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Atp2b1 (NM_026482) Mouse Tagged Lenti ORF Clone
CAT＃: MR221085L4
- LentiORF®
Lenti ORF clone of Atp2b1 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1)
"NM_026482" in other vectors (4)
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CNY 14,060.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 2810442I22Rik; E130111D10Rik; Pmca1 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR221085).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026482 |
| ORF Size | 3660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026482.2, NP_080758.1 |
| RefSeq Size | 7130 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 67972 |
| UniProt ID | G5E829 |
| Gene Summary | Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed:22311909, PubMed:16956963, PubMed:28827723, PubMed:26392310, PubMed:29950683, PubMed:24805951, PubMed:23266958). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction (PubMed:24805951, PubMed:29950683, PubMed:22311909). Positively regulates bone mineralization through absorption of calcium from the intestine (PubMed:23266958, PubMed:26392310). Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (PubMed:23266958). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223998 | Atp2b1 (untagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1), (10ug) |
CNY 15,680.00 |
|
| MG221085 | Atp2b1 (tGFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 1 (Atp2b1), (10ug) |
CNY 10,096.00 |
|
| MR221085 | Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1) |
CNY 8,496.00 |
|
| MR221085L3 | Lenti ORF clone of Atp2b1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 1 (Atp2b1) |
CNY 14,060.00 |
