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Foxe1 (NM_183298) Mouse Tagged ORF Clone
CAT＃: MG220588
- TrueORF®
Foxe1 (tGFP-tagged) - Mouse forkhead box E1 (Foxe1), (10ug)
ORF Plasmid: DDK
"NM_183298" in other vectors (2)
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CNY 3,140.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Titf2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220588 representing NM_183298
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCCGAGAGCGCGCCGCCTCCCCCGCCGCAGCCCGAGACGCTGGCGGCCGTGAAGGAAGAGCGCG GTGAGGCGGCGGCGGCGGGCGCGGGGGTCCCGGCGGAGGCTGCGGGTCGCGGCGCGGGAGGACGGCGACG CAAGCGCCCCCTGCAGCGGGGGAAGCCGCCCTACAGCTACATCGCGCTCATCGCTATGGCCATCGCGCAC GCGCCCGAGCGCCGCCTGACTCTGGGCGGCATCTACAAGTTCATCACCGAGCGCTTCCCGTTCTACCGCG ACAACCCCAAGAAGTGGCAGAACAGCATCCGCCACAACCTCACCCTCAACGACTGCTTCCTCAAGATCCC GCGCGAGGCGGGCCGCCCGGGCAAGGGCAATTACTGGGCGCTCGACCCCAACGCCGAGGACATGTTCGAA AGCGGCAGCTTCCTGCGCCGCCGCAAGCGCTTCAAGCGCTCGGACCTGTCCACCTACCCGGCCTACATGC ACGATGCAGCCGCTGCCGCCGCCGCTGCCGCTGCCGCCATCTTCCCGGGCGCCGTGCCTGCCGCAAGGCC GGCTTACCCGGGCGCCGTCTATGCGGGCTACGCGCCGCCACTCGCCGCGCCTCCGCCGGTCTACTACCCC GCCGCGTCGCCGGGGCCCTGCCGGGTCTTCGGCTTGGTTCCCGAGCGGCCGCTCAGCCCCGATCTGGGCC CCGCGCCGTCAGCGGCCGGCGGCTCGTGCGCCTTCGCGGCTGCGGCGGGCGCGGCGGGCACCGGGAGCTT CCAGCCGGCGGTGTGCACCGGAGCGCGGCCGGTCAACCCCGCCGCCTATGCCGCCGCCTACGCGGGCCCC GACGGCGCGTACCCGCAAGGAGCGAGCAGTGCGCTCTTCGCCGCTGCTGCGGGCCGCCTGGCCGGACCGG CCTCGCCCCCCGCGGGAGGCGGCAGCGGCGGCGTGGAGGCCACGGTGGACTTCTACGGGCGCACGTCGCC GGGCCAGTTCGGAGCGGCGCTGGGACCCTGCTACAATCCCGGTGGGCAACTCGGAGCCGGTGGTGGCGGT GCCTACCACTCCCGCCACGCCACCGCCTACCCCGGCGCGGTGGATCGGTTCGTGTCTGCCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220588 representing NM_183298
Red=Cloning site Green=Tags(s) MTAESAPPPPPQPETLAAVKEERGEAAAAGAGVPAEAAGRGAGGRRRKRPLQRGKPPYSYIALIAMAIAH APERRLTLGGIYKFITERFPFYRDNPKKWQNSIRHNLTLNDCFLKIPREAGRPGKGNYWALDPNAEDMFE SGSFLRRRKRFKRSDLSTYPAYMHDAAAAAAAAAAAIFPGAVPAARPAYPGAVYAGYAPPLAAPPPVYYP AASPGPCRVFGLVPERPLSPDLGPAPSAAGGSCAFAAAAGAAGTGSFQPAVCTGARPVNPAAYAAAYAGP DGAYPQGASSALFAAAAGRLAGPASPPAGGGSGGVEATVDFYGRTSPGQFGAALGPCYNPGGQLGAGGGG AYHSRHATAYPGAVDRFVSAM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183298 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183298.2 |
| RefSeq Size | 1116 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 110805 |
| UniProt ID | Q8R2I0 |
| Gene Summary | Transcription factor that binds consensus sites on a variety of gene promoters and activate their transcription. Involved in proper palate formation, most probably through the expression of MSX1 and TGFB3 genes which are direct targets of this transcription factor (PubMed:21177256). Also implicated in thyroid gland morphogenesis (PubMed:9697704). May indirectly play a role in cell growth and migration through the regulation of WNT5A expression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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