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Atp2b1 (NM_026482) Mouse Tagged ORF Clone
CAT＃: MG221085
- TrueORF®
Atp2b1 (tGFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 1 (Atp2b1), (10ug)
ORF Plasmid: DDK
"NM_026482" in other vectors (4)
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CNY 10,096.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810442I22Rik; E130111D10Rik; Pmca1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221085 representing NM_026482
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACATGGCCAACAACTCAGTTGCGTATAGCGGTGTGAAGAACTCTTTGAAAGAAGCTAACCATG ATGGAGACTTTGGAATTACGCTTACAGAGCTGCGAGCTCTCATGGAGCTCAGATCCACGGATGCATTACG GAAAATACAGGAGAGCTATGGAGATGTCTATGGCATTTGCACCAAGTTGAAAACATCTCCCAATGAAGGT TTAAGTGGAAATCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAACTTTATACCTCCTAAGA AGCCAAAAACCTTTCTTCAGTTAGTCTGGGAAGCATTACAAGATGTCACTTTAATTATACTAGAAATTGC AGCCATAGTATCATTGGGCCTTTCTTTTTATCAACCTCCGGAAGGGGATAATGCACTTTGTGGAGAAGTT TCTGTTGGGGAAGAAGAAGGTGAAGGAGAAACGGGGTGGATTGAAGGAGCTGCGATCCTCTTGTCGGTGG TGTGTGTGGTGTTGGTGACGGCTTTCAACGACTGGAGCAAGGAGAAGCAGTTCCGAGGCCTGCAGAGTCG AATTGAGCAGGAACAGAAGTTCACCGTCATCAGGGGTGGGCAGGTCATCCAGATACCTGTAGCTGACATT ACTGTTGGAGACATTGCCCAAGTGAAGTACGGTGACCTTCTTCCAGCTGATGGCATACTGATTCAAGGCA ATGACCTGAAGATTGATGAGAGCTCATTGACTGGTGAATCTGATCATGTTAAGAAGTCTCTAGATAAGGA TCCCTTACTTCTTTCAGGTACTCATGTGATGGAAGGTTCTGGAAGAATGGTGGTCACTGCTGTAGGTGTG AATTCTCAAACAGGAATCATCTTCACCTTACTTGGAGCTGGGGGTGAAGAAGAGGAAAAGAAAGATGAGA AGAAGAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAAGCAAAAGCCCAGGA CGGCGCGGCCATGGAGATGCAACCTCTGAAGAGTGAAGAAGGAGGGGACGGTGATGAGAAAGACAAGAAG AAAGCAAATCTGCCAAAAAAGGAAAAATCCGTGTTACAGGGGAAACTCACAAAGCTGGCTGTTCAGATTG GCAAAGCAGGTCTGCTGATGTCTGCCATCACAGTTATCATCCTAGTGTTGTATTTTGTGATCGATACCTT CTGGGTTCAGAAAAGACCATGGCTTGCAGAGTGCACACCGATCTACATCCAGTACTTCGTGAAGTTCTTC ATTATTGGAGTCACAGTTCTAGTGGTCGCCGTGCCGGAAGGTCTTCCGCTGGCAGTCACCATCTCACTGG CTTACTCAGTCAAGAAAATGATGAAGGATAATAACTTAGTGAGACATCTGGATGCTTGTGAGACCATGGG GAATGCTACAGCTATCTGTTCAGATAAAACAGGAACCCTGACGATGAACAGGATGACGGTTGTTCAAGCT TACATAAATGAAAAGCATTATAAAAAGGTTCCGGAACCAGAAGCCATCCCACCAAATATCCTGTCCTACC TGGTAACGGGGATTTCTGTGAATTGTGCTTATACGTCAAAAATATTGCCACCGGAGAAAGAGGGTGGATT ACCCCGCCATGTGGGTAACAAAACAGAGTGTGCCTTGTTGGGGTTTCTCTTGGATTTAAAACGGGATTAT CAAGATGTTAGAAATGAAATACCAGAGGAAGCACTGTACAAAGTCTATACCTTCAATTCTGTTAGGAAGT CCATGAGCACAGTCCTGAAAAACTCAGATGGAAGTTTCCGCATCTTTAGCAAGGGTGCCTCCGAGATCAT TCTGAAAAAGTGTTTCAAAATCCTGAGTGCTAATGGCGAGGCAAAAGTATTCCGACCCAGAGACCGCGAT GACATTGTGAAAACTGTGATCGAGCCCATGGCATCAGAAGGCCTGAGGACCATATGCCTAGCGTTCAGAG ACTTCCCAGCGGGGGAGCCAGAACCAGAGTGGGATAATGAGAATGATGTCGTCACCGGCCTTACGTGTAT CGCGGTTGTGGGGATTGAAGATCCTGTGAGGCCTGAGGTACCAGAGGCAATAAAGAAATGTCAGAGGGCT GGAATTACTGTGCGCATGGTCACTGGTGATAATATTAACACTGCTCGGGCCATTGCTACCAAATGTGGTA TTTTACACCCAGGGGAAGATTTCCTATGCTTAGAAGGTAAAGATTTTAACCGAAGAATACGAAATGAAAA AGGAGAGATTGAACAAGAAAGGATAGACAAGATTTGGCCAAAGCTTCGAGTACTTGCAAGATCATCTCCC ACTGACAAACATACACTGGTGAAAGGTATAATTGACAGCACAGTCTCAGAGCAACGACAGGTTGTAGCTG TAACGGGTGATGGTACAAATGACGGGCCTGCACTGAAGAAAGCAGACGTTGGCTTTGCAATGGGCATTGC TGGAACTGATGTGGCTAAAGAAGCGTCTGATATTATTCTCACAGATGACAACTTTACAAGCATTGTCAAA GCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAGCTTACTGTTAATG TAGTAGCAGTGATTGTTGCTTTCACGGGCGCTTGTATTACTCAAGACTCGCCACTTAAGGCGGTGCAGAT GCTCTGGGTAAACCTCATCATGGACACGCTGGCTTCCCTGGCTCTGGCTACGGAACCACCCACCGAGTCA CTCCTGCTTCGGAAGCCTTATGGTAGAAATAAGCCTCTCATCTCACGCACGATGATGAAGAACATCTTGG GCCATGCATTCTATCAGCTCGTAGTGGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGATATCGA CAGCGGGAGGAATGCGCCTCTGCACGCTCCCCCGTCAGAACATTACACCATCGTGTTCAATACCTTTGTG CTGATGCAGCTCTTCAATGAAATAAACGCCCGGAAAATTCACGGGGAAAGAAATGTGTTTGAAGGAATCT TCAATAACGCCATCTTCTGCACCATTGTCCTGGGCACCTTTGTGGTGCAGATAATAATTGTGCAGTTTGG CGGGAAGCCTTTCAGCTGCTCAGAACTTTCAATAGAGCAATGGCTGTGGTCGATATTCCTGGGAATGGGG ACCTTACTCTGGGGCCAGCTTATTTCAACCATTCCAACCAGCCGCTTAAAGTTTCTAAAAGAAGCTGGTC ATGGAACCCAGAAGGAGGAGATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGACCATGCCGA AAGGGAGTTGCGGCGTGGCCAGATCTTGTGGTTTAGAGGCCTGAACAGAATCCAAACACAGATCCGAGTG GTGAATGCATTTCGTAGTTCTTTATACGAAGGGTTAGAGAAGCCAGAATCAAGAAGTTCAATCCACAACT TTATGACACACCCTGAGTTTAGGATAGAAGATTCAGAGCCGCACATCCCCCTTATTGATGACACTGATGC TGAAGACGACGCCCCAACAAAACGCAACTCCAGCCCTCCGCCCTCTCCTAACAAAAATAACAATGCTGTT GACAGCGGAATTCACCTTACAATAGAAATGAACAAGTCTGCTACCTCTTCATCCCCAGGAAGCCCACTAC ATAGTTTGGAAACATCACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221085 representing NM_026482
Red=Cloning site Green=Tags(s) MGDMANNSVAYSGVKNSLKEANHDGDFGITLTELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEG LSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEV SVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADI TVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGV NSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQA YINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGFLLDLKRDY QDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSFRIFSKGASEIILKKCFKILSANGEAKVFRPRDRD DIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDVVTGLTCIAVVGIEDPVRPEVPEAIKKCQRA GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSP TDKHTLVKGIIDSTVSEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTES LLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFV LMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRV VNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAV DSGIHLTIEMNKSATSSSPGSPLHSLETSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026482 |
| ORF Size | 3660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026482.2, NP_080758.1 |
| RefSeq Size | 7130 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 67972 |
| UniProt ID | G5E829 |
| Gene Summary | Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed:22311909, PubMed:16956963, PubMed:28827723, PubMed:26392310, PubMed:29950683, PubMed:24805951, PubMed:23266958). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction (PubMed:24805951, PubMed:29950683, PubMed:22311909). Positively regulates bone mineralization through absorption of calcium from the intestine (PubMed:23266958, PubMed:26392310). Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (PubMed:23266958). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223998 | Atp2b1 (untagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1), (10ug) |
CNY 15,680.00 |
|
| MR221085 | Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1) |
CNY 8,496.00 |
|
| MR221085L3 | Lenti ORF clone of Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1) |
CNY 14,060.00 |
|
| MR221085L4 | Lenti ORF clone of Atp2b1 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1) |
CNY 14,060.00 |
