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Kdm4a (NM_001161823) Mouse Tagged ORF Clone
CAT＃: MG221564
- TrueORF®
Kdm4a (tGFP-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001161823" in other vectors (4)
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CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D4Ertd222e; JHDM3A; Jmjd2; Jmjd2a; mKIAA0677 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221564 representing NM_001161823
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTGAATCAGAAACTTTGAACCCCAGTGCTCGGATCATGACCTTTTACCCCACCATGGAAGAGT TCCGGAACTTCAGCCGCTACATTGCCTACATTGAATCCCAAGGAGCTCATCGGGCTGGGCTAGCCAAGGT CGTTCCTCCCAAAGAGTGGAAGCCTCGAACATCCTACGACGATATTGATGATCTAGTCATCCCTGCGCCC ATCCAGCAGCTGGTGACTGGCCAGTCTGGCCTCTTCACTCAGTACAACATACAGAAGAAAGCCATGACCG TTCGTGAGTTCCGCAAGATCGCCAATAGCGACAAGTACTGCACCCCGCGATACAGTGAATTTGAAGAACT TGAGCGTAAATACTGGAAAAACCTCACATTCAATCCTCCCATCTATGGAGCAGACGTGAATGGTACTCTC TATGAACAGCACGTTGATGAGTGGAATATCGGCCGGCTGAAGACCATCCTGGACCTGGTGGAGAAGGAGA GCGGGATCACCATTGAGGGGGTGAACACCCCCTACCTTTACTTCGGCATGTGGAAGACGTCCTTCGCCTG GCACACGGAAGACATGGATCTATACAGCATCAACTATCTGCACTTCGGAGAGCCAAAGTCTTGGTACTCT GTTCCACCTGAGCATGGGAAACGCCTGGAGCGTCTTGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCT GTGAGGCTTTTCTCCGCCACAAGATGACCCTGATCTCCCCATTAATGCTGAAGAAGTATGGCATTCCCTT TGACAAGGTGACCCAAGAAGCTGGCGAGTTTATGATCACTTTTCCGTATGGTTACCATGCTGGCTTCAAC CATGGCTTCAACTGTGCAGAGTCAACCAATTTTGCTACCCGTCGGTGGATTGAGTATGGCAAGCAGGCTG TGCTGTGCTCCTGCAGAAAAGATATGGTGAAGATCTCCATGGACGTGTTCGTGAGGAGGTTCCAGCCGGA ACGCTACAAACTTTGGAAAGCTGGGAAGGACAGCATGGTTATTGACCACACTCTGCCCACACCGGAAGCA GCCGAGTTCCTGAAGGACAGCGGCGGACTAACCCCGAGAGCAGGGAGTGAGGAGTGCCCAGAGGAGGACG TGGAAGCAGCGGATCAGGGAGAGGAGGGGGATGTGAAGAGAAGCCTGGCCAAGCATCGGATCGGGACAAA GAGACACAGAGTCTGTCTGGAAATACCCCAGGAGGTCAGTCAGAGCGAGCTCTTCCCCAAGGAAGAGCTA AGTTCTGGACAGTATGAGATGACAGAGTGCCCGGCCACCCTCGCCCCAGTGAGGCCCACCCACAGCTCCG TGCGGCAAGTTGAGGACAGTCTTCCCTTCCCAGATTACTCTGACCCCACTGAAGTCAAATTTGAAGAGCT GAAGAATGTCAAACTAGAAGAGGAAGATGAGGAGGATGAGCCCGAAGCAGCCGCACTGGACCTTTCTGTG AATCCTGCGTCTGTAGGAGGACGCCTCGTCTTCTCGGGTTCCAAAAAGAAATCATCTTCCAGCCTGGGCT CCACTTCATCTCAGGATTCAGTTTCTTCAGATTCTGAAACCGCCGAGTCTGTCTCTTGCCAGGGCCAAGA GAAAACGGGAGTTCTCACCGTGCACAGCTATGCCAGAGGGGACGGCAAGGCTGCCACAGGAGAGCCGAGT GTGAAGAAGAAGCGCAGTGCTCCCCGCAGCATCAGCGAGCAGGAGCTGGCAGAGGTTGCAGATGAGTACA TGCTTTCCCTGGAGGAGAATAAGAAGACCAAGGGTCGCCGCCAGCCCTTGAGCAAGCTCCCACGCCACCA CCCACTCGTGCTGCAGGAGTGCGGCAGCGATGACGAGACTTCTGAGCAGCTGACCCCCGAGGAGGAGGCT GAGGAGACAGAGGCTTGGGCCAAGCCTCTGAGCCAGCTGTGGCAGAACCGACCCCCGAACTTTGAGGCTG AAAAGGAATTCAATGAGATCATGGCCCAACAGGCCCCTCACTGTGCTGTGTGTATGATCTTCCAGACCTA CCATCAGGTGGAATTTGGAGCCTTCAGTCAGAGCTGTGGGGATGCTTCAGAGCCGGCTGCCCAGACTCAG AGGACCAAGCCACTGATCCCCGAAATGTGCTTTACCACCACTGGCTGCAGCACAGACATCAATCTGTCCA CCCCGTACCTAGAGGAGGATGGCACCAGCATGCTAGTGTCCTGCAAGAAGTGCAGTGTCCGGGTCCACGC CAGTTGCTATGGCGTCCCTCCTGCCAAGGCTTCTGAAGAATGGATGTGCTCTCGATGTTCAGCCAATGCC CTGGAGGAAGACTGCTGCTTATGCTCGTTGCGTGGAGGGGCCCTGCAGAGAGCCAATGACGACAGATGGG TTCATGTTTCATGTGCAGTGGCAATTCTAGAAGCAAGGTTTGTCAACATTGCTGAAAGAAGTCCAGTGGA TGTGAGCAAAATCCCTCTGCCCCGCTTCAAACTGAAGTGTGTTTTTTGTAAGAAGCGGAGAAAACGGAAT GCTGGCTGCTGTGTGCAGTGCTCCCATGGCCGCTGCCCCACTGCCTTCCATGTGAGCTGTGCCCAGGCTG CTGGGGTGATGATGCAACCCGACGACTGGCCCTTTGTTGTCTTCATCACGTGCTTCCGTCACAAGATTCC CAATCTGGAGCGTGCTAAGGGAGCCTTGCTGAGCATCACGGCGGGCCAGAAGGTTATCAGCAAACACAAG AACGGGCGCTTCTACCAGTGTGAGGTGGTCAGACTCACCACCGAGACCTTCTACGAAGTCAACTTTGACG ACGGCTCCTTCAGTGACAACCTTTATCCTGAGGACATAGTGAGTCAGGACTGTCTCCAACTGGGGCCTCC TGCTGAAGGTGAAGTGGTTCAAGTCAGATGGACAGATGGCCAAGTCTATGGGGCTAAGTTTGTGGCCTCC CACCCCATCCAGATGTACCAGGTGGAGTTTGAGGATGGCTCGCAGCTGGTGGTTAAGAGAGATGATGTAT ACACACTCGATGAAGAGCTTCCCAAGAGAGTCAAGTCTAGACTGTCGGTGGCTTCAGACATGCGCTTCAA TGAGATTTTCACAGAGAAGGAAGTGAAGCAAGAAAAGAAGAGGCAACGGGTCATCAACTCGCGGTACCGG GAGGATTACATCGAGCCAGCGCTGTACCGGGCCATCATGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221564 representing NM_001161823
Red=Cloning site Green=Tags(s) MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRTSYDDIDDLVIPAP IQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTL YEQHVDEWNIGRLKTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYS VPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFN HGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRRFQPERYKLWKAGKDSMVIDHTLPTPEA AEFLKDSGGLTPRAGSEECPEEDVEAADQGEEGDVKRSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEEL SSGQYEMTECPATLAPVRPTHSSVRQVEDSLPFPDYSDPTEVKFEELKNVKLEEEDEEDEPEAAALDLSV NPASVGGRLVFSGSKKKSSSSLGSTSSQDSVSSDSETAESVSCQGQEKTGVLTVHSYARGDGKAATGEPS VKKKRSAPRSISEQELAEVADEYMLSLEENKKTKGRRQPLSKLPRHHPLVLQECGSDDETSEQLTPEEEA EETEAWAKPLSQLWQNRPPNFEAEKEFNEIMAQQAPHCAVCMIFQTYHQVEFGAFSQSCGDASEPAAQTQ RTKPLIPEMCFTTTGCSTDINLSTPYLEEDGTSMLVSCKKCSVRVHASCYGVPPAKASEEWMCSRCSANA LEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCVFCKKRRKRN AGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALLSITAGQKVISKHK NGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQLGPPAEGEVVQVRWTDGQVYGAKFVAS HPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYR EDYIEPALYRAIME TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161823 |
| ORF Size | 3192 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161823.1, NP_001155295.1 |
| RefSeq Size | 4616 bp |
| RefSeq ORF | 3195 bp |
| Locus ID | 230674 |
| UniProt ID | Q8BW72 |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:24953653). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223429 | Kdm4a (untagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1, (10ug) |
CNY 13,680.00 |
|
| MR221564 | Kdm4a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1 |
CNY 11,112.00 |
|
| MR221564L3 | Lenti ORF clone of Kdm4a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1 |
CNY 13,512.00 |
|
| MR221564L4 | Lenti ORF clone of Kdm4a (mGFP-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 1 |
CNY 12,540.00 |
