCTGCTGCTTTTCTGTGAGGACCACAGG GAGCCCATCTGCCTCATCTGCAGGCTGAGCCTGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGG CTGCACTAGAGTACAAGGAGCAGATCCGGGAGCAGCTGGAGCGCCTGCGGGAGATGAGGGGATATGTGGA GGAGCACAGGCTACAGGGAGACAAGAAAACAGACGATTTCCTGAAACAAACAGAAATCCAGAAGCAGAAG ATTTCATGTCCACTTGAGAAGCTATACCAACTCCTGGAGAAGCAAGAACAACTCTTTGTGACCTGGCTAC AGGAGCTAAGCCAGACCATCAGCAAGGTCAGAGAGACATACTACACCCGGGTTTCCCTACTGGATGAGAT GATTGAAGAACTAGAGGCCAAGCAAGACCAGCCAGAGTGGGACCTCATGCAGGACATTGGAATCACCTTG CACAGGGCTAAGATGATGTCTGCCTCTGAGCTGTTGGACACTCCTCCAGGTGTTAAAGAAAAGCTTCATC TGCTCTACCAGAAGTCAAAGTCTGTGGAGAAGAATATGCAGTGTTTCTCAGAAATGCTGAGCTCTGAAAT GGCATTCAGCGCTTCAGATGTAGCAAAATGGGAAGGGCGCCAGCCTAGCGCCACACAGGTCCAGGGCTTG GTTCCCACCGTTCACCTAAAGTGTGATGGTGCACACACACAGGACTGTGATGTAGTATTTTACCCAGAAC GGGAAGCTGGAGGATCAGAACCTAAAGATTACCTTCATCCTCAGCCAGCTCAAGACACACCTGAGCTACA TGAGATCCATTCTCGGAACAACAAAAGAAAATTTAAATCTTTCCTGAAATGGAAACCTTCATTCATTGGG AGCACCTTGGGAGAAGCCCACAGATCCGCACTCCCAGCTAGGCATTCTTCTTCCTGCAGTTACCGTGATT TGGACCAAGCAGCTGCTCACCCCAACCTCATCTTCTCGATGATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220043 representing NM_001161790
Red=Cloning site Green=Tags(s) MAKTLGDHLLNTLEELLPYDFEKFKFKLQNTSLEKGHSKIPRGHMQMARPVKLASLLITYYGEEYAVRLT LQILRATNQRQLAEELRKATGTEHLIEENRVGGSVQSSVENKAKSVKVPDVPEGDGTQQNNDESDTLPSS QAEVGKGPQKKSLTKRKDQRGPESLDSQTKPWTRSTAPLYRRTQGTQSPGDKESTASAQLRRNVSSAGRL QGLYNNAPGRRESKKAEVYVYLPSGKKRPRSLEITTYSREGEPPNSEVLPTQEETRNGSLIRMRTATLNG RTTGALEKGTGIPEHSMVLDEKTFRNMSSKTSLIGEERCPTSWTENGNGSPETTESSGETAGSILSDPEV PLSLCEKPAKTPEDPASLGQAACEGTLLQVSRKCDAGPEKCTFTHLALQVASNFGRSQDKAVCPLCHTQE GDLRGDTCVQSSCSCSIAPGDPKASGRCSICFQCQGLLARKSCEAQSPQSLPQCPRHMKQVLLLFCEDHR EPICLICRLSLEHQGHRVRPIEEAALEYKEQIREQLERLREMRGYVEEHRLQGDKKTDDFLKQTEIQKQK ISCPLEKLYQLLEKQEQLFVTWLQELSQTISKVRETYYTRVSLLDEMIEELEAKQDQPEWDLMQDIGITL HRAKMMSASELLDTPPGVKEKLHLLYQKSKSVEKNMQCFSEMLSSEMAFSASDVAKWEGRQPSATQVQGL VPTVHLKCDGAHTQDCDVVFYPEREAGGSEPKDYLHPQPAQDTPELHEIHSRNNKRKFKSFLKWKPSFIG STLGEAHRSALPARHSSSCSYRDLDQAAAHPNLIFSMI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161790 |
| ORF Size | 2424 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161790.1, NP_001155262.1 |
| RefSeq Size | 3249 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 54483 |
| Gene Summary | Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221759 | Mefv (untagged) - Mouse Mediterranean fever (Mefv), transcript variant 1, (10ug) |
CNY 9,480.00 |
|
| MR220043 | Mefv (Myc-DDK-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 1 |
CNY 9,464.00 |
|
| MR220043L3 | Lenti ORF clone of Mefv (Myc-DDK-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 1 |
CNY 9,980.00 |
|
| MR220043L4 | Lenti ORF clone of Mefv (mGFP-tagged) - Mouse Mediterranean fever (Mefv), transcript variant 1 |
CNY 9,980.00 |
