Ltk (NM_203345) Mouse Tagged ORF Clone
CAT#: MG222629
- TrueORF®
Ltk (tGFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk) transcript variant 4, (10ug)
ORF Plasmid: DDK
"NM_203345" in other vectors (4)
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CNY 8,550.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222629 representing NM_203345
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCTCCCACCGGCTGCTGCTGTGGCTGGGAGCTGCTGGAACTATTCTTTGCTCCAACTCGGAGT TCCAGACACCTTTTCTAACACCTTCGCTTTTGCCGGTGCTGGTACTCAATTCCCAGGAGCAGAAAGTCAC CCCCACACCCAGTAAATTGGAGCCAGCTTCCCTCCCAAATCCTCTAGGCACACGGGGGCCTTGGGTGTTT AACACCTGTGGCGCCAGCGGCAGGAGCGGACCCACACAAACACAGTGCGATGGGGCATACACAGGAAGCA GCGTGATGGTGACAGTGGGGGCTGCCGGGCCGCTCAAAGGCGTGCAGCTGTGGCGGGTGCCAGACACAGG CCAGTATCTGATCTCCGCCTACGGAGCGGCGGGCGGCAAGGGCGCCCAAAACCACCTGTCACGGGCGCAC GGCATCTTCCTCTCAGCAGTCTTCTTCCTCCGTCGCGGGGAGCCGGTGTACATCCTTGTGGGGCAGCAGG GCCAGGACGCCTGTCCCGGAGGGAGCCCTGAGAGCCAACTCGTCTGTCTGGGAGAGTCTGGGGAGCATGC AACCACCTATGGGACCGAAAGGATCCCAGGCTGGAGACGCTGGGCCGGCGGGGGCGGGGGTGGCGGAGGC GCCACCTCCATCTTCCGGCTGCGCGCGGGGGAGCCAGAGCCGCTGCTGGTGGCGGCGGGAGGCGGCGGGA GGTCCTACCGGAGGCGACCTGACCGCGGCCGGACTCAGGCCGTCCCCGAGAGGCTGGAGACCCGCGCGGC GGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGTGGAGGGAGCGGCTGGACGTCGCGAGCCCACTCT CCGCAGGCCGGACGCTCGCCGCGGGAAGGGGCCGAGGGCGGCGAGGGCTGCGCGGAGGCCTGGGCTGCGC TCCGCTGGGCTGCGGCGGGAGGCTTCGGGGGCGGCGGCGGGGCCTGCGCGGCGGGCGGCGGTGGCGGCGG CTACCGGGGCGGTGATACTTCTGAGTCTGACCTCCTCTGGGCTGATGGGGAAGATGGCACATCCTTTGTC CACCCCAGTGGTGAGCTCTACCTACAGCCTCTGGCAGTCACAGAGGGCCATGGGGAGGTGGAGATCCGAA AGCATCCCAACTGCAGTCACTGCCCTTTCAAAGACTGCCAGTGGCAGGCAGAGCTCTGGACGGCCGAATG CACGTGCCCAGAGGGCACGGAGCTAGCTGTGGATAATGTCACTTGCATGGACCTGCCAACCACCGCAAGC CCTCTGATCCTGATGGGAGCTGTAGTGGCAGCCTTGGCACTGAGTCTCCTAATGATGTGTGCAGTCCTGA TTCTAGTGAACCAGAAGTGTCAGGGCCTGTGGGGGACCAGGCTGCCAGGCCCTGAGCTTGAGCTAAGCAA GCTTCGATCCTCTGCCATCAGGACAGCACCCAACCCTTACTATTGTCAGGTGGGACTCAGTCCTGCCCAG CCCTGGCCTTTGCCCCCAGGGCTCACTGAGGTTTCACCAGCCAATGTCACTCTACTCAGAGCCCTTGGCC ATGGTGCCTTTGGGGAAGTGTACGAGGGACTAGTGACTGGTCTTCCTGGGGACTCCAGTCCTCTTCCAGT GGCTATTAAGACTCTGCCAGAGCTCTGCTCCCATCAGGATGAGCTGGATTTTCTCATGGAGGCTCTGATC ATCAGCAAGTTCAGCCATCAGAACATTGTACGCTGTGTGGGGCTCAGCTTTCGGTCTGCCCCGCGCCTCA TTCTGCTGGAGCTGATGTCTGGTGGGGACATGAAGAGCTTTTTGAGGCACAGCAGACCACACCCAGGACA ACTGGCACCTCTGACCATGCAGGACCTATTGCAGCTGGCCCAGGATATAGCCCAGGGCTGCCACTACCTG GAGGAAAATCACTTCATTCACAGAGACATTGCTGCCCGTAACTGTCTGCTTAGCTGCAGTGGAGCCAGCC GAGTGGCCAAGATTGGAGATTTTGGAATGGCAAGAGATATCTACCAGGCCAGTTATTATCGCAAGGGTGG CCGGACCTTGCTCCCAGTCAAGTGGATGCCGCCAGAAGCTCTCCTGGAGGGCCTTTTCACATCCAAGACA GACTCCTGGTCTTTTGGGGTCCTGCTCTGGGAGATCTTCTCACTGGGGTATATGCCCTACCCTGGACATA CCAACCAGGAGGTTCTAGACTTCATTGCCACAGGGAACAGGATGGACCCTCCTAGGAACTGTCCTGGGCC AGTGTACCGAATCATGACCCAGTGTTGGCAGCATCAGCCGGAGCTCCGCCCTGACTTTGGCAGCATCTTG GAACGGATTCAGTACTGCACTCAGGACCCTGATGTGCTGAACTCACCCCTGCCCGTGGAACCTGGGCCCA TTCTAGAGGAGGAAGAGGCCTCCAGGCTGGGAAACAGGTCACTGGAGGGTCTTAGATCCCCAAAGCCCCT AGAGCTGAGTTCTCAGAACTTGAAGAGCTGGGGAGGAGGCCTTCTTGGCTCTTGGCTGCCCTCTGGCCTC AAGACCCTCAAACCCAGGTGCCTCCAACCTCAGAACATTTGGAACCCCACCTATGGCTCCTGGACCCCAA GGGGCCCCCAGGGTGAAGATACAGGCATTGAACACTGCAATGGCTCCTCCTCAAGTTCCATTCCAGGCAT CCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222629 representing NM_203345
Red=Cloning site Green=Tags(s) MGCSHRLLLWLGAAGTILCSNSEFQTPFLTPSLLPVLVLNSQEQKVTPTPSKLEPASLPNPLGTRGPWVF NTCGASGRSGPTQTQCDGAYTGSSVMVTVGAAGPLKGVQLWRVPDTGQYLISAYGAAGGKGAQNHLSRAH GIFLSAVFFLRRGEPVYILVGQQGQDACPGGSPESQLVCLGESGEHATTYGTERIPGWRRWAGGGGGGGG ATSIFRLRAGEPEPLLVAAGGGGRSYRRRPDRGRTQAVPERLETRAAAPGSGGRGGAAGGGSGWTSRAHS PQAGRSPREGAEGGEGCAEAWAALRWAAAGGFGGGGGACAAGGGGGGYRGGDTSESDLLWADGEDGTSFV HPSGELYLQPLAVTEGHGEVEIRKHPNCSHCPFKDCQWQAELWTAECTCPEGTELAVDNVTCMDLPTTAS PLILMGAVVAALALSLLMMCAVLILVNQKCQGLWGTRLPGPELELSKLRSSAIRTAPNPYYCQVGLSPAQ PWPLPPGLTEVSPANVTLLRALGHGAFGEVYEGLVTGLPGDSSPLPVAIKTLPELCSHQDELDFLMEALI ISKFSHQNIVRCVGLSFRSAPRLILLELMSGGDMKSFLRHSRPHPGQLAPLTMQDLLQLAQDIAQGCHYL EENHFIHRDIAARNCLLSCSGASRVAKIGDFGMARDIYQASYYRKGGRTLLPVKWMPPEALLEGLFTSKT DSWSFGVLLWEIFSLGYMPYPGHTNQEVLDFIATGNRMDPPRNCPGPVYRIMTQCWQHQPELRPDFGSIL ERIQYCTQDPDVLNSPLPVEPGPILEEEEASRLGNRSLEGLRSPKPLELSSQNLKSWGGGLLGSWLPSGL KTLKPRCLQPQNIWNPTYGSWTPRGPQGEDTGIEHCNGSSSSSIPGIQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_203345 |
ORF Size | 2664 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_203345.2, NP_976220.2 |
RefSeq Size | 3115 bp |
RefSeq ORF | 2667 bp |
Locus ID | 17005 |
UniProt ID | P08923 |
Gene Summary | The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222414 | Ltk (untagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4, (10ug) |
CNY 11,500.00 |
|
MR222629 | Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4 |
CNY 6,504.00 |
|
MR222629L3 | Lenti ORF clone of Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4 |
CNY 9,690.00 |
|
MR222629L4 | Lenti ORF clone of Ltk (mGFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4 |
CNY 9,690.00 |